氧化型低密度脂蛋白促进单核细胞-内皮细胞体外粘附反应

体外培养猪胸主动脉内皮细胞，观察氧化型低密度脂蛋白对内皮细胞－单核细胞体外粘附反应的影响同时观察氧化型低密度脂蛋白对内皮细胞表面粘附分子颗粒膜蛋白－１４０及内皮细胞分泌前列环素的影响。     

氧化型低密度脂蛋白和普伐他汀对人脐静脉内皮细胞细胞间粘附分子-1表达的影响

观察氧化型低密度脂蛋白对人脐静脉内皮细胞细胞间粘附分子－１表达的诱导及普伐他汀对它的抑制影响,以期氧化型低密度脂蛋白致动脉粥样硬化的机制及普伐他汀可能的非调脂抗动脉粥样硬化作用。体外培养人脐静脉内皮细胞,分别加氧化型低密度脂蛋白５０ｍｇ／Ｌ、１００ｍｇ／Ｌ、２００ｍｇ／Ｌ及氧化型低密度旨蛋白（１００ｍｇ／Ｌ）＋普伐他汀（１０＾－４－－６ｍｏｌ／Ｌ）,孵育１２ｈ、２４ｈ和３６ｈ,采用细胞酶联免疫吸附     

内皮细胞形态和排列与血液动力学关系的探讨

丙丁酚阻抑氧化低密度脂蛋白介导的内皮细胞－单核细胞粘附作用李立新，陈剑雄，余麟，廖端芳，黄红林（衡阳医学院心肺药理研究室，衡阳４２１００１）用氧化低密度脂蛋白处理培养的猪胸主动脉内皮细胞，观察内皮细胞表面颗粒膜蛋白－１４０、内皮细胞－单核细胞粘附率、...     

L-精氨酸抑制内皮细胞粘附分子-1表达与氧化型低密度脂蛋白促单核细胞粘附作用下降有关

用氧化型低密度脂蛋白处理培养的猪主动脉内皮细胞，以Ｆｉｃｏｌｌ－Ｈａｐａｑｕｅ分离液密度梯度离心分离人外周血单核细胞，观察Ｌ－精氨酸对内皮细胞－单核细胞粘附率的影响；采用反转录－多聚酶链反应技术检测氧化型低密度脂蛋白和Ｌ－精氨酸对内皮细胞血管细胞粘附分子－１表达的影响。结果显示：Ｌ－精氨酸具有剂量和时间依赖性地抑制氧化型低密度脂蛋白的促内皮细胞－单核细胞粘附作用。氧化型低密度脂蛋白处理内皮细胞能明显增加内皮血管细胞粘附分子。－１的表达，而Ｌ－精氨酸阻抑氧化型低密度脂蛋白的此种作用。结果提示Ｌ－精氨酸可能通过抑制血管细胞粘附分子－１表达阻抑氧化型低密度脂蛋白的促内皮细胞—单核细胞粘附作用。     

L-精氨酸抑制内皮细胞粘附分子-1表达与氧化型低密度脂蛋白促单核细胞粘附作用下降有关

用经型低密度脂蛋白处理培养的猪主动脉内皮细胞，以Ｆｉｃｏｌｌ－Ｈａｐａｑｕｅ分离浓密度梯度离心分离人外周血单核细胞，观察Ｌ－精氨酸对内皮细胞－单核细胞粘附率的影响，采用反转录－多聚酶链反应技术检测氧化型低密度脂蛋白和Ｌ－精氨酸对内皮细胞血管细胞粘附分子－１表达的影响。结果显示：Ｌ－精氨酸具有剂量和时间依赖性地抑制氧化型低密度脂白促内皮细胞－单核细胞粘附作用，结果提示Ｌ－精氨酸可能通过抑制血管细胞粘     

冠心病患者血浆血小板颗粒膜蛋白水平及其临床意义

为了进一步研究血小板活化与冠心病的关系，应用ＥＬＩＳＡ法检测６０例冠心病（ＣＡＤ）患者血浆血小板颗粒膜蛋白（ＧＭＰ－１４０）水平，正常对照按年龄分为Ⅰ组、Ⅱ组。结果：急性心肌梗塞（ＡＭＩ组），不稳定心绞痛（ＵＡ组）、稳定型心绞痛（ＳＡ组）三组患者血浆ＧＭＰ－１４０水平分别为１５．４±１３．３、１３．２±６．０、８．０±６．０ｎｇ／ｍｌ，均明显高于Ⅰ组或Ⅱ组。ＵＡ组血浆ＧＭＰ－１４０同于ＳＡ组，与Ａ     

氧化型低密度脂蛋白介导单核细胞与血管内皮细胞粘附的单细胞定量研究

利用细胞微管吸吮技术对单核细胞与血管内皮细胞间的粘附力行单细胞定量测量，以两种细胞间的临界分离应力Ｓｃ来表示细胞的粘附性。观察氧化型低密度脂蛋白对单核细胞与培养人脐静脉内皮细胞粘附的影响。结果显示：氧化型低密度脂蛋白可使内皮细胞对正常单核细胞的粘附性明显增大，其发生较缓慢，４ｈ达峰值并维持至２４ｈ以后。经氧化型低密度脂蛋白处理的内皮细胞对中性粒细胞的粘附性无明显提高。单核细胞和中性粒细胞经氧化型低密度脂蛋白处理后，对内皮细胞的粘附性均有迅速提高，１ｈ达峰值，２４ｈ内逐渐减弱。实验结果提示氧化型低密度脂蛋白可选择性地提高单核细胞与内皮细胞相互粘附性，这种选择性的促粘附作用进一步证明它在动脉粥样硬化发生、发展中起重要作用。     

冠状动脉粥样硬化斑块组成成分与斑块破裂的关系

为观察氧化型低密度脂蛋白对内皮细胞一氧化氮生成的影响,采用体外细胞培养方法将含不同浓度氧化型低密度脂蛋白的ＤＭＥＭ 培养液（ 氧化型低密度脂蛋白终浓度分别为０ ｍｇＬ、５０ ｍｇＬ 、１００ ｍｇＬ 、２００ ｍｇＬ）,与内皮细胞共同孵育（３７ ℃、５％ ＣＯ２）２４ ｈ 后,分别测上清液中乳酸脱氢酶活性和一氧化氮含量及细胞中一氧化氮合酶活性,并用细胞化学染色法记数各组细胞一氧化氮合酶阳性染色细胞数。结果发现,随着培养液中氧化型低密度脂蛋白浓度升高,上清液中乳酸脱氢酶活性增加和一氧化氮含量下降,细胞中一氧化氮合酶活性和一氧化氮合酶阳性染色细胞数下降,可见,氧化型低密度脂蛋白可损伤血管内皮细胞,降低细胞中一氧化氮合酶活性,抑制一氧化氮产生,这可能是氧化型低密度脂蛋白致动脉粥样硬化的原因之一。     

氧化型低密度脂蛋白对培养的内皮细胞一氧化氮合成和释放的影响

为观察氧化型你 蛋白对内此一氧化氮生成的影响，采用体外细胞培养方法钭含有不同深度氧化型低宽度脂蛋白的ＤＭＥＭ培养液（氧化型低密度脂蛋白终浓度分别为０ｍｇ／Ｌ、５０ｍｇ／Ｌ、１００ｍｇ／Ｌ、２００ｍｇ／Ｌ），与内皮细胞共同孵育（３７℃、５％ＣＯ２）２４后，分别测上清液中乳酸脱氢酶生和一氧化氮含量及细胞中一氧化氮合酶活性，并用细胞化学染色法记数各组细胞一氧化氮合酶阳性染色细胞数。结果发现，随着陪送２液     

植物杀螺剂麻风树籽杀螺筛选的报告

对麻风树籽（ＪＣＳ）不同制剂杀灭钉螺筛选实验观察，发现２ 号粉剂和６ 号水剂具有良好的杀钉螺效果。２ 号浓度１０ｍｇ／Ｌ，６ 号浓度６－２５ｍｇ／Ｌ，浸杀钉螺７２ｈ 死亡率均为１００－０ ％ 。现场喷洒剂量６ｇ／ｍ２ ，５ｄ 钉螺死亡率均为１００－０％ 。２ 号粉剂急性鱼类毒性试验结果ＬＣ５０ 为６９６－３１ｍｇ／Ｌ。该两种制剂进一步提纯和定型后，具有大批量生产和在疫区推广使用的价值。     

Pathogenesis of carcinoma of the papilla of Vater

Most adenomas and carcinomas of the small intestine and extrahepatic bile ducts arise in the region of the papilla of Vater. In familial adenomatous polyposis (FAP) it is the main location for carcinomas after proctocolectomy. In many cases symptoms due to stenosis lead to diagnosis at an early tumor stage. In about 80%, curative intended resection is possible. Operability is the most relevant prognostic factor. Most ampullary carcinomas resp. carcinomas of the papilla of Vater develop from adenomatous or flat dysplastic precursor lesions. They can be sited in the ampulloduodenal part of the papilla of Vater, which is lined by intestinal mucosa. They also can develop in deeper parts of the ampulla, which are lined by pancreaticobiliary duct mucosa. Intestinal-type adenocarcinoma and pancreaticobiliary-type adenocarcinoma represent the main histological types of ampullary carcinoma. Furthermore, there exist unusual types and undifferentiated carcinomas. Many carcinomas of intestinal type express the immunohistochemical marker profile of intestinal mucosa (keratin 7?, keratin 20+, MUC2+). Carcinomas of pancreaticobiliary type usually show the immunohistochemical profile of pancreaticobiliary duct mucosa (keratin 7+, keratin 20?, MUC2?). Even poorly differentiated carcinomas, as well as unusual histological types, may conserve the marker profile of the mucosa they developed from. These findings underline the concept of histogenetically different carcinomas of the papilla of Vater which develop either from intestinal- or from pancreaticobiliary-type mucosa of the papilla of Vater. Molecular alterations in ampullary carcinomas are similar to those of colorectal as well as pancreatic carcinomas, although they appear at different frequencies. In future studies, molecular alterations in ampullary carcinomas should be correlated closely with the different histologic tumor types. Consequently, the histologic classification should reflect the histogenesis of ampullary tumors from the two different types of papillary mucosa.     

Demonstration of the mechanism of action of biguanides

Summary Palmitic acid oxidation in rat diaphragm homogenate is depressed by biguanide concentrations that are still incapable of inhibiting oxidative phosphorylation. Glucose oxidation is not directly effected by the same biguanide concentrations: however, the inhibitory effect of palmitic acid on glucose oxidation is partly removed by biguanides. Inhibition of fatty acid oxidation, which accounts for most of the metabolic effects caused by these drugs, can be regarded as the fundamental mechanism of action of biguanides. There is some evidence suggesting that these drugs might interact with carnitine, thus preventing long-chain fatty acids from being transported across the mitochondrial membrane to the site of oxidation. Traduzione a cura degli AA.     

胰岛素瘤的解剖分布特点分析

目的胰岛素瘤是最常见的胰腺神经内分泌肿瘤，因其临床表现多样，导致诊断困难。影像学诊断尤其是超声内镜(EUS)在胰岛素瘤的诊断中起着重要作用，拥有较高的敏感性和特异性。本研究拟通过明确胰岛素瘤的解剖分布特点，以期有助于提高影像学的诊断准确率和降低漏诊率，尤其是在教育和培训实践中对于EUS的学习者更具有指导价值。 方法回顾性分析解放军总医院第一医学中心病案资料数据库1993年1月至2019年11月经外科手术、病理确诊为胰岛素瘤的患者的临床资料，检索方法采取搜索术后病理诊断为"胰岛素瘤"的病例，通过查阅病例的方法，提取出胰岛素瘤的大小和解剖分布等数据，进一步分析其特点。 结果共检索到确诊为胰岛素瘤的患者116例，其中，男45例、女71例，年龄13～76岁，平均年龄(44.4±14.85)岁。胰岛素瘤单发110例(94.8%)、多发6例(5.2%)。位置分布：头颈部46例(39.7%)，单发45例、多发1例；体尾部68例(58.6%)，单发65例、多发3例；全胰腺多发2例(1.7%)。病变大小特点：最大径0.4～3.4 cm，平均大小(1.53±0.58)cm。≤1 cm 29例、>1 cm而≤1.5 cm41例、>1.5 cm而≤2.0 cm28例，≤3 cm 15例，>3 cm 3例。年龄与肿瘤的大小相关，≤44岁患者肿瘤平均大小为(1.36±0.51)cm、>44岁患者肿瘤平均大小为(1.70±0.60)cm，P<0.05。头颈部的肿瘤大于体尾部的肿瘤，头颈部肿瘤平均大小(1.66±0.63)cm，体尾部(1.42±0.52)cm，P<0.05。 结论胰岛素瘤在胰腺体尾部较头颈部更好发；绝大多数单发，但可以全胰腺多发；多数小于1.5 cm，肿瘤的大小与患者年龄和肿瘤的解剖分布相关。     

氯硝柳胺悬浮剂的毒性评价

目的评价氯硝柳胺悬浮剂的毒性，为现场大规模应用灭螺提供依据。方法按照中华人民共和国国家标准GB 15670-1995《农药登记毒理学试验方法》和鱼类毒性试验方法进行。结果经口、经皮肤的LDso雌、雄性大鼠均>5 000 mg/kg，经呼吸道的LCso雌、雄性大鼠均>5 000mg/m3，该药经口、经皮肤、经呼吸道毒性均属微毒类药物；兔眼用药后，观察期内无不良反应，对眼无刺激性；皮肤用药后对皮肤无刺激性。与氯硝柳胺原药、氯硝柳胺乙醇胺盐原药和氯硝柳胺乙醇胺盐可湿性粉剂相比，氯硝柳胺悬浮剂对鱼急性毒性最低。结论氯硝柳胺悬浮剂属微毒类药物，对鱼的毒性低于其乙醇胺盐可湿性粉剂，适合于现场应用。     

Lack of correlation of degree of villous atrophy with severity of clinical presentation of coeliac disease

BACKGROUND AND AIM: Both the clinical presentation and the degree of mucosal damage in coeliac disease vary greatly. In view of conflicting information as to whether the mode of presentation correlates with the degree of villous atrophy, we reviewed a large cohort of patients with coeliac disease. PATIENTS AND METHODS: We correlated mode of presentation (classical, diarrhoea predominant or atypical/silent) with histology of duodenal biopsies and examined their trends over time. RESULTS: The cohort consisted of 499 adults, mean age 44.1 years, 68% females. The majority had silent coeliac disease (56%) and total villous atrophy (65%). There was no correlation of mode of presentation with the degree of villous atrophy (p=0.25). Sixty-eight percent of females and 58% of males had a severe villous atrophy (p=0.052). There was a significant trend over time for a greater proportion of patients presenting as atypical/silent coeliac disease and having partial villous atrophy, though the majority still had total villous atrophy. CONCLUSIONS: Among our patients the degree of villous atrophy in duodenal biopsies did not correlate with the mode of presentation, indicating that factors other than the degree of villous atrophy must account for diarrhoea in coeliac disease.     

血吸虫童虫生长发育及其机制的研究进展

血吸虫童虫是宿主免疫系统攻击的重要靶标,包括皮肤型、肺型和肝门型童虫。宿主分子对童虫生长发育具有重要作用。童虫生长发育机制包括免疫调节、信号转导、性别发育及凋亡等。肌动蛋白、组织蛋白酶、烯醇化酶和葡萄糖基转移酶等分子为血吸虫童虫生长发育的重要分子。本文对血吸虫童虫生长发育及其机制的研究进展做一综述。     

124例耐多药结核分枝杆菌基因突变特征分析

目的对临床分离的耐多药结核分枝杆菌相关基因的突变特征进行分析。方法对124例耐多药结核分枝杆菌以及50株敏感株的耐药相关基因(包括异烟肼inh A、kat G、oxyR-ahp C间隔区以及利福平rpo B)进行序列测定,分析其基因突变情况。结果异烟肼耐药inh A基因突变率为14.5%;kat G基因突变率为70.2%(87/124),主要位于315位;oxyR-ahp C间隔区突变率为15.3%;inh A、kat G两种基因同时突变率75.0%,三种基因同时突变率为89.5%。利福平rpo B基因突变的检出率高达95.2%,突变主要发生在531、526、516位点。结论我省耐多药菌异烟肼耐药相关基因最常见突变为kat G 315、inh A C-T(-15)、axyR-ahp C间隔区(-10)C-T,利福平为rpo B531、526、516。结合MDR-TB耐药相关基因的特征分析,可以建立一种快速、准确、特异的适合于我省的检测结核菌耐多药性的新方法。     

Assessment of quality of life of parents of children with juvenile chronic arthritis

The aim of the study was to assess the quality of life (QOL) and the psychological status of parents of children with juvenile chronic arthritis (JCA). The QOL, anxiety and depression of the parents of 28 children with JCA were evaluated and compared to those of the parents of 28 healthy children. Mothers of JCA children and mothers of healthy children reported similar QOL. The reported anxiety and depression levels were similar for mothers and fathers in both groups. The parents of children with pauciarticular-type JCA reported lower QOL and higher levels of anxiety and depression than the parents of children with other types, namely polyarticular and systemic JCA. These findings may be explained by the fact that the pauciarticular patients had shorter disease duration and were less frequently seen in the outpatient clinic. The QOL of mothers of children with JCA was found to be slightly impaired in the group of children with pauciarticular JCA. Future larger studies are needed to confirm these results, as the number of subjects in the three groups was rather low. Received: 26 September 2001 / Accepted: 8 February 2002     

CagA-Hp抗体IgG与胃炎的组织学分析

研究幽门螺杆菌(Hp)感染与胃炎的关系。方法对204例慢性胃炎患者胃粘膜进行观察分析,并测定其中137例Hp阳性患者血清CagA-Hp抗体IgG水平,与组织学对照。结果慢性萎缩性胃炎伴肠上皮化生患者血清CagA抗体IgG明显高于对照组(P＜0.01);其他类型胃炎患者血清CagA抗体IgG水平无明显增高(P＞0.05)。结论CagA-Hp可能是导致慢性萎缩性胃炎伴肠上皮化生的因素之一,对这类患者应密切随访观察。     

慢阻肺急性加重期患者预后的影响因素分析

目的探讨慢性阻塞性肺病急性加重期(AECOPD)患者预后的相关危险因素。方法回顾性调查、收集58例AECOPD患者可能影响其预后的相关因素,并对其分别进行单因素分析。并进行Logistic多元逐步回归进行多因素分析,筛选影响AECOPD患者预后的独立危险因素。结果单因素分析后将结果 P0.1的因素纳入多因素Logistic回归,分析发现是否合并呼吸衰竭、气促程度、白细胞计数、APACHEⅡ、应用抗氧化剂、慢阻肺治疗依从性为影响AECOPD患者预后不佳的独立因素(P0.05)。结论根据AECOPD患者预后的独立危险因素,及早判断,选择合适的后续治疗方案,对提高其生存率及生存质量具有重要意义。