12例重型肝炎患者的护理

目的运用心理护理、专科护理、并发症的护理等措施,控制重型肝炎的发生发展。方法采取心理护理、饮食护理、生活护理、并发症护理。结果运用各种有效的护理措施,有效地减少患者并发症的发生,提高生存率。结论加强护理,可以大大降低重型肝炎患者死亡率,从而提高其存活率。     

细胞荧光素酶报告基因试验受CpG岛甲基化状态影响

Objective To investigate the effects of methylation status of CpG islands of endogenous E-cadherin (CDH1) gene on the promoter activity of corresponding genes in reporter assays. Methods The methylation statuses of CpG island of CDHI in 8 different cell lines were detected by methylation-specific PCR. CDH1 protein was analyzed by Western blotting. Two sets of pGL3 reporter vectors with different genotypes/haplotypes of the CDH1 promoter were constructed [pGI3-A(-73)/-C(-73)pGL3-H1/-H4]and used to transfect these cell lines. The differences between these promoter reporter vectors were analyzed by t-test. Results (1) CDH1 CpG island was unmethylated in AGS, MCF7, MKN74, and PC-3 cell lines,expressed in MCF7, MKN74, and PC-3 ,but not in AGS. Expression of CDH1 was silenced by methylation in HeLa, BGC823, A549, and RKO cell lines. (2) In the four CDH1 -unmethylated MCFT, M KN74, PC-3, and AGS cell lines ,the promoter activities of pGI3-C(-73)(as 0. 78±0. 10,0. 17±0.01,0. 11±0. 01,1.19±0. 18)were significantly higher than those of pGL3-A(-73)(as 0. 30±0. 08,0. 07±0. 01,0. 07±0. 01,0. 39±0. 04) (t values are -6. 298, -12. 349, -8. 128, -7.388, and P<0. 01). However, in the four C DH1 -methylated HeLa, BGC823, A549, and RKO cell lines, the promoter activity of pGL3-C(-73)(as 0. 09±0. 02,0. 13±0. 02,0. 05±0. 01,0. 01±0. 00) was significantly lower than that of pGL3-A(-73)(as 0. 16±0. 01,0.25±0.01,0. 11±0.03,0.03±0.00) (t valued at 5.958,11. 189,3. 661,13. 866,and P<0.05). (3) In the unmethylated MKN74 and methylated RKO cell lines, the promoter activities of pGI3-H1/-H4 were obviously and contrarily different(as 1.57±0. 23/0. 94±0. 06 and 0. 38±0. 02/0. 50±0. 04 ,t values were 4. 577 and -4. 915 ,P values were 0. 010 and 0. 003). Conclusion The methylation status of CpG island of the target gene in the tested cell lines affects the promoter activity in Reporter Assay significantly. The most active one may be the most suppressive one.     

老年白内障术后并发症的预防及护理

老年白内障术后并发症的预防及护理中国人民解放军总医院张宝珍白内障是眼科常见病、多发病，是致盲的主要原因之一，常见的白内障有老年性、外伤性、并发性和先天性４种［１］。已被列为国家３项康复之首。我院于１９８０～１９９２年共做老年白内障手术１４６８例，通过...     

改良孕中、晚期羊水细胞培养制备染色体方法的探讨

目的改良孕中晚期羊水细胞培养制备染色体技术。方法收集45例孕24-32w具有产前诊断指征的的孕妇羊水,根据羊水细胞生长特点,在培养过程中调整换液时间及培养方式收获细胞。结果45例孕中晚期羊水细胞培养全部成功。结论该方法细胞岛多,染色体有效分裂相多,培养成功率高,可满足临床产前诊断的要求。     

利用Excel进行医学统计方差分析

在科学研究中,经常要对收集到的数据进行各种医学统计分析,而分析数据时,大多使用较著名的统计分析软件包,如ＳＡＳ、ＳＰＳＳ等。这些统计分析软件包功能强大,而且可作各种复杂的多变量分析。然而,这些软件包过于庞大,且软件费用过大,一般单位无法购买,而在医学...     

肠粘膜脱垂声像图表现

<正> 近十几年来,B型超声已应用于肠道病变的诊断,由于肠内气体严重防碍了对肠内进行超声检查,所以过去一段时间对肠内超声寥寥无几,应用生理盐水灌肠可以使肠管充盈扩张,并排除肠管内气体,从而形成良好声学界面,获得肠内各部位炎症和粘膜脱垂声像图。 140例经纤维结肠镜证实结肠炎病人中有21例合并肠粘膜脱垂占15％。我们做了生理盐水灌肠超声检查。此种方法能显示从直肠到回盲部的全部结肠轮廓。主要的声像图表现为肠壁增厚和脱垂的肠粘膜向     

150例正常直肠声像图表现

本文对经结肠镜检证实的正常直肠患者，进行超声观察水灌肠后的直肠形态部结构声像图特征，报告如下。1一般资料本组互50例，男88例，女62例，年龄20～65岁，采用岛津SDL-300实时超声显像仪，探头频率3．5MHz，检查当日禁食，口服20％甘露醇250ml加水500ml，肠道清洁后，用生理盐水100～150ml灌入直肠及部分乙状结肠，由肛门口直接探查可获得正常直肠结构声像图。2正常直肠声像图2．1直肠灌水后，肠管充盈，呈弯曲扩张，衬托出清晰前边缘和粗细不等的肠腔，在盆隔以上的直肠盆部（壶腹部），肠壁呈三层结构，自内向外分别为粘膜层，肌层和…     

医学科研SAS 8.2软件与Excel电子表格的应用

人类已进入信息时代，要在大量的信息中获得有科学价值的结果，必须对信息进行存储、整理、统计和绘制图表。SAS8．2统计软件包和EXCELXP电子表格软件这两种软件结合在一起，使医学科研工作者更进一步提高统计分析的速度，确保统计分析的质量，来实现繁冗的数据处理工作。