基于代谢组学技术研究艾灸“足三里”对慢性萎缩性胃炎大鼠胃组织代谢物的影响

目的:采用代谢组学的方法观察正常及疾病状态下,艾灸"足三里"对胃组织代谢产物的影响,从代谢组学角度探讨阳明经穴位与胃之间相对特异性联系的基础。方法:将56只SD大鼠分为正常组、正常+艾灸组、模型组、模型+艾灸组,每组14只。通过N-甲基-N′-硝基-N-亚硝基胍联合乙醇灌胃、饥饱失常法复制慢性萎缩性胃炎(CAG)大鼠模型。正常组和模型组不予以治疗,正常组+艾灸组和模型组+艾灸组予以艾灸"足三里""中脘"穴治疗,每次15min,每日1次,共2周。HE染色观察大鼠胃组织病理变化,核磁共振氢谱(1 H NMR)技术检测胃组织代谢物并用Chenomx NMR Suite软件分析所得数据。结果:模型组大鼠胃组织病理改变符合CAG病理诊断标准,模型+艾灸组大鼠胃组织病理改变较模型组有一定程度的改善。与正常组比较,模型组大鼠胃组织中的乳酸、谷胱甘肽、N-乙酰天门冬氨酸、尿苷二磷酸葡萄糖、肌糖升高(P0.05),亮氨酸、甜菜碱、磷酸胆碱、尿嘧啶降低(P0.05);艾灸"足三里"后浓度发生逆转的代谢物有谷胱甘肽、N-乙酰天门冬氨酸、磷酸胆碱、尿嘧啶(和模型组比较,P0.05)。与正常组比较,正常+艾灸组胃组织中的肌醇升高(P0.05),而3-羟基丁酸、丝氨酸、尿苷二磷酸葡萄糖降低(P0.05)。结论:从代谢组学角度证明了胃经穴位与胃之间存在一定的相对特异性联系,谷胱甘肽、N-乙酰天门冬氨酸、磷酸胆碱、尿嘧啶等差异性代谢物可能是介导艾灸胃经穴位产生效应的代谢物质基础。     

三通四联法治疗膝关节骨性关节炎42例

膝关节骨性关节炎是一种慢性关节疾病,又称增生性关节炎、肥大性关节炎、老年性关节炎等.本病好发于50岁以上中老年人,女性多见,临床上主要表现为膝关节疼痛、功能障碍及膝关节畸形,影响患者的生活质量.目前尚无有效治疗该病的药物和方法.笔者采用三通四联法治疗本病42例58只膝,疗效满意,现报告如下.     

基于代谢组学的针灸作用机制研究进展

代谢组学是新近兴起的一种系统生物学研究手段,具有整体性、动态性反应生物体接受刺激时所作出的代谢物层面的变化,与中医整体观十分吻合,因此广泛用于中医中药学的研究中,在针灸学机制的研究中早已将代谢组学作为一种有效的研究手段,近年来针灸代谢组学的研究日趋增多,在揭示针灸作用机制方面也取得了一定的成果,故对现今的研究做一综述,以愿能为代谢组学广泛应用于针灸的现代化研究提供新思路。     

针灸治疗子宫内膜异位症20例

子宫内膜异位症是指具有生长功能的子宫内膜,生长在宫腔壁表面以外的异常位置而引起的病变,是妇科难治病、多发病。疼痛是该病的主要症状,主要表现为继发性、进行性的痛经。笔者自2009年12月至2011年6月运用针灸治疗以痛经为主症的子宫内膜异     

刺血配合龙胆泻肝汤治疗带状疱疹验案

带状疱疹是由水痘-带状疱疹病毒引起的急性疱疹性皮肤病。该病毒潜伏于脊髓后根神经节的神经元中，当细胞免疫功能下降时被激活而发病。本病的发病特点是突然发生簇集性水疱，沿一侧周围神经呈带状分布，伴有神经痛，多发于春秋季节，成人多见，病程2-4周。发疹前常有发热、倦怠、食欲减退、患部皮肤烧灼感或神经痛等前驱症状，     

Investigating the effects of moxibustion on serum metabolism in healthy human body based on the <Superscript>1</Superscript>H NMR metabolomics technology

Objective

To investigate the effects of moxibustion on the serum metabolism in healthy human body based on the 1H nuclear magnetic resonance (¹H NMR) metabolomics technology, and to find the differences in metabolites, as well as to elucidate the effects of moxibustion on healthy human body from the viewpoint of global metabolism.

Methods

Sixty subjects of healthy young men from the enrolled students were randomly divided into a moxibustion group and a control group using random number table, with 30 cases in each group. Subjects in the moxibustion group accepted mild moxibustion on the right Zusanli (ST 36), once a day, 15 min for each time, and continuous treatment for 10 d; those in the control group did not receive any intervention. There were 28 cases in the moxibustion group and 23 cases in the control group after interventions. On the 1st day, 5th day and 10th day of the intervention, serum samples were collected from subjects of the two groups, and metabolic spectra were obtained by the ¹H NMR technology.

Results

Before and after the intervention, serum ¹H NMR of the moxibustion group was significantly different, while the difference was insignificant in the control group. Metabolite changes in the moxibustion group were mainly in low density lipoprotein (LDL)/very low density lipoprotein (VLDL), valine, isoleucine, leucine, lactic acid, glutamine, citric acid, polyunsaturated fatty acids, creatine, glycine, glycerol, glucose, tyrosine, histidine, formic acid, alanine, lysine, acetic acid, and glutamic acid.

Conclusion

Moxibustion can cause changes of serum metabolic patterns in healthy human by influencing the concentrations of branched-chain amino acids, polyunsaturated fatty acids, and other metabolites to strengthen body's metabolisms of amino acids and fatty acid.

     

基于1H-NMR技术探讨艾灸与针刺对血清代谢物的调节作用

目的：基于核磁共振氢谱代谢组学技术（¹H-NMR）探讨艾灸与针刺对健康青年志愿者血清代谢物调节作用的差异性,从代谢物层面揭示两者的作用特点及差异。方法：将60例健康青年男性志愿者随机分为艾灸组与针刺组,每组各30例,艾灸组采用温和灸作用于右侧足三里穴,每天1次,每次15min,连续干预10天;针刺组采用不锈钢针针刺右侧足三里穴,每天1次,每次15min,连续干预10天。两组在干预0、5、10天清晨采集受试者血清,利用¹H-NMR技术对血清代谢物进行检测。结果：干预0、5天时艾灸组与针刺组的代谢模式无显著性差异;干预10天时艾灸组与针刺组的代谢模式可显著区分;艾灸与针刺干预5天后血清中表现出差异的代谢物有Creatine和Glycine（P<0.05;P<0.01）;干预10天后血清中表现出差异的代谢物有LDL/VLDL、Valine、Isoleucine、Leucine、Lactate、Glutamine、Creatine、Glucose（P<0.01）和Glycine（P<0.05）;随着干预时间的变化,艾灸与针刺对血清代谢物的调节作用有差异。结论：艾灸与针刺右侧足三里对血清代谢物有明显的调节作用,随着干预时间的变化,两者的调节作用发生变化。     

Clinical observation on wheat grain-sized cone moxibustion combined with fire needle for dorsal wrist ganglion

Objective

To observe the clinical effect of wheat grain-sized cone moxibustion combined with fire needle therapy in the treatment of dorsal wrist ganglion (DWG).

Methods

A total of 63 patients with DWG were randomized into a treatment group (n=32) and a control group (n=31). Cases in the control group were treated with fire needle therapy. Based on the therapy given to the control group, cases in the treatment group were additionally given wheat grain-sized cone moxibustion therapy. Then the therapeutic efficacies and recurrence rate were evaluated.

Results

After treatment, the overall effective rate was 93.8% in the treatment group, versus 77.4% in the control group, and the between-group comparison showed a statistical significance (P<0.05); 6 months after the treatment, the recurrence rate in the treatment group was obviously lower than that in the control group (P<0.05).

Conclusion

The long-term and short-term effects of wheat grain-sized cone moxibustion combined with fire needle therapy in the treatment of DWG are better than those of fire needle therapy alone, and therefore, worth popularizing.

     

鼠神经生长因子结合“三通四联”针灸综合疗法治疗顽固性周围性面瘫临床研究

目的:观察鼠神经生长因子结合"三通四联"针灸综合疗法治疗顽固性周围性面瘫的临床疗效。方法:选取60例顽固性周围性面瘫患者,按随机数字表法分为对照组和观察组各30例。对照组采用"三通四联"针灸综合疗法治疗,观察组在对照组基础上加鼠神经生长因子穴位注射。观察2组治疗前后中医证候积分,比较临床疗效。结果:观察组临床总有效率为93.3%,高于对照组70.0%,差异有统计学意义(P0.05)。治疗前,2组中医证候积分比较,差异无统计学意义(P0.05)。治疗后,2组中医证候积分较治疗前降低(P0.05),且观察组中医证候积分低于对照组(P0.05)。结论:鼠神经生长因子结合"三通四联"针灸综合疗法治疗顽固性周围性面瘫可以改善患者临床症状,临床疗效显著。     

针灸治疗顽固性失眠23例

顽固性失眠,属于中医学不寐的范畴[1],临床主要以经常不能获得正常睡眠为特征,轻者入睡困难或睡而易醒,醒后不能再睡,也可以表现为时睡时醒,严重者则整夜不能入睡,属于现代医学社经衰弱范畴,已经成为严重困扰患者的常见疾病之一,使患者的生活质量急剧下降.