保守治疗牙源性角化囊肿的初步探讨

目的:探讨保守治疗牙源性角化囊肿的可行性和临床意义。方法:对我院近几年收治的囊肿较大、多发或不愿作根治术的12名患者,行彻底刮治术加开窗术的保守治疗,术后佩带塞治器,观察其疗效和复发率。结果:术后0.5～1年X线显示9例原病变区有大量新生骨质沉积,3例无明显疗效,有效率为75%,随访1～3年,复发4例,复发率为33.3%。结论:牙源性角化囊肿保守治疗有一定临床疗效,但尚需降低复发率。     

护理本科毕业生核心能力综合评价指标体系的研究

Objective To probe into the structure of core competences of baccalaureate nursing graduates,structuring a system of indexes evaluation of baccalaureate nursing graduates' core competences.Methods Adopting Delphi method and AHP method to evaluate the items,layer structure,weights,connotation of the indexes system.All data were collected and statistically treated.Results After two rounds of consultations,built an index evaluation system of baccalaureate nursing graduates' core competences.Individual Authority Coefficient of all experts are 0.775～1.0;the level of concordance of experts' opinion about index is above of 91.9%,the coefficient of concordance is 0.84,CI or CR of the logic concordance of all indexs is less than 0.1.Conclusions The index evaluation system of baccalaureate nursing graduates' core eompetenees is built after two rounds of consultation,the experts have high authority and concordance,CI or CR of the logic concordance of all indexs is less than 0.1.the evaluation sysem is scientific and reliable.     

Cystadenoma in a seminal vesicle is cured by laparoscopic ablation

Dear Editor,
We present here a case report of a benign seminal vesicle cystadenoma treated with laparoscopic ablation in a 31-year-old man. The clinical presentation, gross appearance, microscopic characteristics, immunohistochemical and ultrastructural findings of this uncommon tumour are discussed.     

咖啡因对辐射诱导细胞凋亡和G2／M期阻滞的影响

用形态学,DNA电泳和流式细胞仪等方法观察γ射线诱发BAF3细胞G_2/M期阻滞和凋亡的作用,以及咖啡因对其的影响。结果显示,5Gyγ射线照射后6小时引起细胞G_2/M期阻滞,G_2/M期比例为49.10％,12小时升至52.00％,无细胞凋亡发生。照前分别加入1mmol/L及5mmol/L咖啡因,照后12小时G_2/M阻滞程度降低,比例降至39.42％和15.49％,形态学检测细胞凋亡比例为8.33±1.53％和18.33±1.76％,流式细胞仪检测细胞凋亡比例为18.78％和43.81％,DNA电泳可见细胞凋亡特有的梯状图谱。结论提示,G_2/M期阻滞抑制能促进辐射诱导的细胞凋亡。     

Adipose-derived stem cells promote tumor initiation and accelerate tumor growth by interleukin-6 production

Adipose-derived stem cells (ADSCs) are multipotent cells that have attracted much recent attention. Here, we show that ADSCs enhance sphere formation and in vivo tumor initiation of breast and colon cancer cells. In co-culture, ADSCs induced several stem cell markers in cancer cells. ADSCs also accelerated tumor growth. Interaction of ADSCs and cancer cells stimulated secretion of interlukin-6 in ADSCs, which in turn acted in a paracrine manner on cancer cells to enhance their malignant properties. Interleukin-6 regulated stem cell-related genes and activated JAK2/STAT3 in cancer cells. We suggest that ADSCs may enhance tumor initiation and promotion.     

Lipid overaccumulation and drastic insulin resistance in adult catch-up growth rats induced by nutrition promotion after undernutrition

This study was designed to explore the metabolic changes resulting from catch-up growth in adult (CUGA) induced by varying degrees of nutrition promotion after undernutrition and to confirm whether these changes are transient or not. The CUGA models were developed on rats refed on intakes of normal chow or high-fat diet after a period of caloric restriction. The growth of the rats measured by body weight and length stagnated during caloric restriction and then rapidly accelerated following refeeding. Catch-up growth in adult resulted in an increase in intramuscular and intrahepatic lipid content, visceral fat deposition, and insulin resistance, which is consistent with a transient rise in food efficiency during the early stage of refeeding. In addition, ectopic lipid deposition, visceral fat accumulation, and insulin resistance were more severe in rats refed the high-fat diet than rats refed the normal chow. These findings suggest that CUGA induced by rapid nutrition promotion could result in persistent lipid overaccumulation (increased visceral fat and ectopic lipid deposition) and drastic systemic insulin resistance. The effects of CUGA on metabolic characteristics are dependent on the type of diet that is used for refeeding, especially on the amount of fat intake.     

脊髓造影辅助经皮椎板间孔内窥镜下髓核摘除术治疗L5/S1椎间盘突出症

目的:评估术中脊髓造影辅助经皮椎板间孔入路内窥镜下髓核摘除术治疗L<,5>/S<,1>椎间盘突出症的临床价值.方法:选择确诊为L5/S1单节段腰椎间盘突出症患者共40例,采用随机数字表的方法将患者分为A组和B组,每组各20例.A组在脊髓造影辅助下行经皮椎板间孔入路内窥镜下髓核摘除术,B组不实施脊髓造影操作,其它方法同A组.记录两组穿刺到目标椎间盘所需时间,一次性穿刺成功率,穿刺过程中神经根刺激症状和神经根、硬膜囊损伤发生率,脊髓造影相关的并发症.两组病例均随访6～12个月.采用疼痛VAS评分、Oswestry功能障碍指数(ODI)、改良的MacNab分级标准为疗效观察指标.结果:A组穿刺到目标椎间盘时间为9.67±1.45min,B组14.40±2.35min(P<0.01);A组一次性穿刺成功率95%(19/20例),B组60%(12/20例)(P<0.05);A组穿刺过程中神经根刺激症状发生率15%(3/20例),B组55%(11/20例)(P<0.05);A组无神经根和硬膜囊损伤发生,B组神经根和硬膜囊损伤各1例.按照改良MacNab疗效评定标准,A组优良率85%,B组优良率80%,两组无显著性差异(P>0.05).两组患者术后VAS和ODI评分较术前明显改善(P<0.05),但两组间比较无差异(P>0.05).A组中未见和脊髓造影相关并发症发生.结论:脊髓造影辅助经皮椎板间孔入路内窥镜下髓核摘除术治疗L<,5>/S<,1>椎间盘突出症的优点是减少目标椎间盘的穿刺时间,提高一次性穿刺成功率,降低并发症的发生率.     

Effects of Folic Acid on Cardiac Myocyte Apoptosis in Rats with Streptozotocin-induced Diabetes Mellitus

BACKGROUNDS: The effect of folic acid on cardiac myocyte apoptosis secondary to diabetes is unknown. METHODS: Diabetic rats were divided into diabetic control (DC, n = 11), low-dose (LDF, 0.4 mg/kg/day, n = 12) and high-dose (HDF, 1.2 mg/kg/day, n = 12) folic acid groups. Non-diabetic rats (n = 11) were used as the normal control (NC). RESULTS: After 11 weeks of treatment, compared with the NC group, the DC group showed a reduced blood levels of reactive oxygen species (ROS, P < 0.01). The rate of cardiac myocyte apoptosis in the diabetic control group was also greater than in the non-diabetic control group (P < 0.01). In folic acid-treated rats, the blood levels of ROS was higher than in the diabetic control group (P < 0.05). There was a dose-dependent reduction in the rate of cardiac myocyte apoptosis in the folic acid groups (P < 0.01), and this was accompanied by an increased level of anti-apoptotic protein Bcl-2 and decreased level of pro-apoptotic protein Bax and Fas (P < 0.01). CONCLUSIONS: Dietary folic acid supplementation diminishes the cardiac myocyte apoptosis in streptozotocin-induced diabetes. The apoptosis suppression is accompanied by an increase in the expression of Bcl-2 and a decrease in Bax and Fas.     

一种改良的胚胎大鼠颞叶海马区神经元的培养方法

目的:探索胚胎大鼠颞叶海马区神经元的改良培养方法。方法:在无血清培养基础上,采用改良的分离纯化法获取单细胞悬液,接种后在不同阶段通过加入不同配方的培养基进行培养,并进行免疫组化鉴定。结果:用该方法培养的颞叶海马区神经元细胞存活率高,生长状态良好,且βⅢ-tublin免疫染色为阳性,神经元细胞纯度可达90%以上。结论:改良分离、结合分阶段的不同培养条件是一种简单、高效的颞叶海马区神经元的纯化培养方法。