| 叠氮钠对心肌细胞活力影响的研究 |
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| 引用本文： | 关付,于波,齐国先,罗佳.叠氮钠对心肌细胞活力影响的研究[J].中国医科大学学报,2006,35(4):345-347. |
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| 作者姓名： | 关付 于波 齐国先 罗佳 |
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| 作者单位： | 中国医科大学附属第一医院循环内科,辽宁,沈阳,110001 |
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| 基金项目： | 国家自然科学基金;辽宁省教育厅资助项目 |
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| 摘 要： | 目的：观察叠氮钠对原代培养大鼠心肌细胞活力的影响。方法：研究不同浓度叠氮钠在不同时间内对原代培养的大鼠心肌细胞活力的影响。结果：叠氮钠对心肌细胞活力的抑制作用随浓度增加以及作用时间延长明显增加。1mmol／L叠氮钠孵育细胞3h，细胞发生可逆的早期凋亡，大于6h，细胞发生不可逆的死亡。结论：低剂量叠氮钠可诱导原代培养的大鼠心肌细胞发生可逆的早期凋亡。
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| 关 键 词： | 叠氮钠 心肌细胞 细胞活力 |
| 文章编号： | 0258-4646(2006)04-0345-03 |
| 收稿时间： | 2005-12-07 |
| 修稿时间： | 2005年12月7日 |
Effect of sodium azide on the viability of cultured neonatal rat cardiomyocytes |
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| GUAN Fu,YU Bo,QI Guo-xian,LUO Jia.Effect of sodium azide on the viability of cultured neonatal rat cardiomyocytes[J].Journal of China Medical University,2006,35(4):345-347. |
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| Authors: | GUAN Fu YU Bo QI Guo-xian LUO Jia |
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| Institution: | Department of Cardiology, The First Affiliated Hospital, China Medical University, Shenyang 110001, China |
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| Abstract: | Objective: To investigate the influence of sodium azide(NaN_3) on the viability of cultured neonatal rat cardiomyocytes.Methods: Primary cultured cardiomyocytes from 2-day-old Wistar rats were incubated with 0 to 100 mM NaN_3 for 0 to18 hours.The cell viability was measured.Results: The inhibitory effect of NaN_3 on cardiomyocyte viability was dose-and time-dependent.Cardiomyocytes incubated with 1 mmol/L NaN_3 for 3 hours showed an early stage of reversible apoptosis,but when the incubation period>6 hours the apoptosis was irreversible.Conclusion: Low-dose NaN_3 may induce early stage of reversible apoptosis in primary cultured rat cardiomyocytes. |
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| Keywords: | sodium azide cardiomyocyte cell viability |
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