| RP-HPLC同时测定不同来源川芎中5种有效成分的含量 |
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| 引用本文： | 姚宗玲,杜守颖,陆洋,徐冰,陈晓兰.RP-HPLC同时测定不同来源川芎中5种有效成分的含量[J].中国中药杂志,2010,35(20):2696-2699. |
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| 作者姓名： | 姚宗玲 杜守颖 陆洋 徐冰 陈晓兰 |
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| 作者单位： | 北京中医药大学中药学院,北京,100102 |
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| 基金项目： | 国家"重大新药创制"科技重大专项(2009ZX09502-008);高等学校博士学科点专项科研基金博导类课题(20090013110007) |
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| 摘 要： | 目的:建立同时测定川芎中川芎嗪、阿魏酸、丁基苯酞、藁本内酯和丁烯基苯酞5种有效成分含量的RP-HPLC方法。方法:采用Grace Smart RP C18色谱柱,以乙腈-0.1%磷酸为流动相,梯度洗脱,DAD检测器检测,针对不同组分,选择其最大吸收波长下的峰面积,采用外标法定量。结果:川芎嗪、阿魏酸、丁基苯酞、藁本内酯和丁烯基苯酞的线性范围分别为0.008 35~0.668,0.020 6~1.648,0.012 2~0.976,0.050 75~4.06,0.015 7~1.256μg,平均加样回收率分别为101.98%,99.91%,96.94%,100.85%,99.04%。结论:该方法简便、快捷、重复性好,回收率高,可用于川芎中5种有效成分的同时测定,适用于川芎药材及其制剂的质量控制。
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| 关 键 词： | RP-HPLC 川芎 川芎嗪 阿魏酸 丁基苯酞 藁本内酯 丁烯基苯酞 |
| 收稿时间： | 2010/3/11 0:00:00 |
Simultaneous determination of five effective components in Rhizoma Chuanxiong by RP-HPLC |
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| YAO Zongling,DU Shouying,LU Yang,XU Bing and CHEN Xiaolan.Simultaneous determination of five effective components in Rhizoma Chuanxiong by RP-HPLC[J].China Journal of Chinese Materia Medica,2010,35(20):2696-2699. |
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| Authors: | YAO Zongling DU Shouying LU Yang XU Bing and CHEN Xiaolan |
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| Institution: | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing100102, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing100102, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing100102, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing100102, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing100102, China |
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| Abstract: | |
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| Keywords: | RP-HPLC method Rhizoma Chuanxiong ligustrazine ferulic acid butylphthalide ligustilide butene-NBP |
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