微课在医学高专生物化学实验教学中的应用效果观察

目的分析微课在医学高专生物化实验教学中的应用效果。方法将我省某高专三年制医学专业1班、2班、3班、4班学生作为研究对象,研究组(1班与2班合班,共122人)接受微课式实验教学,对照组(3班与4班合班,共120人)接受实传统实验式教学,比较两组学生成绩,并给予问卷调查学生对此次教学方式的意见。结果研究组考试成绩为(92.6±1.3)分,高于对照组的(80.4±1.1)分,数据差异有统计学意义(P0.05)。比较两组学生对此次教学模式满意度,研究组总体满意度为90.98%,高于对照组的73.33%,数据差异有统计学意义(P0.05)。结论微课在医学高专生物学实验教学中应用效果突出,可提升学生学习兴趣,改善教学质量。     

失眠吃点龙眼枸杞粥

<正>有位男士前一段时间晕倒在工作岗位上,原因是长期失眠导致身体免疫力下降,诱发了心脏病。这位男士失眠不止一天两天了,之前他尝试了许多办法,甚至还花过很多钱买了一个据说能促进睡眠的椅子,却都收效甚微。看过他的情况后我发现,他失眠主要是因为血虚,心血     

红景天多糖对精原干细胞体外增殖的影响

BACKGROUND: To establish a rapid and effective method to obtain sufficient spermatogonial stem cells that can meet the clinical need is urgent to be solved in the spermatogonial stem cell transplantation. OBJECTIVE: To study the effect of rhodiola polysaccharide on the proliferation of spermatogonial stem cells in vitro. METHODS: Under sterile conditions, spermatogonial stem cells and Sertoli cells were isolated from the testis of mice, and spermatogonial stem cells were seeded onto the feed layer of Sertoli cells. Then, the co-cultured cells were assigned into experimental group 1 (simple cell culture medium), experimental group 2 (cell culture medium containing 150 mg/L rhodiola polysaccharide) and experimental group 3 (cell culture medium containing 150 mg/L rhodiola polysaccharide, 1 U/L leukemia inhibitory factor and 10 μg/L glial cell line-derived neurotrophic factor). After 7 days of co-culture, flow cytometry was used to detect cell proliferation in vitro, and cell viability and positive expression of GFRa-1, Thy-1 and C-kit were calculated. RESULTS AND CONCLUSION: After 7 days of co-culture, the cells grew rapidly and presented with colony and thyrsiform growth, and the number of cell masses increased significantly, all of which were in line with the proliferative features of spermatogonial stem cells. The GFRa-1, Thy-1 and C-kit proteins were expressed in the cell membrane and cytoplasm, mainly in the cell membrane. The viability of spermatogonial stem cells and positive expression of GFRa-1 and Thy-1 were ranked as follows: experimental group 3 > experimental group 2 > experimental group 1, and there were significant differences between groups (P < 0.05). The positive expression of C-kit had no difference between experimental groups 1 and 2, but it was significantly higher in the experimental group 3 than the other two groups (P < 0.05). These findings indicate that rhodiola polysaccharide used alone or combined with leukemia inhibitory factor and glial cell line-derived neurotrophic factor can enhance the proliferative ability of spermatogonial stem cells in vitro.  中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程     

心肝肾三脏在阴茎勃起机制中的功用

心、肝、肾三脏在阴茎勃起功能障碍发病中,常相互影响,共同致病。因郁而病或因病而郁,恶性循环,以致肝气郁滞,心神不宁,气血失调,血不能注,宗筋不振;思想无穷,耗神过度,久病失养,皆可导致心气不足,心阴耗损,君火不能下济肾命,肾中精气不能上奉心君,终至心肾两虚,心肾不交,病发阳痿;肝肾同源,同寄相火,禀赋不足、疾病日久可导致肾气亏虚、肾精耗损,从而发生性欲减退、宗筋弛缓,酿成阳痿。     

塞来昔布纳米混悬剂的制备及大鼠体内药动学研究

摘 要 目的： 制备塞来昔布纳米混悬剂（CXB NSs）,并考察大鼠灌胃给药后体内药动学特征。方法: 采用反溶剂沉淀 高压均质法制备CXB-NSs,并考察其粒度分布,多聚分散系数和Zeta电位。将12只Wistar大鼠随机分为CXB NSs组和CXB混悬液组,灌胃给药剂量均为100 mg·kg^-1,采用高效液相色谱法测定大鼠血浆中的CXB浓度,用3P97软件计算相应的药动学参数。结果: CXB NSs平均粒径为(442.5±61.9) nm,多聚分散系数为0.312±0.057,Zeta电位为(-31.6±3.9) mV。CXB NSs和CXB混悬液在大鼠体内的AUC(0-t)分别为(5.13±0.77)和(13.51±3.18) mg·L^-1·h;t1/2分别为(12.31±1.91)和(12.73±1.83) h;Tmax 分别为(2.48±0.37)和(1.41±0.27) h;Cmax分别为(0.94±0.31)和(2.38±0.25) mg·L^-1。结论:CXB NSs能显著提高药物在大鼠体内的生物利用度。     

阳痿肾阳虚证同证异治浅析

阳痿多辨证为肾阳虚证,一般治疗以补肾为主,但不同疾病所致阳痿疗效不同,故阳痿当同证异治,根据不同疾病导致的阳痿,采用不同治法,不能局限于同证同治,方可获效.     

4种尸食性蝇类卵的表面超微结构

:【目的】明确尸食性蝇类卵的形态 ,以便于在医学和法医学中的应用。【方法】用扫描电镜机观察杭州地区 4种尸食性蝇类卵的形态。【结果】丝光绿蝇、大头金蝇、巨尾阿丽蝇和厩腐蝇卵的超微结构形态各异 ,卵的大小、表面形态、中区、孵出线、卵孔和垂柱皆为重要的鉴别特征。【结论】通过卵壳超微结构可区分常见尸食性蝇类。     

王久源教授辨治慢性前列腺炎临床经验

王久源教授从事临床男科工作多年，对治疗慢性前列腺炎有独到见解，本对其治疗慢性前列腺炎的临床思维进行系统总结，以飨读。     

精液迟缓液化症的诊疗现状

按WHO规定：新采集的精液标本在室温(25℃)60min内发生精液液化，若超过60min仍未液化，则称为精液迟缓液化症。Burge于1954年首先提出精液迟缓液化症与男性不育有关，后许多学者对此进行了研究。国外报道男性不育患者中精液迟缓液化为唯一指标者占11．8％，国内报道占7％～9％。随着对此症研究的深入，新的检测和治疗方法不断出现，并在临床中得到肯定。现将其归纳总结如下。     

开窗支架技术治疗复杂主动脉弓降部病变的早期疗效

目的 探讨单独开窗、开窗联合分支支架技术治疗复杂胸主动脉弓降部病变的早期效果.方法 选取2019年1月至2020年5月安徽医科大学第一附属医院收治的18例复杂主动脉弓降部病变患者,10例采用单独体外开窗技术重建主动脉弓上分支动脉,8例为体内开窗联合分支支架技术重建弓上分支动脉.观察术后破口封闭、降主动脉重塑及分支动脉血运情况.术后每半年门诊随访1次,随访内容包括体格检查、化验和影像检查.结果 18例患者手术顺利,无死亡,无围术期严重并发症,随访率100％.所有患者无院外胸痛、腹痛及晕厥主诉.术后6个月全部患者经CTA证实,主体支架形态良好,分支动脉通畅,无内漏、移位.结论 开窗联合分支支架技术能为部分复杂胸主动脉弓降部病变实现微创治疗,近期疗效确切.