苦寒药与生物钟基因的关系研究进展＊

苦寒类中药善于清热燥湿以改善机体内环境稳态，目前在临床上被广泛应用于糖尿病、肥胖、炎症、菌群失调等慢性或代谢性疾病，而这些疾病往往都伴随着机体生物钟系统的失衡。改善生物钟失衡可能是苦寒药治疗这些疾病的作用机制之一，同时，在生物钟的调控下，机体新陈代谢及内分泌等行为的节律性波动也可能影响苦寒药的药效。既往对苦寒药在临床应用上的研究大部分集中于胃肠动力及激素等基础研究上，从生物钟基因的角度鲜有见到深入的研究与阐述。因此，本文从临床常用苦寒药对生物钟基因的影响及生物钟基因对苦寒药作用的影响两个方面探讨苦寒药的应用，同时分析二者之间的关系和归纳苦寒药影响生物钟基因共同的物质基础，以期为苦寒药作用多靶点以及指导临床合理用药和中药药性物质基础研究提供参考。     

手术室医辅人员任务智能分配的抢单模式管理

目的研发适用于手术室医辅人员任务分配管理的智能软件信息系统,并探讨其运行效果。方法将2018年3月试运行抢单信息系统的509台次手术设为实验组,对医辅人员运用抢单模式进行管理;对同期未实施的486台次同类手术设为对照组,运用常规管理模式;比较两组的运行效果。结果实验组应答时间和完成任务时间显著短于对照组,护士满意率显著提升(均P0.01)。结论抢单信息系统精准记录了医辅人员的工作量和时间节点,提高了工作效率和护理人员满意率,促进了后勤医辅人员的科学化、精细化管理。     

Wnt/β-catenin links oxidative stress to podocyte injury and proteinuria

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教育信息化2.0背景下网络教育的现状及对策思考——以陕西省为例

《教育信息化2.0行动计划》发布以来,全国各地积极推动教育信息化的发展,网络教育变得越来越普及。虽然网络教育在发展的过程中也越来越完善,但是还存在着一些问题。文章通过分析陕西省网络教育的现状,总结目前存在的问题并提出相应的对策,有助于更好地发展和完善网络教育,为教育信息化的发展提供借鉴。     

药品审评检查机构质量管理体系建设实践探索

目的:贯彻国务院强化质量管理、落实全国药监部门完善自身质量管理体系工作部署,梳理山东省药品审评检查机构质量管理体系建设情况,总结经验、查找不足,加强审评检查质量管理。方法:调研药品审评检查机构导入质量管理体系的现实意义,剖析山东省药品审评检查机构质量管理体系建设的实践经验,思考存在的问题。结果:行之有效的质量管理体系对药品审评检查机构规范化建设和药品审评检查发展起到了积极的推动作用。结论:药品审评检查机构推进质量管理体系建设,对规范自身建设、提高工作质量、降低业务风险、服务医药产业发展具有重要作用,也是必然要求,药品审评检查机构质量管理体系需要持续改进,逐渐与国际接轨。     

针刺联合艾灸对慢性盆腔炎患者炎性反应指标及中医症候积分的影响

目的:探究针刺联合艾灸对气滞血瘀型慢性盆腔炎性患者炎性反应指标及中医症候积分的影响。方法:选取2017年3月至2019年3月安徽中医药大学第二附属医院收治的气滞血瘀型慢性盆腔炎性患者60例作为研究对象,将其按照随机数字表法分为观察组和对照组,每组30例。2组患者均行常规抗炎治疗,对照组在此基础上加用针刺治疗,观察组在对照组治疗基础上加用艾灸治疗,对比治疗前后2组白细胞计数、中医证候积分及不良反应情况。结果:观察组的临床治疗有效率明显优于对照组(P<0.05);分组治疗后观察组中医症候积分均明显低于对照组及治疗前(P<0.05);治疗后观察组RBC明显低于对照组和治疗前(P<0.05);治疗期间观察组不良反应发生率低于对照组,但差异无统计学意义(P>0.05)。结论:针刺联合艾灸可显著提高慢性盆腔炎性的治疗效果,改善患者中医证候、减轻炎性反应,且治疗安全性高,值得临床推广应用。     

The effect of acetylation on the protein stability of BmApoLp‐III in the silkworm,Bombyx mori

Acetylation is an important, reversible posttranslational modification to a protein. In a previous study, we found that there were a large number of acetylated sites in various nutrient storage proteins of the silkworm haemolymph. In this study, we confirmed that acetylation can affect the stability of nutrient storage protein Bombyx mori apolipophorin‐III (BmApoLp‐III). First, the expression of BmApoLp‐III could be upregulated when BmN cells were treated with the deacetylase inhibitor panobinostat (LBH589); similarly, the expression was downregulated when the cells were treated with the acetylase inhibitor C646. Furthermore, the increase in acetylation by LBH589 could inhibit the degradation and improve the accumulation of BmApoLp‐III in BmN cells treated with cycloheximide and MG132 respectively. Moreover, we found that an increase in acetylation could decrease the ubiquitination of BmApoLp‐III and vice versa; therefore, we predicted that acetylation could improve the stability of BmApoLp‐III by competing for ubiquitination and inhibiting the protein degradation pathway mediated by ubiquitin. Additionally, BmApoLp‐III had an antiapoptosis function that increased after LBH589 treatment, which might have been due to the improved protein stability after acetylation. These results have laid the foundation for further study on the mechanism of acetylation in regulating the storage and utilization of silkworm nutrition.     

Aldehyde dehydrogenase 2 deficiency significantly exacerbates tert-butyl alcohol-induced toxicity in mice

Owing to the use of ethyl tert-butyl ether (ETBE) as a fuel additive, the possible adverse effects of ETBE exposure have become a public concern. Our previous study showed that ETBE-induced toxicity in aldehyde dehydrogenase 2 (Aldh2) gene knockout (KO) mice was caused by its primary metabolite acetaldehyde, which was toxic. However, it is unclear whether tert-butyl alcohol (TBA), another main metabolite of ETBE, plays a role in ETBE-induced toxicity. To investigate this relationship, we analyzed the changes of TBA concentrations in tissues after ETBE exposure, and then evaluated the toxicity after direct TBA treatment in both KO and wild-type (WT) mice. An exposure to 500 ppm ETBE via inhalation resulted in the formation of its three metabolites, TBA, 2-methyl-1,2-propanediol and ethanol, whose concentrations in the liver, brain, fat and testis of male KO mice were significantly higher than the corresponding concentrations observed in male WT mice. Direct treatment to TBA (20 mg/mL of drinking water) caused significant changes in relative organ weights and histopathology, and increased levels of genetic damages in both types of mice. These toxic effects were also seen in KO mice exposed to a lower concentration of TBA (5 mg/mL), which was associated with increased oxidative stress in serum (reduced glutathione and reduced glutathione/oxidized glutathione ratio decreased). Our findings indicate that ALDH2 is involved in the metabolism of ETBE and TBA, and ALDH2 deficiency could greatly increase the sensitivity to TBA-induced toxicity.     

腹腔镜下中间入路与侧方入路右半结肠癌根治术的术中、术后情况比较

目的:探讨腹腔镜下中间入路右半结肠癌根治术的临床疗效及并发症。方法:回顾性分析在2015年6月1日至2018年6月1日期间由中国人民解放军第967医院普外科收治的82例右半结肠癌患者临床资料，45例患者接受腹腔镜中间入路右半结肠癌根治术，为中间入路组，37例患者接受腹腔镜侧方入路右半结肠癌根治术，为侧方入路组；对比两组患者手术时间、术中出血量、清扫淋巴结数量、中转开腹率、术后肠功能恢复时间、术后住院时间及术后并发症等临床指标，评价分析腹腔镜下中间入路右半结肠癌根治术的优越性。结果:中间入路组手术时间、术中出血量、淋巴结清扫数量、中转开腹率与侧方入路组相比差异显著，均有统计学意义（P＜0.05）；但中间入路组在术后肠功能恢复时间、术后住院时间及术后并发症发生情况等方面与侧方入路组相比无统计学差异（P＞0.05）。结论:采用腹腔镜下中间入路右半结肠癌根治术能缩短手术时间、减少术中出血量、减少中转开腹率、增加清扫淋巴结个数，近期治疗效果满意，值得在临床中应用并推广。