| 流式细胞术检测红细胞相关抗体及临床应用 |
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| 引用本文： | 钟明华,廖军鲜,龙红,许勇钢,杨晓红.流式细胞术检测红细胞相关抗体及临床应用[J].中日友好医院学报,2001,15(1):3-6. |
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| 作者姓名： | 钟明华 廖军鲜 龙红 许勇钢 杨晓红 |
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| 作者单位： | 1. 中日友好医院血液科,
2. 中国中医研究院西苑医院血液科, |
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| 摘 要： | 目的：建立流式细胞术（FCM）检测红细胞相关抗体(EAIg)的方法，以用于EAIg的检测，定量和分型。方法：利用流式细胞仪和抗人IgG,IgA,IgM单克隆抗体检测EAIg及亚型。结果：免疫性溶血贫血（IHA）患者IgG( )红细胞百分率显著高于正常组（P＜0．05），IgA,IgM阳性率也均达20．7％，本法结合单特异性抗C3血清可对IHA行进一步免疫学分型。结论：FCM对EAIg进行定测定及分型，方法灵敏、结果客观、对IHA的确诊具有较大价值。
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| 关 键 词： | 溶血性贫血 自身免疫性 流式细胞术 检测 |
| 文章编号： | 1001-0025(2001)01-0003-04 |
| 修稿时间： | 2001年1月15日 |
Detection of erythrocyte associated immunoglobulin by flow cytometry |
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| ZHONG Ming hua,LIAO Jun xian,LONG Hong,et al.Detection of erythrocyte associated immunoglobulin by flow cytometry[J].Journal of China-Japan Friendship Hospital,2001,15(1):3-6. |
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| Authors: | ZHONG Ming hua LIAO Jun xian LONG Hong et al |
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| Abstract: | Objective:To establish a flow cytometry (FCM) method for detection and immunophenotyping of erythrocyte associated immunoglobulin(EAIg).Methods:Flow cytometre and monoclonal antibodies were used to detect and analyze EAIg and their subsets.Results:The percentage of IgG positive red blood cell in immune patients with hemolytic anemia(IHA)was significantly higher than that of control group( P <0 05).IgA and IgM positive rates were both 20 7%.In IHA patients,immunophenotyping were performed by FCM.Conclusion:This method is not only sensitive,rapid and proper,but also show us the subsets of autoantibodies.It is helpful in the diagnosis of IHA. |
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| Keywords: | anemia hemolytic autoimmune flow cytometry |
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