关节镜下有限与广泛清理术治疗不同分期 KOA患者的疗效观察

目的：分析关节镜下不同手术方法治疗不同分期的膝关节骨性关节炎（ KOA）患者的临床效果。方法收治的KOA患者157例随机分为A组78例和B组79例,A组采用关节镜下有限清理术、B组采用关节镜下广泛清理术,比较两种手术方法治疗KOA患者的临床效果。结果 A组患者的手术时间、术后恢复时均显著短于B组,差异有统计学意义（ P ＜0？．01）;K-L分级为Ⅰ级、Ⅱ级的患者,术后1个月、6个月及末次随访A、B组患者的Lysholm评分组间比较,差异无统计学意义（ P ＞0．05）;K-L分级为Ⅲ级的患者,B组末次随访的Lysholm评分显著的高于A组,差异有统计学意义（ P ＜0．05）;末次随访时A组患者的膝关节功能优良率（51．28％）与B组（56．96％）比较,差异无统计学意义（ P ＞0．05）;2组术后1、6个月及末次随访VAS评分较术前均显著降低（ P ＜0．05）。结论关节镜下有限清理术与关节镜下广泛清理术治疗KOA患者均具有较好的效果,有限清理术具有手术时间短、恢复快的特点,广泛清理术对K-L分级Ⅲ级的患者效果更好。     

274例盲吸绒毛术后出生小儿远期发育的随访研究

绒毛组织活检,是一项引人注目的产前诊断应用技术。这一技木的应用使胚胎遗传病早期诊断和预防工作进入了一个新阶段。实行绒毛活检虽然才有十余年的历史,但已广泛应用于优生实践,结合细胞遗传学、生化遗传学和分子遗传学技术测定胎儿优劣,决定胎儿取舍,使选优生育在妊娠二个月就可以决断。但是,绒毛取材术对所生小儿有无不良影响,是人们关注而尚处于争议中的问题。因此,我们在86年5月至87年3月对10年来做过绒毛组织取材而保留妊娠的妇女随访到了271例,对其所生的274例小儿进行了系统检查,以了解绒毛组织取材术对胎（小）儿生长、发育及智力的影响。     

首发症状不典型的急性心肌梗死35例误诊分析

急性心肌梗死(AMI)有典型临床表现和典型心电图表现者仅占70%￣80%。一部分AMI患者缺乏典型的临床表现和特征,尤其是首发症状不典型者,早期常延误诊治。本研究对1990￣2003年13年间我院收治的AMI患者118例(其中误诊35例,占29.7%),加以分析,以期提高对AMI的诊断水平。1误诊类型分析1.1以上腹痛伴恶心、呕吐为首发症状常误诊为急性胃炎、急性胃肠炎、急性胆囊炎、急性胃穿孔、急性胰腺炎等。此种情况有8例(6.78%),以中、老年患者居多。AMI的典型临床表现为持续胸骨后剧烈疼痛。当发生下壁心肌梗死时,临床上有2.7%￣17.6%的患者表现为突…     

ISOLATION AND IDENTIFICATION OF cDNA FRAGMENTS AND FULL-LENGTH cDNA DIFFERENTIALLY EXPRESSED IN HUMAN GLIOBLASTOMA CELL LINE BT-325 VERSUS ALL-TRANS RETINOIC ACID INDUCTION

Objective. To investigate the differentiation process of the human glioblastoma cells. Methods. Differential display reverse transcribed-PCR(DDRT-PCR) was used to isolate the genes differentially expressed in control and all-trans retinoic acid treated human glioblastoma cell line BT-325. Routine method of cDNA library screening was performed to clone full-length cDNA. Results. Thirty-six RT-PCR reactions were performed and 64 differentially expressed fragments were recovered, amplified and cloned. Of them,46 ESTs were sequenced and delivered into the GenBank. The homology comparison us-ing BLAST algorithm revealed that 22ESFs are highly homologous with the known genes and many of them play impor-tant roles in the cell differentiation progress. A dot-blot hybridization was conducted to certify the differemiation expres-sion. The result showed that 27 EST clones are expressed at different level in control and all-traus retinoic acid treated BT-325 cells. A full-length cDNA was cloned using the EST-HGBB098.Conclusion. DDRT-PCR was a simple and effective method to segally analyze the differentially expressed genes.