流式细胞仪分析100例正常成人T细胞亚群

<正> 流式细胞仪分析T细胞亚群具有不失真、重复性好和快速等优点,国外已多采用。我们于1986年起,用流式细胞仪分析全血溶解染色的正常国人T细胞及其亚群100例,探讨与国外正常值的差异及其变异范围。     

流式细胞仪检测大鼠外周血T细胞亚群(微量全血法)

本实验利用MRC W/OX系列单克隆抗体和流式细胞仪(Epics C型)首次建立了微量全血检测大鼠外周血T细胞亚群的方法,并报告了正常成鼠外周血T细胞亚群分布(n=60):OX-19+细胞占65.45±5.98％(SD),W3/25~+细胞占47.17±6.45％,OX-8~+细胞占24.26±6.18％。W3/25~+亚群和OX-8~+亚群比值为2.12±0.11(SEM)。部分标本流式细胞仪分析结果和荧光显微镜的结果无明显差异(P>0.05)。在抗体染色条件方面,发现当温育温度过高(30℃)时,染色阳性细胞率有明显下降(P<0.001)。实验证实,用微量全血染色不仅简便、可靠、重复性好,且用血量少。同时避免了常规Ficoll分离法时种种弊病。因此用本法可动态观察大鼠外周血T细胞亚群的变化,为进一步研究在药理、病理和免疫学实验中的细胞免疫功能变化提供了方便。     

用单克隆抗体研究大鼠T细胞亚群的功能

T细胞在机体免疫功能中占有重要地位。用单克隆抗体研究人体和小鼠T细胞亚群的结构和功能国内已有不少论述。但对大鼠T细胞亚群的研究尚无报道。本文就大鼠T细胞的MRC W/OX抗原系统的研究做一个概述。自1977年开始,英国牛津大学William D.病理学院MRC细胞免疫研究所着手研究抗大鼠T细胞亚群的单克隆抗体,迄今已建立了完整的抗大鼠T细胞抗原的MRC W/OX系列。目前该系列单克     

原发性肝癌的免疫化学治疗

应用混合淋巴因子、低剂量5-氟脲嘧啶(5-Fu)治疗67例原发性肝细胞癌(HCC)。结果表明,免疫综合治疗能增强宿主NK、ADCC效应和淋巴细胞转化率。混合淋巴因子附加5-Fu在体外能使癌组织内处于严重抑制状态的NK活性恢复。肝癌切除术后应用免疫化疗宿主免疫功能恢复快,恢复率高,单纯给于免疫综合治疗的病例,其生存期亦明显延长。免疫化疗具有增强机体免疫力,解除免疫抑制和直接抑制肿瘤生长、扩散等多重抗癌功能,在肝癌治疗中,尤其在术后抗复发治疗上有重要价值。     

脾脏切除对大鼠移植性肝癌生长的影响

脾脏是体内最大的淋巴细胞器官,它在肿瘤免疫中占有重要的地位。脾切除对肿瘤生长的影响一直是个有争议的问题。目前研究多集中在脾切除后的非腹腔内肿瘤模型和围绕脾切除和胃癌的关系,但有关脾脏切除对腹腔内与脾脏毗邻脏器肿瘤的实验研究报道极少。因此,我们选择大鼠肝内的移植性肿瘤模型,观察脾切除对肝癌生长的影响。     

脾脏切除对大鼠W256癌肉瘤生长和外周血T细胞亚群变化影响的动态研究

Rats were randomized into splenectomised and sham-splenectomised groups (n = 15 in each). W256 carcinosarcoma cells were implanted in the subcutis of each animal 10 days after splenic operation. T cell subsets of peripheral blood were evaluated serially before and after the operation. The growth rate of implanted tumor and diameter of local tumor in the splenectomised were larger than those of the sham-splenectomised. Simultaneously T cell subsets of peripheral blood presented severe reduction of pan-T and T h/i cells and slight increase of T s/c cells in the splenectomised, all of which still remained at the lower level after tumor inoculation, as compared with the non-tumor and tumor growth in sham-splenectomised (P less than 0.01). From these data, it is evident that splenectomy results in the reduction of T h/i cells and a relative increase of T s/c cells, which shows a close correlation with the rapid growth of the tumor following splenectomy.     

脾脏免疫功能和脾切除后的免疫功能紊乱研究现状

1877年Hewson首先提出脾脏与淋巴系统有关。几年之后Ponfich发现脾脏能去除血液中的颗粒物质。1913年Aschoff指出包括脾在内的网状内皮系统是机体抗感染的重要组成。1919年Morris的实验又进一步支持了脾脏在抗感染中的作用。但直到1952年King报告了5例婴儿脾切除后的暴发性感染(OPSI)病例后,人们才开始逐渐改变对脾脏功能的认识。30多年大量的实验和临床资料都证实:脾脏在感染、肿瘤、移植及寄生虫病等方面的免疫中具有重要的功能。这里我们着重回顾介绍近几年来有关脾脏免疫功能和脾切除后的免疫功能紊乱的研究现状。     

T-CELL SUBSETS OF PERIPHERAL BLOOD AND SPLEEN IN HEPATOCARCINOMA-BEARING RATS

T-cell subsets from peripheral blood and spleen were investigated prospectively by flow cytometry in a murine intrahepatic implanted tumor model. Peripheral blood OX-19 cells (pan T-cells) and W3/25 cells (Th cells) decreased with tumor development in tumor-bearing rats, giving significant differences when compared with healthy rats. No significant changes were found in OX-8 cells (Ts cells). Hence, Th Ts ratio in tumor-bearing rats was lower than that in control group. Meanwhile, on day 17 following tumor implantation, in tumor-bearing rats with intrahepatic or peritoneal metastasis, there were less W3/25 cells and more OX-8 cells than those of hepatoma-bearing rats without metastasis (p<0.01). In the spleen, the phenotypes of lymphocyte markers showed less OX-8 cells and more OX-12 cells (B cells) tumor-bearing rats. It was suggested that, in tumor-bearing host, not only was T-cell immune function inhibited, but also there was abnormal distribution of T-cell subsets. It seems that there is a cl     

脾脏切除对大鼠外周血T细胞亚群变化影响的动态观察

脾脏是直接参与细胞介导免疫调节的一个重要免疫器官,拥有全身循环T细胞的25%和B细胞的10～15%。Ts细胞的发育成熟与脾脏密切相关。切除脾脏势必导致机体细胞免疫功能的紊乱,在这方面,已有不少报道。在对脾切除术后病人的随访观察中发现,无脾患者的外周血T细胞数