川芎嗪预处理对大鼠肾脏缺血再灌注损伤的保护作用

目的:探讨川芎嗪预处理对大鼠肾脏缺血再灌注损伤的保护作用及机制。方法:30只SD大鼠被随机分为3组(n=10):假手术组、缺血再灌注损伤(ischemia reperfusion injury,IR)组、川芎嗪预处理组(ligus-trazine injection preconditioning group,LI)。建立大肾脏缺血再灌注损伤模型,测定血清肌酐(Scr)与尿素氮(BUN)含量、肾组织匀浆中SOD(黄嘌呤氧化酶法)、GSH_Px(二硫代二硝基甲苯法)的活性及MDA(硫代巴比妥酸盐法)含量。结果:与缺血再灌注损伤(IR)组比较,川芎嗪预处理(PFI)组Scr(P<0.01)、BUN(P<0.05)、MDA(P<0.05)含量显著下降。而SOD(P<0.01)、GSH_Px(P<0.05)活力显著升高。结论:SOD、GSH_Px是重要的内源性保护物质,川芎嗪可增加SOD、GSH_Px的活性、增加机体抗氧化和清除自由基的能力,从而发挥对肾缺血再灌注损伤的预防和保护作用。

Protective effects of ligustrazine on the renal ischemia reperfusion injury model in rats

Objective: To investigate the protective effects and its mechanism of ligustrazine precondition on renal ischemia reperfusion injury in rats.Methods: 30 SD rats were divided into three groups randomly(n=10):sham operation group,ischemia reperfusion injury group(IR),and ligustrazine injection preconditioning group(LI).The model of renal ischemia reperfusion injury was produced,after renal ischemia lasting for 60 mins,then reperfusion for 24 hrs.The serum Scr and BUN levels were measured,the renal SOD and GSH_Px activities,MDA contents were detected respectively.Results: The contents of Scr(P<0.01),BUN(P<0.05),MDA(P<0.05) were significantly lower,the activities of SOD(P<0.01)and GSH_Px(P<0.05) were significantly higher in ligustrazine injection preconditioning group(SFI) than that in ischemia reperfusion injury group.Conclusion: SOD and GSH_Px are important substabces of endogenous protection,and ligustrazine can enhance SOD and GSH_Px activities,and protect renal ischemia reperfusion injury.