导数-比导数波谱法同时测定环境水样中硝酸根和亚硝酸根的含量

为简化生活饮用水源中硝酸根和亚硝酸根的检测。去除水中的CO3 2 及浊度的干扰 ,以“减法技术”求导 ,用导数 比导数波谱法测定环境水样中硝酸根和亚硝酸根含量。结果表明本法很好地消除了导数光谱值较小的物质及仪器噪声带来的干扰。回收率为 96.7%～ 10 2 .5 %。     

导数—比导数波谱法同时测定环境水样中硝酸根和亚硝酸根？…

为简化生活饮用水源中硝酸根和亚硝酸根的检测。去除水中的ＣＯ３２－及浊度的干扰，以“减法技术”求导，用导数－比导数波谱法测定环境水样中硝酸根和亚硝酸根含量。结果表明本法很好地清除了导数光谱值较小的物质及仪器噪声带来的干扰。回收率为９６．７％－１０２．５％。     

反相高效液相色谱法测定卤肉制品中硝酸盐及亚硝酸盐

目的采用反相高效液相色谱-二极管阵列检测器法测定卤肉制品中硝酸盐及亚硝酸盐含量。方法选择四丁基溴化铵（TBABr）作为离子对试剂，与硝酸根和亚硝酸根阴离子形成中性缔合物，在甲醇-1．25mmol／L混合磷酸盐流动相中，被非极性键合相柱（ODS）分离并定量检山。结果在此检测条什下硝酸盐和亚硝酸盐有很好的线性，相关系数均人于0．9999，两者的最小检出限分别为0．01mg／L和0．005mg／L，相对标准差均小于5％，回收率在86．3％-99．1％之间。结论该法适用于卤肉制品中硝酸盐及亚硝酸盐含量的同时测定。     

唾液中亚硝酸根含量与鼻咽癌的相关性研究

目的测定鼻咽癌患者唾液中亚硝酸根含量,探讨其与鼻咽癌的相关性。方法选择不同地域、不同人群中鼻咽癌患者29例为实验组,健康人30例为对照组,测定两组人员唾液中亚硝酸根的含量。结果对照组唾液中亚硝酸根含量为(43.34±3.17)μmol/L,男女间无显著性差异(P>0.05)。实验组唾液中亚硝酸根含量为(68.54±3.44)μmol/L,显著高于对照组(P<0.01)。结论鼻咽癌患者唾液中亚硝酸根含量高于健康人,提示唾液中较高浓度的亚硝酸根含量可能是鼻咽癌病因因素之一。     

亚硝酸根与中性红显色反应的分光光度研究及其分析应用

本文研究了亚硝酸根与中性红显色反应的条件。在0.09～0.4mol/L HCl介质中,亚硝酸根与中性红生成紫蓝色化合物,其最大吸收峰位于535nm处,反应灵敏(∑_(645)nm=7.40×10~3和9.81×10~3L·mol~(-1)·cm~(-1),选择性好。亚硝酸根含量在0～5μg/25ml及5～25μg/25ml范围内遵守比尔定律。本法曾用于水、土壤及蔬菜中微量亚硝酸根的测定获得满意的结果。     

水和蔬菜中硝酸根的比色测定

1、本文报告了用二磺酸酚试剂测定硝酸根的合适比色条件以及该试剂的制备方法并拟定了水、土壤和蔬菜中硝酸根的测定步骤。 2、文中讨论了氯根、亚硝酸根和磷酸根对硝酸根测定的影响以及氯根的除去方法。 3、文中报告了本地区11种水样、2种土壤和15种蔬菜中硝酸根的含量。 4、本法优点是试剂易制备、操作简便快速,准确性能符合一般分析要求。     

二阶导数荧光法同时测定人血清中血红蛋白和白蛋白含量

目的:建立同时测定人血清中血红蛋白和白蛋白含量的方法.方法:在pH 7.4的条件下,测定人血清中血红蛋白和自蛋白的二阶导数荧光光谱,采用峰零交叉测量法,同时求血红蛋白和白蛋白的含量.结果:血红蛋白测定的线性范围为2.0×10<'-7>～12.0×10～mol/L,r=O.997,回收率为96.6%～100.1%;白蛋白...     

用甲基蓝分光光度法测定亚硝酸根

目的:研究亚硝酸根与甲基蓝的褪色反应,建立一个简单、灵敏、选择性高的亚硝酸根的测定方法。方法:采用紫外分光光度法测定不同条件下的吸光度。结果:实验表明,当甲基蓝与NO2-反应后发生颜色变化,在590 nm波长处发生褪色反应,此长波处,吸光度的变化与溶液中NO2-的浓度成正比,其线性范围在0~5 m g/L,摩尔吸光系数为ε=1.4×104L.m ol-1.cm-1,相关系数为0.995 5。结论:甲基蓝分光光度法具有良好的稳定性和选择性,用于环境水样中微量NO2-的测定,结果满意;建立了测定亚硝酸根的新方法。     

紫外分光光度法测定微量亚硝酸根

目的: 研究硫堇与亚硝酸根的反应,建立一个简单、灵敏、选择性高的微量NO-2的测定方法.方法: 用紫外分光光度法测定微量NO-2.结果: 在盐酸介质中,硫堇在近紫外区有一强吸收峰位于284 nm,与NO-2反应后284 nm处吸收峰下降,吸光度的变化与溶液中NO-2的浓度成正比,其线性范围在0～0.8 mg/L NO-2,摩尔吸光系数为ε284=2.9×104 L/(mol*cm).研究了反应的适宜条件,共存物质的影响,表明该测定方法具有良好的稳定性和选择性.结论: 用紫外分光光度法测定环境水中微量NO-2,结果满意.     

催化光度法测定痕量亚硝酸根

基于亚硝酸根对氯酸钾氧化溴甲酚绿而使其褪色所起的催化作用，建立了高灵敏度催化光度法测定痕量亚硝酸根的新方法，测定范围为０．０８～０．６４μｇ／ｍｌ，用于硝酸钾和唾液中亚硝酸根的测定，结果满意。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.