通冠胶囊对大鼠颈动脉球囊损伤术后血管内膜VEGF表达的影响

目的 探讨通冠胶囊对血管损伤后内膜增生及VEGF表达的影响.方法 制备大鼠左颈总动脉球囊损伤模型,将54只SD大鼠随机分为通冠胶囊组、盐水对照组和辛伐他汀对照组,于术后第4周、第8周和第12周处死动物.分别进行取材检测指标:HE染色及弹力纤维染色后光镜下观察管壁增生情况,计算机图像分析内、中膜面积及其比值观察通冠胶囊对新生内膜形成的影响.血管内皮生长因子(VEGF)免疫组织化学法检测明确损伤后血管内皮修复情况.结果 通冠胶囊组在4周、8周和12周时免疫组化VEGF表达较盐水对照组明显升高(均P<0.01),辛伐他汀组的变化趋势与通冠胶囊组相似.通冠胶囊组4周、8周与同时段辛伐他汀组比较无统计学差异(均P>0.05),12周较辛伐他汀组明显升高(P<0.05).球囊损伤后4周各组内膜增生明显,8周时形成的增生内膜减少,12周时形成的增生内膜明显减少.通冠胶囊组在损伤后4周、8周、12周形成的增生内膜较盐水对照组显著减少(均P<0.01),内膜面积与中膜面积的比值较盐水对照组明显降低(均P<0.01).结论 通冠胶囊可以促进大鼠颈总动脉球囊损伤处的VEGF的表达,并减少新生内膜的形成.     

电针对局灶性脑缺血大鼠缺血灶周围区胶质纤维酸性蛋白表达的影响

目的:观察电针对不同时间段局灶性脑缺血大鼠缺血灶周围区胶质纤维酸性蛋白(GFAP)表达及星形胶质细胞超微结构的影响,探讨电针治疗脑缺血疾病的可能作用机制。方法:随机将90只W istar大鼠均分为假手术组、模型组和电针组,每组又分为1h、1天、3天、7天和21天5个时间段小组,每小组6只大鼠,采用热凝闭大脑中动脉建立局灶性脑缺血模型,电针组取"百会"、"大椎"穴,选用疏密波,5~10次/s,电压2~3V,留针30m in。每天1次,分别治疗1h、1天、3天、7天和21天后取材。采用透射电镜观察不同时段各组大鼠脑缺血灶周围区星形胶质细胞的超微结构,采用免疫组化法检测星形胶质细胞GFAP表达的变化。结果:电镜下可见星形胶质细胞在缺血性脑损伤后肿胀、增多,电针组的星形胶质细胞肿胀程度较模型组减轻。模型组和电针组GFAP表达在1h时与假手术组比较,差异无显著性意义(P>0.05),在1天、3天、7天及21天时,模型组和电针组GFAP表达都明显高于假手术组(均P<0.01),尤其是在7天时表达最强,而电针组GFAP表达在各时段均明显低于模型组(P<0.05,P<0.01)。结论:脑缺血后星形胶质细胞出现反应性增生活化,电针可减轻星形胶质细胞肿胀,抑制星形胶质细胞GFAP的过度表达,电针治疗脑缺血的机制可能与其干预星形胶质细胞的活化状态有关。     

益气活血汤治疗不稳定型心绞痛的机制探讨

目的 初步探讨益气活血汤治疗不稳定型心绞痛的作用机制.方法 将41例患者随机分为两组.对照组20例给予冠心病常规治疗;治疗组21例在对照组治疗基础上加益气活血汤治疗.观察治疗前后血清一氧化氮(NO)、内皮素(ET)、血浆纤维蛋白原(FG)、血管性假性血友病因子(vWF)、血浆组织型纤溶酶原激活剂(t-PA)、纤溶酶原激活剂抑制物(PAI-I)的变化.结果 治疗组临床疗效优于对照组(P<0.05).在降低NO,ET,FG,vWF,PAI-I,提高t-PA方面较对照组明显(P<0.05～0.01).结论 益气活血汤可有效防治不稳定型心绞痛;其机制可能与其对血栓形成前状态(PTS)凝血和纤溶系统部分敏感分子标志物的影响等有关.     

内皮祖细胞在冠心病治疗中的中西医研究新进展

内皮祖细胞(EPCs)作为血管内皮细胞的前体细胞不仅参与人胚胎血管生成,同时也参与出生后血管新生和内皮损伤后的修复过程。在冠心病的发生、发展中也扮演了重要角色。通过动员或移植EPCs促进内皮修复、血管新生,具有重要的临床意义。中医药治疗冠心病有着悠久的历史,具有与西医不同的特殊性,通过整体观念指导下的综合作用以及中药的特殊归经理论,能够同时作用于疾病发生的不同环节,多途径、多层次、多环节、多靶点直接或间接地影响冠心病的发生与发展,临床和实验研究已发现一批有效的中药可能具有调节EPCs特性功能,如作用于动员、分化、归巢、黏附和整合至血管等环节,有望最终改善心血管病患者的临床预后。     

加服益气活血方对冠心病介入术后患者bFGF的影响

目的:观察加服益气活血方对冠心病介入术后患者bFGF的影响。方法:将60例冠心病PCI术后患者随机分为2组,对照组30例予常规西药治疗(阿司匹林,氯吡格雷),治疗组30例在对照组治疗基础上加服益气活血方,两组疗程均为1个月。结果:治疗1个月后分别检测两组bFGF水平,治疗组为6.99±2.6pg/ml,对照组为5.69±1.81pg/ml,两组均较同组治疗前改善,以治疗组改善程度更明显(P<0.05)。结论:加用益气活血方能促进冠心病PCI术后患者的bFGF表达。     

参附注射液治疗心力衰竭研究概述

参附注射液是由古验方“参附汤”改制剂型而成,参附汤出自《世医得效方》,为一首大温大补、急救暴脱的方剂。由人参、附子两药配伍组成,能振奋心肾之阳气,蠲阴寒之水气。《医宗金鉴·删补名医方论》所说:“两药相须,用之得当,则能瞬间化气于乌有之乡,顷刻升阳于命门之内,方之最神捷者也。”现代药理研究也证明参附注射液具有多种药理作用,目前该药已广泛应用于临床。本文对近10年来(1994-2004)参附注射液在心力衰竭中的临床应用及其药理机制的研究作一综述。1参附注射液治疗充血性心力衰竭1.1对症状的改善李慧[1]将67例充血性心力衰竭患者随…     

浴足疗法协同治疗原发性高血压病的疗效观察

目的:观察自拟浴足方浴足协同治疗高血压病的临床疗效。方法:将符合纳入标准的原发性高血压病患者120例随机分为治疗组及对照组各60例,治疗组内服基础降压药,加用自拟浴足方浴足;对照组内服基础降压药,加用温水浴足。疗程为8周,观察并比较两组患者治疗前后血压及临床症状等指标的变化。结果:两组患者治疗后收缩压及舒张压均明显下降(P0.05),治疗组优于对照组(P0.05);在改善临床症状方面,治疗组总有效率为100%,对照组为86.67%,两组比较有显著性差异(P0.05)。结论:自拟浴足方联合基础降压药具有协同降压作用,降压疗效优于单纯基础降压药物治疗,且能改善临床症状,简便易行,可在临床推广应用。     

通冠胶囊对大鼠颈动脉内皮损伤后血管VEGF eNOS表达和血清NO浓度的影响

目的:探讨通冠胶囊促进动脉损伤内皮修复的分子机制。方法:建立大鼠左颈总动脉内皮损伤模型,24只雄性SD大鼠随机等分为治疗组、对照组和模型组,治疗组给予通冠胶囊水煮液(600mg/kg)灌胃,对照组给予辛伐他汀(2mg/kg),模型组予生理盐水(10mL/kg);8周后,取材HE染色和弹力纤维染色,计算机图像分析系统检测颈动脉内皮损伤处内膜面积(intima area,IA)、中膜面积(media area,MA)和内膜/中膜面积比(IA/MA),免疫组织化学法检测VEGF和eNOS的表达,分光光度法测定血清NO水平。结果:治疗组、对照组的IA和IA/MA均小于模型组(P<0.01),治疗组、对照组血管内膜VEGF、eNOS表达和血清NO水平与均高于模型组(P<0.05或P<0.01),治疗组的血清NO水平高于对照组(P<0.05)。结论:通冠胶囊促进损伤内皮修复的分子机制可能与VEGF-AKT-eNOS-NO信号转导通路有关。     

益气活血汤对大鼠血管内皮损伤后VEGF的影响

目的:探讨益气活血汤对大鼠血管内皮损伤后VEGF表达的影响。方法:健康雄性SD大鼠90只,体重200²20 g,随机分为中药组(n=30)、西药组(n=30)和对照组(n=30),三组大鼠高脂饲料喂养4周后行颈动脉内皮球囊损伤的造模。术后普通饲料喂养,中药组予益气活血汤灌胃8周,西药组予阿托伐他汀钙片灌胃8周,对照组予生理盐水灌胃8周。三组大鼠灌胃8周后处死,用免疫组化法观察对损伤血管内皮的影响。结果:对照组血管内膜不规则增厚,中药组、西药组内膜增生程度较对照组轻(P<0.05)。结论:益气活血汤能使大鼠颈动脉内皮损伤后内膜增生程度减低,可能与其促进VEGF的表达、抑制炎症反应和改善血管内皮功能有关。     

电针对局灶性脑缺血大鼠缺血灶周围区星形胶质细胞的影响

BACKGROUND： Astrocytes react sensitively to cerebral ischemia, causing reactive proliferation and activation, which may contribute to their effect in protecting or injuring neuronal regeneration. Whether acupuncture, as a treatment for cerebral ischemia, regulates the activated state of astrocytes has become a focus of recent investigations. OBJECTIVE： To observe the effects of electroacupuncture （EA） on ultrastructure changes and reactive proliferation of astrocytes in the marginal zone of focal cerebral ischemia in rats. DESIGN, TIME AND SETTING： Randomized, controlled animal study. This study was performed at the Experimental Animal Center of Guangzhou University of Traditional Chinese Medicine between December 2007 and July 2008. MATERIALS： A total of 90 male Wistar rats were randomly divided into sham operated, model and EA groups. Each group was subdivided into 1 hour, 1, 3, 7, and 21 days post-cerebral ischemia groups, with six animals for each time point. Rabbit anti-rat glial fibrillary acidic protein （GFAP） and goat anti-rabbit IgG/tetramethylrhodamine isothiocyanate were provided by Beijing Biosynthesis Biotechnology. The G-6805 electric acupuncture apparatus was provided by Shanghai Huayi. METHODS： Heat-coagulation-induced occlusion of the middle cerebral artery was performed to establish a model of focal cerebral ischemia, in the model and EA groups. Middle cerebral arteries were exposed without occlusion in sham operated group. EA was applied immediately after surgery in the EA group, 4/20 Hz, 2.0-3.0 V, 1-3 mA, to Baihui（GV 20） and Dazhui（GV 14）, for 30 minutes. The treatment was performed once a day. The sham operated and model groups did not receive acupuncture. MAIN OUTCOME MEASURES： In the marginal zone of focal cerebral ischemia in rats at different time points after intervention, the ultrastructure changes of astrocytes were observed by using transmission electronic microscopy. GFAP expression in astrocytes was also measured by laser confocal scanning microscopy. RESULTS： Cell swelling and rapid proliferation of astrocytes were observed following cerebral ischemia. In comparison to the model group, the degree of swelling of astrocytes was significantly decreased in the EA group. Compared with the sham operated group at hour 1 post-surgery, there was no significant difference in the expression of average fluorescence intensity of GFAP between the EA and model groups （P 〉 0.05）, while the expression of GFAP in both the EA and model groups increased significantly at days 1, 3, 7 and 21 post-surgery （P 〈 0.01）. The expression of GFAP in EA group was also significantly lower than in the model group （P 〈 0.01, P 〈 0.05）. CONCLUSION： Ultrastructural changes and reactive proliferation of astrocytes appear in the marginal zone of focal cerebral ischemia in rats. EA can relieve the degree of swelling of astrocytes and inhibit GFAP overexpression by activated astrocytes. These effects may be related to its ability to regulate the activated state of astrocytes.