CEACAM1、CEACAM6在肿瘤中的作用研究进展

在恶性肿瘤的诊断中，癌胚抗原（ CEA ）是重要的指标之一，在人类部分肿瘤中均有明显的高表达，如结肠癌、乳腺癌、胃癌和肺癌等［1、2］，尤其是在结肠癌患者血清学的检测已用于临床，并被证实CEA表达的升高是结直肠癌复发的重要标志。目前发现CEA家族共有29个相关的成员，均分布在染色体19q13,2上。CEA家族成员均与黏附蛋白的功能相关，因此CEA家族又被学者们命名为癌胚抗原相关黏附分子。目前家族中人们主要关注的是 CEACAM1和 CEACAM6的作用。而人们在对肿瘤细胞生长、扩散和转移的研究中发现肿瘤的基因、蛋白表达失调，同时伴有较多的影响因素，并认为肿瘤进展过程中调节因子的功能多种多样，生物学行为非常复杂。在肿瘤发展过程中上述行为交织存在，关系密切，任何一种改变均可以引起相关的级联过程［3、4］。恶性肿瘤细胞经脉管播散又是其转移的重要途径。肿瘤血管生成已被证实在肿瘤发生、发展、转移中具有非常重要的作用，目前学者主要关注血管生成的影响因素。     

25-羟维生素D与认知障碍的研究进展

近几年对维生素D在维持人体健康上的作用的研究不断增加,越来越多的证据表明维生素D不仅对预防老年人骨质疏松症及骨折有明显益处,而且可能降低癌症、感染、自身免疫性疾病、心血管系统及神经系统疾病的发病率。目前研究指出维生素D具有神经保护作用,可以控制诱发认知障碍的促炎细胞因子,参与神经递质乙酰胆碱的合成,也与动脉粥样硬化、脑梗死、糖尿病及高血压病等有关。     

脑卒中预警信号 早知早防范

2019年,国际权威医学杂志《柳叶刀》有文章回顾分析了中国居民在1990年至2017年的十大死因,脑卒中是我国居民第一大死因。脑卒中是怎么回事脑卒中,俗称中风,分为缺血性脑卒中和出血性脑卒中,也就是大家常说的脑梗死和脑出血。脑梗死,通俗来讲就是血管堵了,脑组织缺血、缺氧性坏死;脑出血,则是指血管破了,脑组织因为出血而坏死。     

脂肪性肝病与胃食管反流病的相关性研究

目的评估脂肪性肝病(FLD)与胃食管反流病GERD之间的关系。方法选取2 000例受试者,采用较简便的胃食管反流病问卷(GerdQ)作为初筛GERD诊断标准,对入选者均进行腹部超声波等检查,分析FLD与GERD关系。结果对2 000例患者利用GerdQ评估GERD诊断情况,有176例(8.8%)诊断为GERD,女68人(38.64%),男108人(61.36%);脂肪肝患者760例,患病率38.0%,在脂肪肝组中,男女性GERD症状的GerdQ阳性率分别为13.77%和12.24%,两者无明显差异(P>0.05)。脂肪肝人群发生GERD症状的患病率与对照组存在统计学差异(P<0.05)。结论脂肪肝与GERD显著相关,控制脂肪肝发展可以降低GERD发病率。     

基质细胞衍生因子/趋化因子受体4轴参与胰腺癌细胞侵袭的体外实验研究

Objective To investigate the expression of chemokine receptor (CXCR)4 in human pancreatic cancer cell lines,and its association with proliferation,adhesion and invasion of pancreatic cancer cells.Methods The CXCR4 mRNA and protein in three pancreatic cancer cell lines were detected by RT-PCR and Western blotting,respectively.Confocal microscopy was used to detect the fluorescence intensity induced by SDF-lα in AsPC-1 cells.MTT test was performed to study the proliferation of pancreatic cancer cells.The invasive ability of pancreatic cell lines was determined by transwell invasion assay kit and the adhesive ability was detected by cell adhesive test in vitro.Results There were expressions of CXCR4 mRNA and protein in different extent in three pancreatic cancer cell lines.The strong expression was seen in AsPC-1 cell line,but weak expression in SW1990 cell line.The CXCR4 was functional expressed on AsPC-1 ceils.SDF-1α improved the proliferation,adhesion and invasion of three pancreatic cancer cell lines,especially in AsPC-1 cell line,while the proliferation in SW1990 cell line was weak.But all above effects of the SDF-1α could be inhibited by AMD3100.Conclasions CXCR4 mRNA and protein were expressed in pancreatic cancer cell lines.The efficacy that SDF-1 can increase the invasive ability of pancreatic cancer ceils through SDF-1/CXCR4 axis is closely related to the expression of CXCR4.     

浅析磁疗法治疗颈椎病的临床应用进展

本文简要介绍磁疗法治疗颈椎病的基本原理以及磁疗法治疗颈椎病的临床应用,包括恒磁场和脉冲磁场治疗颈椎病的临床应用以及磁疗法结合其他方法治疗颈椎病的临床应用,对磁疗法在临床应用上存在的问题作了简单讨论。     

医学研究生对博士招生申请审核制改革的态度与意见调查

通过问卷调查法和个人深入访谈法抽样调查了某尚未实施申请审核制改革的医学院校2015级博士研究生、2013级硕士研究生,了解调查对象对医学院校博士招生考试改革——申请审核制的态度和对具体方案的建议。结果显示科学学位学生对申请审核制认可度更高,但学生普遍对该制度存在诸多顾虑,并针对性地为医学院校博士招生实行申请审核制实施方案提供参考性建议。     

基质细胞衍生因子/趋化因子受体4轴参与胰腺癌细胞侵袭的体外实验研究

Objective To investigate the expression of chemokine receptor (CXCR)4 in human pancreatic cancer cell lines,and its association with proliferation,adhesion and invasion of pancreatic cancer cells.Methods The CXCR4 mRNA and protein in three pancreatic cancer cell lines were detected by RT-PCR and Western blotting,respectively.Confocal microscopy was used to detect the fluorescence intensity induced by SDF-lα in AsPC-1 cells.MTT test was performed to study the proliferation of pancreatic cancer cells.The invasive ability of pancreatic cell lines was determined by transwell invasion assay kit and the adhesive ability was detected by cell adhesive test in vitro.Results There were expressions of CXCR4 mRNA and protein in different extent in three pancreatic cancer cell lines.The strong expression was seen in AsPC-1 cell line,but weak expression in SW1990 cell line.The CXCR4 was functional expressed on AsPC-1 ceils.SDF-1α improved the proliferation,adhesion and invasion of three pancreatic cancer cell lines,especially in AsPC-1 cell line,while the proliferation in SW1990 cell line was weak.But all above effects of the SDF-1α could be inhibited by AMD3100.Conclasions CXCR4 mRNA and protein were expressed in pancreatic cancer cell lines.The efficacy that SDF-1 can increase the invasive ability of pancreatic cancer ceils through SDF-1/CXCR4 axis is closely related to the expression of CXCR4.     

高脂血症性胰腺炎

自1952年Klaskin等[1]报告1例原发性高脂血症导致胰腺炎反复发作后,现已明确地认识到高脂血症是胰腺炎的病因之一。鉴于其发生与血清胆固醇升高无关,而与血清三酰甘油(TG)水平显著升高密切相关,故高脂血症性胰腺炎又称为“高三酰甘油血症性胰腺炎”(hypertriglyceridaemia pancreatitis,HLP)。临床上通常以TG>11.30mmol/L(1000mg/dl)或血TG在5.65～11.3mmol/L且出现乳糜样血清即可诊断为HLP。12%～38%的AP患者伴有血脂的异常升高,在某些家族性高脂血症患者和妊娠高脂血症患者中常反复发生AP[2,3]。一、高脂血症的病因根据脂蛋白电泳…