应用Dick系统治疗脊柱骨折合并脊髓损伤体会

Dick手术是治疗脊柱骨折合并脊髓损伤的一种新方法。我院自1992年以来应用此技术治疗胸腰椎骨折合并脊髓损伤疾患8例,其中不完全截瘫3例,均恢复正常。完全性截瘫5例,1例双下肢肌力恢复至4级,3例截瘫平面下降2个脊髓节段,1例无明显变化。手术优良率为87.5%。作者认为手术取得良好效果关健在于:力争伤后六小时内进行手术,彻底减压,掌握好螺丝钉进钉位置及     

胸腺修饰诱导同种异体静脉移植的免疫耐受研究

Objective To investigate the possibility of inducing immune tolerance to vein trans-plantation in rats by intrathymic injection of allogene. Methods MHC antigen extracted from splenic cells of donor Wistar rats was intrathymically injected to recipients SD rats, and donor Wistar femur vein was transplanted after 2 weeks. Forty-eight SD rats were randomly divided into 4 groups: group A ( femur vein autograft) ,group B ( femur vein allograft) ,group C ( femur vein allograft with use of immunosuppressant), group D (intrathymus injection). Imaging, histology and immunological assays of these groups were carried out 2 weeks after the transplantation. Results Histologic parameters that were tested were better in group D than those in group B. The concentration of serum IFN-γ in group B was significantly higher than that in groups A, C and D [ ( 86. 707±10.928 ) ng/L vs (29.328±4. 170), (69.076±8.059) and (63.355± 4.895) ng/L respectively, P < 0.05 ]. The concentration of serum IL-4 in group B was obviously lower than in groups C and D [ (23.656±3.369) ng/L vs (29.425±4.174) and ( 31.000±4.659) ng/L re-spectively,P < 0.05 ]. Conclusion Intrathymus injection of allogenic antigen might induce specific im-mune tolerance to femur vein transplantation in rats.     

胸腺修饰诱导同种异体静脉移植的免疫耐受研究

Objective To investigate the possibility of inducing immune tolerance to vein trans-plantation in rats by intrathymic injection of allogene. Methods MHC antigen extracted from splenic cells of donor Wistar rats was intrathymically injected to recipients SD rats, and donor Wistar femur vein was transplanted after 2 weeks. Forty-eight SD rats were randomly divided into 4 groups: group A ( femur vein autograft) ,group B ( femur vein allograft) ,group C ( femur vein allograft with use of immunosuppressant), group D (intrathymus injection). Imaging, histology and immunological assays of these groups were carried out 2 weeks after the transplantation. Results Histologic parameters that were tested were better in group D than those in group B. The concentration of serum IFN-γ in group B was significantly higher than that in groups A, C and D [ ( 86. 707±10.928 ) ng/L vs (29.328±4. 170), (69.076±8.059) and (63.355± 4.895) ng/L respectively, P < 0.05 ]. The concentration of serum IL-4 in group B was obviously lower than in groups C and D [ (23.656±3.369) ng/L vs (29.425±4.174) and ( 31.000±4.659) ng/L re-spectively,P < 0.05 ]. Conclusion Intrathymus injection of allogenic antigen might induce specific im-mune tolerance to femur vein transplantation in rats.     

带血管蒂组织瓣移位修复膝以下软组织缺损和畸形(附5例报告)

本文介绍了临床应用带蒂组织瓣修复膝以下软组织缺损5例,组织瓣均成活,获得满意疗效。同时讨论了所应用组织瓣的优点及手术中的注意事项。     

胸腺修饰诱导同种异体静脉移植的免疫耐受研究

目的 观察大鼠胸腺内注射异基因抗原在同种异体异基因股静脉移植免疫耐受中的作用.方法 将48只SD大鼠随机分为4组:自体股静脉移植组(A组)、异体股静脉移植组(B组)、异体股静脉移植免疫抑制剂组(C组)、胸腺内注射供体组织相容性(MHC)抗原后移植组(D组).于2周后进行影像学、组织学、免疫学检测.结果 组织学检测结果显示:D组、C组急性排斥反应损伤较轻,B组血管壁的各层结构破坏最重,可见大量炎性细胞浸润.B组受体大鼠血清干扰素(IFN)-γ浓度为(86.707±10.928)ng/L,显著高于A、C、D组[(29.328±4.170)、(69.076±8.059)、(63.355±4.895)ng/L,P<0.05];B组受体大鼠血清白细胞介素(IL)-4浓度为(23.656±3.369)ng/L,显著低于C、D组[(29.425±4.174)、(31.000±4.659)ng/L,P<0.05].结论 胸腺内注射异基因MHC抗原可诱导大鼠对同种异体血管移植的特异性免疫耐受.     

胸腺修饰诱导同种异体静脉移植的免疫耐受研究

Objective To investigate the possibility of inducing immune tolerance to vein trans-plantation in rats by intrathymic injection of allogene. Methods MHC antigen extracted from splenic cells of donor Wistar rats was intrathymically injected to recipients SD rats, and donor Wistar femur vein was transplanted after 2 weeks. Forty-eight SD rats were randomly divided into 4 groups: group A ( femur vein autograft) ,group B ( femur vein allograft) ,group C ( femur vein allograft with use of immunosuppressant), group D (intrathymus injection). Imaging, histology and immunological assays of these groups were carried out 2 weeks after the transplantation. Results Histologic parameters that were tested were better in group D than those in group B. The concentration of serum IFN-γ in group B was significantly higher than that in groups A, C and D [ ( 86. 707±10.928 ) ng/L vs (29.328±4. 170), (69.076±8.059) and (63.355± 4.895) ng/L respectively, P < 0.05 ]. The concentration of serum IL-4 in group B was obviously lower than in groups C and D [ (23.656±3.369) ng/L vs (29.425±4.174) and ( 31.000±4.659) ng/L re-spectively,P < 0.05 ]. Conclusion Intrathymus injection of allogenic antigen might induce specific im-mune tolerance to femur vein transplantation in rats.     

胸腺修饰诱导同种异体静脉移植的免疫耐受研究

Objective To investigate the possibility of inducing immune tolerance to vein trans-plantation in rats by intrathymic injection of allogene. Methods MHC antigen extracted from splenic cells of donor Wistar rats was intrathymically injected to recipients SD rats, and donor Wistar femur vein was transplanted after 2 weeks. Forty-eight SD rats were randomly divided into 4 groups: group A ( femur vein autograft) ,group B ( femur vein allograft) ,group C ( femur vein allograft with use of immunosuppressant), group D (intrathymus injection). Imaging, histology and immunological assays of these groups were carried out 2 weeks after the transplantation. Results Histologic parameters that were tested were better in group D than those in group B. The concentration of serum IFN-γ in group B was significantly higher than that in groups A, C and D [ ( 86. 707±10.928 ) ng/L vs (29.328±4. 170), (69.076±8.059) and (63.355± 4.895) ng/L respectively, P < 0.05 ]. The concentration of serum IL-4 in group B was obviously lower than in groups C and D [ (23.656±3.369) ng/L vs (29.425±4.174) and ( 31.000±4.659) ng/L re-spectively,P < 0.05 ]. Conclusion Intrathymus injection of allogenic antigen might induce specific im-mune tolerance to femur vein transplantation in rats.     

小鼠胸腺内注射异基因抗原对异体神经移植免疫反应的影响

目的　探讨胸腺内注射异基因抗原对异体鼠坐骨神经移植存活的影响。　方法　 70只Balb/c小鼠随机分为 5组 :自体神经移植组 (A组 )、异体神经移植组 (B组 )、冷冻后异体神经移植组 (C组 )、应用免疫抑制剂异体神经移植组 (D组 )和胸腺内注射供体组织相容性 (MHC)抗原后移植组 (E组 )。 3周后进行神经电生理检查、组织学检查、混合淋巴细胞培养及迟发性超敏反应的检测。　结果　运动神经传导速度E组为 3 8 2 3 (m /s) ,与D组相比差异无显著性 (P >0 0 5 ) ,且E组优于C组、B组。混合淋巴细胞培养E组为 2 668 3 7(cpm ) ,迟发性超敏反应E组为 0 5 10 (cpm ) ,组织学及电镜检查均证实A组优于E组、E组分别优于C组、D组、B组。　结论　胸腺内注射异基因抗原可诱导对异体坐骨神经移植的特异性免疫耐受。     

经椎弓根内固定治疗胸腰椎骨折脱位12例分析

本文报道了用Ｄｉｃｋ钉和Ｓｔｅｆｆｅｅ钢板螺钉内固定治疗胸腰椎骨折脱位１２例。平均随访７．４个月，无１例发现有神经症状加重或内固定松动等，全部骨折均获得坚固愈合。     

微创椎间盘髓核摘除术

目的：介绍微创腰间盘髓核摘除新技术。方法：我院骨科2004年9月开始,开展微创椎间盘髓核摘除术。至2005年6月底,已开展25人、30节段。结果患者术后第一天即可下地步行功能锻炼。术后5天～7天即可出院,效果良好。结论保护了腰椎的生物力学结构,创伤小,患者恢复快。