| 分光光度法对肿瘤特异生长因子的灵敏度及检测极限 |
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| 引用本文： | 黄韵竹,黄铁生,张信辉.分光光度法对肿瘤特异生长因子的灵敏度及检测极限[J].贵阳医学院学报,2003,28(5):409-410,413. |
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| 作者姓名： | 黄韵竹 黄铁生 张信辉 |
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| 作者单位： | 1. 贵阳医学院附院,生化科,贵州,贵阳,550004
2. 贵阳医学院,医学检验系,贵州,贵阳,550004 |
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| 摘 要： | 目的：用721可见分光光度法对随机混合血清的肿瘤特异生长因子(TSGF)值进行测定。考察该方法的灵敏度及检测极限。方法：严格按福建新大陆生物技术有限公司开发的TSGF联合检测试剂盒使用说明书进行。该试剂在特定条件下，与血清中的多种肿瘤相关物质产生显色反应。结果：721可见分光光度法的灵敏度为k=0．00586，检测极限为1．52U／ml。结论：721分光光度法对ISGF有较高的灵敏度和较理想的检测极限。该方法所需设备简单，操作简便快速，是基层医院及预防中心对肿瘤进行普查及筛选的理想方法，可推广应用。
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| 关 键 词： | 分光光度法 肿瘤特异生长因子 灵敏度 检测极限法 |
| 文章编号： | 1000-2707(2003)05-0409-02 |
The Sensitivity and Test Extremity of Detection of Tumor Specific Growth Factor with Visible Spectrophotometer |
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| HUANG Yun-zhu,HUANG Tie-sheng,ZHANG Xin-hui.The Sensitivity and Test Extremity of Detection of Tumor Specific Growth Factor with Visible Spectrophotometer[J].Journal of Guiyang Medical College,2003,28(5):409-410,413. |
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| Authors: | HUANG Yun-zhu HUANG Tie-sheng ZHANG Xin-hui |
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| Abstract: | Objective: To detect tumor specific growth factor (TSGF) in randomly mixed serum with 721 visible spectrophotometer, and to investigate the sensitivity and test extremity of this method. Methods; TSGF test reagent kit developed by Fujian New Continent Biology Technology Co. Ltd. was used. Results: The sensitivity of this method was K = 0. 005 86 and test extremity was 1. 52 U/ml. Conclusion: 721 visible spectrophotometer method has relatively high sensitivity and more ideal test extremity. The apparatus needed is simple and handy. So it is an ideal method for general investigation and screening of tumor in grassroot hospitals and precautionary centers. |
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| Keywords: | spectrophotometry tumor specific growth factor sensitivity extremity of test |
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