常见性传播疾病患者人巨细胞病毒感染状况的调查分析

目的调查性病患者巨细胞病毒感染状况,探讨性病与巨细胞病毒(HCMV)感染的关系。方法采用酶联免疫吸附法(ELISA),检测2组调查对象(性病患者、健康对照组)血清中HCMV-IgM、IgG特异抗体,测定其活动性感染、既往性感染和总感染率。结果性病组的总感染率分别为94.73%,与对照组58.50%相比较P<0.01。各类性病总感染率分别为97.14%、94.67%、94.76%、94.64%、92.86%,分别与对照组相比较P<0.01。结论性病与HCMV感染之间存在密切联系。HCMV通过性传播途径感染,必须加强对未生育的性病患者或经常不良性行为者进行HCMV感染的检测,达到优生优育的目的。     

解脲支原体和沙眼衣原体感染是导致妇女不孕的重要因素

目的研究不孕症女性解脲支原体(UU)和沙眼衣原体(CT)的感染状况及其与不孕症的关系。方法对318例患不孕症的女性(不孕组)和251例正常生育的女性(对照组)进行宫颈分泌物的UU和CT检测,比较两组女性UU与CT感染率差异,并分析原发性不孕症与继发性不孕症女性感染率的差异。结果不孕组UU、CT感染检出率分别为30.6%、8.7%,明显高于对照组的9.0%和0.9%(P0.05);原发性不孕女性宫颈分泌物UU、CT感染检出率分别为11.8%、3.8%,而继发性不孕女性宫颈分泌物UU、CT感染检出率分剐为40.8%、11.7%,UU感染检出率明显高于原发性不孕女性(P0.05),CT感染检出率明显高于原发不孕女性(P0.05)。结论 UU和CT与女性不孕密切相关,其感染是导致女性不孕的重要因素。     

继发不孕症和早期妊娠妇女宫颈沙眼衣原体感染的研究

目的 :了解继发不孕症和早期妊娠妇女宫颈分泌物沙眼衣原体 (CT)的感染率。方法 :收集早期妊娠、继发不孕症及对照组妇女宫颈分泌物 ,用CT PCR试剂盒进行CT DNA检测。结果 :早期妊娠组和继发不孕组CT感染率(13.33%和 14.2 8% )明显高于对照组 (3.33% ) (均P <0 .0 5 ) ,且前二者间无统计学差异 (P >0 .0 5 )。结论 :早期妊娠和继发不孕妇女均有较高的CT感染率 ,临床医生对需再生育的早孕妇女应常规作CT检测和围术期治疗。     

抗精子抗体和抗子宫内膜抗体的检测

目的　探讨抗精子抗体 (AsAb)和抗子宫内膜抗体 (EMAb)在原发性不育不孕症患者中的检出率。方法　采用ELISA检测 3 5例不育症 (不育组 )、3 0例不孕症 (不孕组 )患者和 2 4例正常对照者 (对照组 )血清中的AsAb和EMAb。结果　在 65份血清中 ,AsAb总检出率为 5 8.46% ,三组AsAb总检出率差异有高度显著性 (χ2 =18.3 3 5 ,P <0 .0 1)。不孕组EMAb总检出率为 40 .0 0 % ,以IgM -EMAb检出率为最高 ,占 2 6.67%。 结论　AsAb的存在是导致免疫性不育不孕的主要原因     

不孕症患者UU、CT感染率分析

目的探讨不孕症患者宫颈分泌物中解脲支原体（UU）和沙眼衣原体（CT）感染率及其与不孕症关系。方法采用荧光定量聚合酶链反应（PCR）技术检测590例不孕患者（不孕组）UU、CT感染率,与305例健康生育者（对照组）UU、CT感染率比较,并作相关性分析。结果不孕组宫颈分泌物UU阳性率为：45．76％,CT阳性率为15．08％,混合感染阳性率为4．58％。对照组UU阳性率为26．56％,CT阳性率为3．9％,混合感染阳性率为0．3％。不孕组UU、CT感染率比对照组明显增多,两者差异有统计学意义（P＜0．01）。结论UU、CT感染与不孕症密切相关,UU、CT应成为不孕症检查的常规项目。     

解脲支原体感染对男女不育的影响

目的探讨解脲支原体（Uu）感染与男女不育不孕发生的相关性。方法以457例女性不孕症患者及其配偶259名作为观察组,353名有正常生育史的育龄妇女及其配偶282名作为对照组;取生殖道分泌物采用PCR方法检测两组标本中Uu的感染情况。结果观察组分泌物中Uu阳性率分别为50.5%和48.3%,对照组分别为15.6%和13.5%。两组比较,差异均有统计学意义（P〈0.01）。结论Uu感染与男性不育和女性不孕症发生有密切关系。     

支原体致不育不孕的研究

目的　研究解脲支原体和人型支原体致夫妇不育不孕的作用。方法　收集 1 995— 2 0 0 2年我院妇科和泌尿科不育不孕患者的精液和宫颈拭子进行“金标准”培养 ,并结合临床进行综合分析。结果　男性不育患者阳性率为 67.60 % ,不孕患者阳性率为 60 .92 % ,夫妻共患两型支原体阳性率为 57.39% ,对照组男性阳性率为 1 9.0 1 % ,女性阳性率为 1 .76 % ,与对照组相比P <0 .0 1 ,说明支原体感染与不育不孕有关。结论　支原体属性传播疾病 ,由此引发的不育不孕和炎症及精子异常与支原体感染有密切关系。为性病防治制定措施提供了可靠依据。     

不孕症妇女的沙眼衣原体与解脲支原体检测

目的：了解不孕症妇女宫颈分泌物沙眼衣原体(CT)、解脲支原体(UU)的感染情况。方法：收集不孕症及正常对照组妇女宫颈分泌物，用PCR试剂盒检测CT—DNA、UU—DNA。结果：不孕组和对照组，CT阳性率分别为82．56％和17．39％；UU阳性率分别为75．58％和13．04％；CT、UU混合感染率分别为67．44％和8．70％。两组间差异均有显著性(P＜0．01)。结论：CT或／和UU感染与不孕症密切相关，临床医生应做常规检查。     

不孕症夫妇解脲支原体沙眼衣原体感染率分析

目的探讨不孕症夫妇解脲支原体（uu）和沙眼衣原体（CT）感染率及其与不孕症的关系。方法分别采用培养法和免疫学方法检测148对不孕症夫妇（不孕组）uu、CT感染率,与53对健康生育夫妇（对照组）uu、CT感染率比较,并作相关性分析。结果不孕组中妻子uu阳性率为39．19％,CT阳性率为20．95％,UU、CT混合感染（UU＋CT）阳性率为10．81％;对照组中妻子uu阳性率为13．21％,CT阳性率为7．55％,UU＋CT阳性率为1．89％,2组间差异有统计学意义（P〈0．05）。不孕组中丈夫uu阳性率为35．14％,CT阳性率为18．24％,UU＋CT阳性率为8．11％;对照组中丈夫UU阳性率为9．43％,CT阳性率为5．66％,UU＋CT阳性率为0％,2组间差异有统计学意义（P〈0．05）。不孕夫妇间uu和CT感染与不孕症均存在相关性（P〈0．001）。结论uu、CT感染与不孕症密切相关,uu、CT检测应成为不孕症检查的常规项目。     

不孕不育患者沙眼衣原体感染状况分析

目的:研究广东地区不孕不育患者沙眼衣原体(CT)的感染状况。方法:收集937例不孕不育患者(包括原发不孕不育、继发不孕不育和自然流产病例)的生殖道分泌物标本以及879例正常生育者生殖道分泌物标本,并用胶体金免疫沉析法检测CT抗原。结果:不孕不育组CT感染率为14.94%,显著高于对照组(2.62%);不孕不育女性CT感染率为17.62%,显著高于不育男性的CT感染率(10.93%)。女性原发性不孕、继发性不孕及反复自然流产的CT阳性率分别为16.24%、19.12%、16.67%,三组CT阳性率无统计学意义;男性原发性不育组、继发性不育组的CT性率分别为10.06%、11.65%,差异也无统计学意义。结论:CT感染是引起广东地区男性不育,女性不孕及自然流产的重要病因之一。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.