升阳益胃汤对腹泻型肠易激综合征模型大鼠血钙基因相关肽及5-羟色胺的影响

目的：观察升阳益胃汤对腹泻型肠易激综合征（IBS）大鼠血浆钙基因相关肽（CGRP）、血清5-羟色胺（5-HT）的影响。方法取清洁级健康雄性SD大鼠60只,其中10只为空白对照组,60只采用束缚应激＋夹尾刺激法诱导腹泻型IBS大鼠模型,造模成功后随机分为模型对照组、得舒特组及升阳益胃汤低、中、高剂量组,各10只,观察大鼠稀便率,放免法检测大鼠血浆CGRP、血清5-HT含量。结果升阳益胃汤治疗后,大鼠稀便率下降,与模型组、得舒特组相比,差异有统计学意义（P〈0.05,P〈0.01）;模型组大鼠血清5-HT水平明显高于空白组,血浆CGRP水平则稍低于空白组（P〈0.05）;治疗后得舒特组、升阳益胃汤各剂量组血清5-HT均较模型组明显下降（P〈0.05）,而各组之间血浆CGRP水平差异无统计学意义（P〉0.05）。结论升阳益胃汤能明显减轻腹泻型IBS模型大鼠腹泻症状,减少腹泻型IBS模型大鼠血清5-HT的合成和分泌,但对血CGRP无明显影响。     

曾耀明 郑伟伟 余维微 陈余圣 林上助

Objective: To observe the level of serum CGRP and 5-hydroxytryptamine in animals model of irritable bowel syndrome treated with sheng yang yi wei decoction.Methods: 70 SPF SD rats were divided randomly into normal control group,model group,dicetel group,sheng yang yi wei decoction group(low dosage,moderate dosage,high dosage).The rat model of irritable bowel syndrome was made by binding stress and clasping their tails.group.Changes of diarrhea rate were observed in these groups．Radioimmunoassay was used to detect the level of CGRP and 5-HT in plasma．Results：The diarrhea rates in group were decreased after treatment,and had the statistics significance in comparison with model group and dicetel group(P<0.05)．The contents of 5-HT in plasma in model group were higher than those in normal control group,while the levels of CGRP in model group were slighly lower than those in normal control group(P<0.05).After treatment,the contents of 5-HT in plasma in dicetel group and sheng yang yi wei decoction group were markedly decreased than those in model group(P<0.05),while the levels of CGRP had no statistics significance among all groups(P>0.05).Conclusions: Sheng yang yi wei decoction can markly alleviate the symptom of diarrhea,inhibit 5-HT synthesis and secretion in animal model of irritable bowel syndrome,while it does not effect the levels of CGRP.     

龙惠珍应用益气祛风法治疗周围性面瘫经验

龙惠珍为副主任中医师、副教授、硕士生导师,其在30多年的工作实践中积累了丰富的临床和教学经验,擅长治疗内科常见病及疑难病.周围性面瘫又称特发性面神经麻痹,临床上以口眼内斜为主要表现,属于中医"口眼斜"的范畴,俗称"歪嘴巴"、"吊线风",目前西医临床治疗以肾上腺皮质激素、抗病毒药、维生素及神经营养药物为主,但副作用相对较大.龙惠珍老师运用益气祛风法治疗周围性面瘫,取得较满意的疗效,现将其经验介绍如下.