血府逐瘀胶囊、血塞通胶囊对溶血磷脂酸诱导的血管平滑肌细胞增殖的影响

目的观察血府逐瘀胶囊及血塞通胶囊含药血清对溶血磷脂酸（LPA）诱导的血管平滑肌细胞（VSMC）增殖效应的影响。方法采用贴块法培养大鼠主动脉VSMC并分为7组：空白血清组，血府逐瘀胶囊含药血清组，血塞通胶囊含药血清组，3组分别＋LPA组，卡托普利含药血清＋LPA组。以四甲基偶氮唑蓝（MTT）比色法观察不同含药血清对细胞增殖的影响；采用流式细胞仪分析细胞周期和DNA含量。结果空白血清＋LPA组、血府逐瘀胶囊含药血清组较空白血清组0D值升高（P〈0．05），S期指数升高（P〈0．01）。血府逐瘀胶囊含药血清＋LPA组、血塞通胶囊含药血清＋LPA组较卡托普利含药血清＋LPA组0D值降低（P〉0．05，P〈0．01）；S期指数升高（P〈0．01，P〉0．05）。结论LPA、血府逐瘀胶囊对体外培养的VSMC有促增殖作用，血塞通胶囊无促增殖作用。血府逐瘀胶囊、血塞通胶囊对LPA诱导的VSMC增殖有拮抗作用，血塞通胶囊作用更强。其作用机制部分与抑制DNA合成、阻止细胞进入S期有关。

Effect of Serums Containing Xuefuzhuyu Capsules and Xuesetong Capsules on the Proliferation of Vascular Smooth Muscle Cells Induced by Lysophosphatidie Acid

Objective To investigate the effect of the serums containing Xuefuzhuyu capsules and Xuesetong capsules on the proliferation of vascular smooth muscle cells （VSMC） induced by lysophosphatidie acid （LPA）. Methods Rat VSMCs were obtainded by tissue-piece inoculation and divided into seven groups： blank serum group, Xuefuzhuyu （Xuesetong） capsules-containing serum group, LPA＋ Xuefuzhuyu （Xuesetong, captopril） containing serum group and LPA＋blank serum group. Effects of different groups on the proliferation of VSMCs was evaluated by MTT colorimetry. Analysis of cell cycle and DNA content were performed by flow cytometry. Results The optical density （OD） and the S phase fraction （SPF） of LPA＋blank serum group and Xuefuzhuyu capsules-containing serum group were more increased than the blank serum group （P〈0.05, P〈0.01）. In comparison with LPA＋captopril containing serum group, OD of LPA ＋Xuefuzhuyu capsules-containing serum group and LPA＋Xuesetong capsulescontaining serum group was decreased （P〉0.05, P〈0.01）, SPF was increased （P〈0.01, P 〉0.05）. Conclusion Xuefuzhuyu capsules and LPA promoted the proliferation of VSMCs in vitro. Xuefuzhuyu and Xuesetong capsules can inhibit the proliferation induced by LPA, and effect of Xuesetong capsules was greater. Part of the mechanism may be associated with the inhibition of DNA synthesis and preventing the transition from G0/G1 to S phase of VSMCs.