| 黄芪多糖含量测定的方法学研究 |
| |
| 引用本文： | 李孝栋,陈景山,陈峰,张小如.黄芪多糖含量测定的方法学研究[J].世界科学技术,2006,8(2):35-37. |
| |
| 作者姓名： | 李孝栋 陈景山 陈峰 张小如 |
| |
| 作者单位： | 福建中医学院药学系药剂教研室,福州350003 |
| |
| 摘 要： | 本文采用紫外分光光度法测定黄芪中多糖的含量,通过对多糖部位的水溶部分、水不溶部分及两部分混合物的多糖含量测定,表明水溶部分与水不溶部分总多糖的含量和两部分混合物的多糖含量接近,结合多糖特性和Molish反应的阳性现象,提出用沸水代替冷水对黄芪多糖有效部位萃提的方法更具准确性.本研究结果证明苯酚-硫酸法也可用于测定水不溶多糖.
|
| 关 键 词： | 黄芪多糖 紫外分光光度法 含量测定 |
| 收稿时间： | 2005-10-27 |
| 修稿时间： | 2005-12-14 |
Study on Methodology for Determination of polysaccharides of Astragalus Membranaceus |
| |
| Li Xiaodong,Chen Jinshan,Chen Feng,Zhang Xiaoru.Study on Methodology for Determination of polysaccharides of Astragalus Membranaceus[J].World Science and Technology(Modernization of Traditional Chinese Medicine),2006,8(2):35-37. |
| |
| Authors: | Li Xiaodong Chen Jinshan Chen Feng Zhang Xiaoru |
| |
| Institution: | laboratory of Pharmac e utic s,Departme nt of Mate ria Medic a, Fujian Institute of Traditional Chinese Medicine,Fuzhou 350030 |
| |
| Abstract: | In this article the cotent of polysaccharides of Astragalus membranaceus is determined by ultraviolet spectrophotometry.And it is showed through the determination of saccharides of both the water-soluble and water-insoluble parts as well as the mixture of them with saccharides of Astragalus membranaceus that the total content of saccharides of the water-soluble and water-insoluble parts is close to that of their mixture and it is suggested that there must be more accuracy if saccharides of the effective parts of this plant is extracted with boiling water instead of cold water in consideration of the characteristics of saccharides and the positive reaction of Molish. The result of the study presented in this article proves that water-insoluble saccharides can be also determined by the method of phenol-sulfuric acid. |
| |
| Keywords: | poly saccharides of Astragalus membranceus uhraviolet spectrophotometry determination of content |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
