MDR-1和GFAP蛋白在难治性癫痫脑组织的表达

目的：观察不同病因的难治性癫痫手术切除脑组织中多药耐药基因蛋白（MDR-1）和胶质纤维酸性蛋白（GFAP）的表达。方法：在对22例难治性癫痫临床病理资料分析的基础上，应用免疫组化和免疫组化双标技术观察脑组织中MDR-1和GFAP蛋白的表达情况。结果：反应性胶质细胞增生是难治性癫痫共同的病理学特征。MDR-1蛋白的表达主要在一些增生性星形胶质细胞和毛细血管壁周围结构，而寡突胶质细胞、小胶质细胞及正常的神经元内无MDR-1蛋白的表达；增生性星形胶质细胞内MDR-1与GFAP具有共存现象。结论：在难治性癫痫的反应必脑胶质细胞内同时具有GFAP和MDR-1蛋白的高表达和共存。

The expression of MDR-1 and GFAP at the resected brain tissues of intractable epilepsy

Objective To observe the expression of GFAP and MDR 1 at the resected brain tissues of intractable epileptic patients with varied etiologies.Methods The expression of MDR 1 and GFAP in the surgically resected brain tissues was observed by single and double immunohistochemistry in 22 cases with medically intractable epilepsy.Results The presence of reactive astrocyte seemed to be a common pathological characteristic, though the etiology and clinical performance for each subject were vary. Immunoreactivity of both MDR 1 and GFAP was mainly showed at reactive astrocyte and some areas around micro vessels. Collocalization of MDR 1 and GFAP was also present at most of reactive astrocytes and the areas surrounding micro vessels. Conclusions Reactive astrocyte is one of the most common pathological characteristics in intractable epilepsy. The collocalization of overexpressed MDR 1 and GFAP at some reactive astrocyte is also prominent in brain tissues of intractable epilepsy.