| 亲水作用色谱-蒸发光散射检测器联用法同时测定巴戟天中5种低聚糖的含量 |
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| 引用本文： | 邓少东,肖凤霞,林励,张鹏,林靖然,张盛滨.亲水作用色谱-蒸发光散射检测器联用法同时测定巴戟天中5种低聚糖的含量[J].中国中药杂志,2012,37(22):3446-3450. |
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| 作者姓名： | 邓少东 肖凤霞 林励 张鹏 林靖然 张盛滨 |
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| 作者单位： | 广州中医药大学中药学院,广东广州,510006 |
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| 基金项目： | 海南省重点科技计划项目(090603);广东省大学生创新实验项目(1057212041) |
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| 摘 要： | 目的:采用亲水作用色谱-蒸发光散射检测器联用法同时测定巴戟天中蔗糖、蔗果三糖、耐斯糖、1 F-果呋喃糖基耐斯糖、巴戟甲素5种低聚糖的含量.方法:采用Waters XBridgeTM Amide(4.6 mm×150 mm,3.5 μm)亲水性作用色谱柱；以乙腈(A)-0.2％三乙胺溶液(B)为流动相,梯度洗脱；流速0.8 mL·min；柱温40℃；以Waters 2424 ELSD检测,漂移管温度90℃,气压275.79 kPa.结果:5种低聚糖分别在2.128 ～21.28 μg(r=0.999 3),1.864～18.64 μg(r=0.999 6),1.92 ～19.2μg(r=0.999 8),1.912～19.12 μg (r=0.999 5),2.368 ～ 23.68 μg(r=0.999 4)线性关系良好,其回收率在92.81％～102.8％ (n =6).结论:该方法简单、准确,具有良好的重复性,为有效评价巴戟天药材的质量提供了分析方法.
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| 关 键 词： | 巴戟天 亲水作用色谱 蒸发光散射检测器 蔗糖 蔗果三糖 耐斯糖 1F-果呋喃糖基耐斯糖 巴戟甲素 |
| 收稿时间： | 7/1/2012 12:00:00 AM |
HILIC-ELSD determination of five oligosaccharides contained in Morinda officinalis |
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| DENG Shao-dong,XIAO Feng-xi,LIN Li,ZHANG Peng,LIN Jing-ran and ZHANG Sheng-bin.HILIC-ELSD determination of five oligosaccharides contained in Morinda officinalis[J].China Journal of Chinese Materia Medica,2012,37(22):3446-3450. |
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| Authors: | DENG Shao-dong XIAO Feng-xi LIN Li ZHANG Peng LIN Jing-ran and ZHANG Sheng-bin |
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| Institution: | School of Chinese Herbal Medicine, Guangzhou University of traditional Chinese medicine, Guangzhou 510006, China;School of Chinese Herbal Medicine, Guangzhou University of traditional Chinese medicine, Guangzhou 510006, China;School of Chinese Herbal Medicine, Guangzhou University of traditional Chinese medicine, Guangzhou 510006, China;School of Chinese Herbal Medicine, Guangzhou University of traditional Chinese medicine, Guangzhou 510006, China;School of Chinese Herbal Medicine, Guangzhou University of traditional Chinese medicine, Guangzhou 510006, China;School of Chinese Herbal Medicine, Guangzhou University of traditional Chinese medicine, Guangzhou 510006, China |
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| Abstract: | |
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| Keywords: | Morinda officinalis HILIC ELSD sucrose 1-kestose nystose 1F-fructofuranosyinystose bajijiasu |
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