9-硝基喜树碱包合物脂质体的制备与评价

目的:制备9-硝基喜树碱包合物脂质体以提高其抗肿瘤效果。方法:饱和水溶液法制备9-硝基喜树碱包合物,考查制备包合物脂质体的最佳方法和工艺,制备9-硝基喜树碱包合物隐形脂质体并评价其药剂学性质及抗肿瘤效果。结果:9-NC包合物脂质体最佳工艺为乙醇注入法以10 mg胆固醇,720 mg磷脂,1.0 g包合物处方制备。所制备的9-NC包合物脂质体平均粒径为(112.10±0.81) nm,包封率为(60.11±1.08)%,且体外抗肿瘤效果显著提高。结论:包合物脂质体是喜树碱类药物的抗肿瘤应用的有效载体。     

Hepatoprotective activity of the ethanol extract of Sarcopyramis Nepalensis

The present study examined the protective effect of the ethanol extract of Sarcopyramis nepalensis (EESN) on agents-induced hepatotoxicity in mice and the possible mechanism. Acute liver injury was induced by administration of either CCl4 or D-GalN. The animals were divided into 5 groups in terms of different treatment: normal group, CCl4 or D-GalN group, silymarin or bifendate group, low dose EESN group (10 mg/kg) and high dose EESN group (30 mg/kg). Liver function was evaluated by detecting the levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The oxidize stress markers were measured, including malondialdehyde (MDA), glutathione peroxidase (GSH) and superoxide dismutase (SOD). Liver tissues were histopathologically examined by hematoxy-lin-eosin (H&E) staining. The acute toxicity study revealed that there was no toxicity of EESN at the dose of 5 g/kg in mice. The levels of ALT and AST in serum, and the MDA level in live tissues were significantly increased and the activities of SOD and GSH substantially decreased in mice after CCl4 or D-GalN treatment. These biochemical and oxidize stress markers were profoundly improved after treatment with EESN at different doses, which was similar to the results of silymarin or bifendate treatment. The histophathological examination revealed the significant improvement in the pathological changes of the liver in EESN-treated mice as compared to those in CCl4 or D-GalN group. It was concluded that EESN possesses potential antioxidant and hepatoprotective properties and has therapeutic potential for liver diseases.     

中药金丹肝泰防治大鼠急性肝损伤实验研究

目的:研究金丹肝泰的护肝作用.方法:用D 氨基半乳糖(D GalN)制备大鼠急性肝损伤模型.通过检测血清丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)活性并观察光镜下肝组织的病理学改变,探讨中药金丹肝泰对急性肝损伤的保护作用.结果:口服金丹肝泰浸膏可显著降低急性肝损伤大鼠血清ALT和AST水平,减轻肝细胞的变性坏死.结论:金丹肝泰对肝细胞具有一定的保护作用.     

2-羟基-5-偶氮苯甲酸钠合成工艺的优化

目的：优化中间体2-羟基-5-偶氮苯甲酸钠的合成工艺，提高该中间体的产量。方法：采用正交设计方法考察苯胺、NaNO2、盐酸、氢氧化钠的用量对2-羟基-5-偶氮苯甲酸钠产量的影响。结果：用量为苯胺、NaNO2各0.115mol，盐酸25mL，氢氧化钠0.245mol时产量最高为24.43。结论：盐酸与苯胺的比例不能低于20mL：0.115mol。苯胺、NaNO2是影响产量的主要因素，盐酸和氢氧化钠对产率均有影响。     

Screening on the Pharmacodynemic Active Parts of Protecting Liver of Peristrope japonica (thunb.)Bremek

Peristropejaponica (thunb .)BremekisaherbofgenusPeristropeofAcanthaceaewhichisusedwithallparts .Itisacridandcool.Ithastheactionsofcleaningfever,detoxication ,XingQiandhemostasia .Thisplanthasbeenusedasausefulfolkmedicineinthetreatmentofsnakebite ,tussis ,…     

金丹肝泰1号对大鼠急性肝损伤防治作用的实验研究

用D-氨基半乳糖（D-GaIN）制备大鼠急性肝损伤模型。方法：检测血清丙氨酸氨基转氨酶（ALT）和血清天门冬氨酸氨基转氨酶（AST）的活性，观察光镜下肝组织的病理学改变，探讨对急性肝损伤的保护作用。结果：口服浸膏可显降低急性肝损伤大鼠血清ALT和AST水平，减轻肝细胞的变性坏死，其作用强度与肝炎宁相当，但弱于联苯双酯。     

九头狮子草保肝护肝有效部位的筛选

目的:初步确定九头狮子草保肝护肝的有效活性部位。方法:用D-氨基半乳糖(D-GlaN)制备大鼠急性肝损伤模型,对总提取物和4个极性部位进行药效学筛选,确定有效部位。结果:大鼠口服九头狮子草醇浸膏和水浸膏均可明显抑制大鼠血清中ALT和AST两种转氨酶的升高(P<0.05),九头狮子草正丁醇部位可抑制D-半乳糖胺所致肝损伤所引起的转氨酶升高。结论:初步确定正丁醇部位为九头狮子草保肝的药效学活性部位。     

虫草洋参胶囊抗疲劳与免疫作用及急性毒性实验

目的:虫草洋参胶囊的抗疲劳、免疫作用、急性毒性试验。方法:以小白鼠游泳时间和常压密闭容器中死亡时间为指标研究抗疲劳及耐缺氧的药理作用;对小鼠碳粒廓清影响的免疫作用;急性毒性试验测定虫草洋参胶囊的最大耐受量。结果:虫草洋参胶囊大、小剂量组游泳时间分别为(5.0±1.3)m in和(4.7±1.5)m in,生理盐水组为(3.7±1.0)m in,差异有极显著性(P(0.01);给药组存活时间(50.7±11.2)m in,生理盐水组为(41.2±15.2)m in,差异有显著性(P(0.05);对小鼠非特异免疫的功能———单核巨噬细胞吞噬功能具有明显促进作用;虫草洋参胶囊在剂量为15g.kg-1.d-1时,对小鼠不引起毒性反应。结论:此复方制剂有抗疲劳和耐缺氧的作用,对免疫有明显的促进作用,且毒性较小。     

金丹肝泰丸治疗急性黄疸型肝炎的疗效观察

金丹肝泰丸是鄂、川一带治疗肝炎的很有效的民间秘方,用于治疗急性黄疸性肝炎、慢性活动性肝炎等.为了进一步研究金丹肝泰丸治疗急性黄疸型肝炎的作用机理,笔者进行了临床观察,并与常规保肝治疗作对照,现报告如下.