全文获取类型
收费全文 | 1461篇 |
免费 | 103篇 |
国内免费 | 17篇 |
专业分类
儿科学 | 226篇 |
妇产科学 | 33篇 |
基础医学 | 151篇 |
口腔科学 | 5篇 |
临床医学 | 252篇 |
内科学 | 335篇 |
皮肤病学 | 12篇 |
神经病学 | 19篇 |
特种医学 | 9篇 |
外科学 | 118篇 |
综合类 | 163篇 |
预防医学 | 74篇 |
眼科学 | 4篇 |
药学 | 119篇 |
中国医学 | 21篇 |
肿瘤学 | 40篇 |
出版年
2023年 | 25篇 |
2022年 | 45篇 |
2021年 | 67篇 |
2020年 | 52篇 |
2019年 | 34篇 |
2018年 | 49篇 |
2017年 | 46篇 |
2016年 | 48篇 |
2015年 | 64篇 |
2014年 | 92篇 |
2013年 | 94篇 |
2012年 | 63篇 |
2011年 | 86篇 |
2010年 | 57篇 |
2009年 | 49篇 |
2008年 | 57篇 |
2007年 | 54篇 |
2006年 | 52篇 |
2005年 | 44篇 |
2004年 | 49篇 |
2003年 | 29篇 |
2002年 | 29篇 |
2001年 | 29篇 |
2000年 | 27篇 |
1999年 | 29篇 |
1998年 | 16篇 |
1997年 | 20篇 |
1996年 | 21篇 |
1995年 | 18篇 |
1994年 | 15篇 |
1993年 | 24篇 |
1992年 | 9篇 |
1991年 | 14篇 |
1990年 | 14篇 |
1989年 | 12篇 |
1988年 | 12篇 |
1987年 | 9篇 |
1986年 | 10篇 |
1985年 | 17篇 |
1984年 | 21篇 |
1983年 | 12篇 |
1982年 | 15篇 |
1981年 | 13篇 |
1980年 | 6篇 |
1979年 | 5篇 |
1977年 | 5篇 |
1976年 | 9篇 |
1975年 | 4篇 |
1973年 | 2篇 |
1969年 | 3篇 |
排序方式: 共有1581条查询结果,搜索用时 15 毫秒
1.
Rh血型不合新生儿溶血病检测方法及应用 总被引:3,自引:0,他引:3
产前检测Rh,D因子及抗人球蛋白(coombs)试验是必要的。测定Rh,D因子及抗D滴度使用木瓜酶方法。通过对11261例孕妇常规检查Rh,D因子,发现D阴性74例。Rh,D阴性妇女占6.5‰。22例Rh,D阴性的孕妇所分娩的新生儿均为Rh,D阳性。其中2例孕妇血清抗D滴度为1∶32,病情严重,宫内输血无效,胎死宫内。初产妇13例,占59%。活产20例,存活率90%。Rh因子及抗人球蛋白试验方法简便、易行,一般医院均可进行。对有流产史、输血史的孕妇检查Rh因子是十分必要的。在有条件的医院,对Rh,D阴性的产妇分娩Rh,D阳性的新生儿之后,产妇应预防性注射抗D免疫球蛋白 相似文献
2.
Percutaneous transluminal coronary angioplasty in a patient with paroxysmal nocturnal hemoglobinuria
Ping-Ching Fong Yau-Ting Tai Chu-Pak Lau John Li Florence Hiu-Yee Yap Raymond Liang Albert Kwok-Wai Lie 《Catheterization and cardiovascular interventions》1992,25(2):144-147
Paroxysmal nocturnal hemoglobinuria (PNH) is an acquired chronic hemolytic anemia associated with an unusual susceptibility to hemolytic crisis, infection, and venous thrombosis which would be aggravated by a number of factors including surgery. We report a case of PNH undergoing percutaneous transluminal coronary angioplasty and discuss the corresponding perioperative management. 相似文献
3.
Mayumi Ujihara Sumiko Hamanaka Sachie Matsuda Fumitaka Numa Hiroshi Kato 《The Journal of dermatology》1994,21(1):56-58
A 76-year-old female was admitted with many bullae and erythema on her trunk and extremities. A biopsy specimen showed significant intercellular edema in the lower epidermis and eosinophilic infiltration into the dermis and the epidermis. Immunofluorescent staining revealed the deposition of IgG in the intercellular area of her prickle cells. From these histologic findings and the typical clinical features, we diagnosed her as having pemphigus vulgaris. Examination of her blood revealed that she also suffered from autoimmune hemolytic anemia. Despite intensive treatment with prednisolone, she finally died. This case is of interest because of its rarity and the TNFα detected significantly in the blister fluid of this patient. 相似文献
4.
5.
J D Tissot F Clément J A Schifferli P C Frei D F Hochstrasser P Schneider 《American journal of hematology》1992,40(3):171-175
High-resolution two-dimensional gel electrophoresis (2-DGE) was used to analyse plasma samples and partially purified cold agglutinins (CA) obtained from two selected patients. Both presented an acute hemolytic anemia with CA of high thermal amplitude, normal immunoglobulin levels, no detectable paraproteinemia, and no clinical evidence of a malignant B-cell disorder. The electrophoretograms of their plasma showed evident alternations of the "normal" protein profile, which were directly related to hemolysis (absence of the spots of haptoglobin and in one case of those of hemopexin), but no monoclonal gammopathy. The electrophoretograms of their purified CA revealed two clearly different spot patterns respectively corresponding to a monoclonal IgM and to polyclonal IgM. These results show that the clonality of CA associated with hemolytic anemia can be easily determined by 2-DGE. This technique may be very useful to discriminate chronic cold agglutinin disease in the early phase from "parainfectious" CA. 相似文献
6.
Moallem HJ Taningo G Jiang CK Hirschhorn R Fikrig S 《Clinical immunology (Orlando, Fla.)》2002,105(1):75-80
Purine nucleoside phosphorylase (PNP) deficiency results in an autosomal recessive immunodeficiency disease characterized by initial involvement of cellular immunity and neurological manifestations with subsequent abnormalities of humoral immunity. The initial presentation and clinical course has varied widely in the relatively few published cases. The molecular basis has been reported in only 10 patients, precluding evaluation of phenotype-genotype relationships. We now report clinical, immunologic, and molecular findings in a new case of relatively early onset that emphasizes hypotonia and developmental delay as early manifestations. The patient carried two novel missense mutations (Gly56A1a and Val217Ile) on the same allele in apparent homozygosity. Expression of each of the mutant enzymes in vitro demonstrated that the Gly156A1a mutation abolished enzyme activity while the Val217Ile mutation was without obvious effect and is therefore a normal variant. Such "normal" polymorphisms might be associated with a variable response to the immunosuppressive PNP inhibitors currently in clinical trials. 相似文献
7.
Dr. G. Sieber F. Herrmann B. Enders H. Rühl 《Journal of molecular medicine (Berlin, Germany)》1982,60(20):1303-1309
Summary Using a reverse hemolytic protein A plaque assay, spontaneous and pokeweed mitogen (PWM)-induced immunoglobulin (Ig) secretion was determined in peripheral blood from 22 patients with B1-chronic lymphocytic leukemia (CLL), one patient with B2-CLL, and one patient with suppressor T-CLL. Diagnoses were established by cytological and histological criteria as well as several marker analyses. Lymphocytes from B1- and B2-CLL patients were unable to secrete Ig either spontaneously or after PWM stimulation. In T-CLL lymphocytes, spontaneous Ig secretion was suppressed very probably by the OKT-8-positive leukemic population, since, after cultivation with PWM, a normal Ig secretion could be demonstrated which was paralleled by a decrease in the OKT-8-positive cells. Cocultivation experiments with freshly isolated, unseparated lymphocytes from normal subjects and lymphocytes from patients were of no informational value, since isolated normal B-cells alone already showed a high rate of Ig secretion. However, coculture experiments with separated subpopulations after PWM stimulation revealed an intrinsic B-cell defect in lymphocytes from B1-CLL patients, whereas their T-lymphocytes were found to be normal helper cells.
Abbreviations CLL Chronic lymphocytic leukemia - PWM Pokeweed mitogen - ISC Immunoglobulin-secreting cells - Ig Immunoglobulin(s) Supported by the Deutsche Forschungsgemeinschaft (Ru 215/2) 相似文献
Abbreviations CLL Chronic lymphocytic leukemia - PWM Pokeweed mitogen - ISC Immunoglobulin-secreting cells - Ig Immunoglobulin(s) Supported by the Deutsche Forschungsgemeinschaft (Ru 215/2) 相似文献
8.
9.
Inge Van de Walle Karen Silence Kevin Budding Liesbeth Van de Ven Kim Dijkxhoorn Elisabeth de Zeeuw Cafer Yildiz Sofie Gabriels Jean-Michel Percier Johanna Wildemann Jan Meeldijk Peter J. Simons Louis Boon Linda Cox Rob Holgate Rolf Urbanus Henny G. Otten Jeanette H.W. Leusen Peter Boross 《The Journal of allergy and clinical immunology》2021,147(4):1420-1429.e7
10.
G L Sussman R P Harvey A L Schocket 《The Journal of allergy and clinical immunology》1982,70(5):337-342
Delayed pressure urticaria (DPU) is a poorly understood syndrome. We describe 17 patients with DPU. Chronic urticaria was present in 94%. All had negative challenges for immediate demographism and cold urticaria. DPU was induced with a pressure challenge on the shoulder of 15 pounds for 15 min. Average onset of pressure lesions after challenge was 6.5. Lesions were painful, not pruritic, peaked at 9 hr, and disappeared by 24 to 48 hr. Fever, chills, and/or arthralgias occurred in 78%. Positive laboratory abnormalities included leukocytosis in 20% and elevated erythrocyte sedimentation rate in 37.5%. Skin biopsies of lesions showed perivascular round cell infiltrates and negative immunofluorescence. Urticaria responded to antihistamines, but not aspirin, in 100% of patients, while pressure lesions improved with nonsteroidal anti-inflammatory drugs (NSAID), but not antihistamines, in 80% of patients. Both urticaria and DPU were controlled with prednisone, which was necessary in 87.5% of patients. A severe nonremitting course was noted in 7%, 40% had a moderate remitting course requiring intermittent prednisone, and 53% had a mild remitting disease requiring no medication or antihistamines and/or NSAID only. We conclude that DPU is more common than previously appreciated and likely involves mediators other than histamine, possibly the prostaglandin system. 相似文献