还原型谷胱甘肽对缺氧/复氧培养大鼠心肌细胞的干预性研究

目的：探讨还原型谷胱甘肽（GSH）对缺氧/复氧培养大鼠心肌细胞损伤的保护作用。方法：将原代培养的乳鼠心室肌细胞分为正常对照与GSH不同浓度处理组，缺氧、复氧前均给予0、40、80、160mg/L浓度的GSH，缺氧2 h，复氧1 h。应用黄嘌呤氧化酶法测定超氧化物歧化酶（SOD）活性，用硫代巴比妥酚显色法测定丙二醛（MDA）含量。结果：与对照组相比,单纯缺氧/复氧（0 mg/L）组SOD活性显著下降、MDA水平显著升高（P＜0.01）。GSH预处理以剂量依赖性减轻细胞活力下降及MDA的上升,而对SOD的作用则在160 mg/L组达到峰值。结论：GSH对缺氧/复氧损伤的心肌细胞具有保护作用,其机制可能与GSH的抗氧化作用有关。

Reduced glutathione protects against anoxia/reoxygenation injury of cultured myocardial cells in rats

Objective:To investigate the protective effect of reduced glutathione sodium(GSH)on anoxia/reoxygenation injury of myocardial cells in rats.Methods:Primary cultured myocardial cells of rats were randomly allocated to control group and GSH groups which were respectively given 0,40,80,160 mg/L GSH before 2-hour anoxia or 1-hour reoxygenation.Before anoxiated,at 2 hours after anoxiated,and at 1 hour after reoxygenated,the activities of superoxide dismutase(SOD)were measured by the method of xanthine oxidase,and the contents of serum malonicaldehyde(MDA)were determined by the method of thiobarbituric acid.Results:Compared with control,the activity of SOD was markedly decreased,while the content of cellular MDA was significantly increased in 0 mg/L GSH of anoxia/reoxygenation group(P<0.01).GSH in a dose-dependent manner could significantly decrease the reduced SOD activity and the ascending MDA,and GSH in 160 mg/L had the greatest protective effect on SOD.Conclusion:GSH protects the myocardial cell from anoxia/reoxygenation injury,which may be related to the ability of GSH in against oxidant injury.