| RP-HPLC测定川芎挥发油中藁本内酯的含量 |
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| 引用本文： | 林燕芝,唐星,毕开顺.RP-HPLC测定川芎挥发油中藁本内酯的含量[J].中国中药杂志,2004,29(2):154-157. |
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| 作者姓名： | 林燕芝 唐星 毕开顺 |
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| 作者单位： | 沈阳药科大学,药学院,辽宁,沈阳,110016 |
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| 摘 要： | 目的 :测定川芎挥发油中藁本内酯的含量。方法 :采用RP HPLC法 ,内标法定量 ,内标物尼群地平 ,HypersilODS2 色谱柱 (4.6mm× 200mm ,5 μm) ,流动相为甲醇-乙腈-水 (33∶2 1∶4 6 ) ,检测波长 275nm。 结果 :藁本内酯在 2.92～ 29.2mg·L-1线性关系良好 ,藁本内酯在川芎挥发油中的含量为 34.79% ,藁本内酯平均回收率 95 .1% ,RSD为 2.3%。结论 :本方法简便 ,准确 ,重现性好 ,可用于藁本内酯的含量测定。
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| 关 键 词： | 川芎 挥发油 藁本内酯 RP-HPLC |
| 文章编号： | 1001-5302(2004)02-0154-03 |
| 收稿时间： | 2002/11/20 0:00:00 |
Determination of ligustilide in volatile Oil from rhizome of Ligusticum Chuanxiong by RP-HPLC |
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| LIN Yan-zhi;TANG Xing;BI Kai-shun.Determination of ligustilide in volatile Oil from rhizome of Ligusticum Chuanxiong by RP-HPLC[J].China Journal of Chinese Materia Medica,2004,29(2):154-157. |
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| Authors: | LIN Yan-zhi;TANG Xing;BI Kai-shun |
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| Institution: | School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. |
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| Abstract: | OBJECTIVE: To determine Ligustilide in volatile oil from Ligustrcum chuanxiong with RP-HPLC. METHOD: ODS2 column (4.6 mm x 200 mm, 5 microm) was used and nitrendipine was used as internal standard. The mobil phase consisted methanol, acetontrile and water (33:21:46). The ligustilide was at 275 nm. RESULT: The linear range was 2.92-29.2 mg x L(-1) for ligustilide. The average recovery of ligustilide was 95.1% and RSD was 2.3%. CONCLUSION: This method is simple and can be used to determine ligustilide with satisfactory accuracy and reproducibility. |
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| Keywords: | Ligusticum chuanxiong volatile oil ligustilide RP-HPLC |
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