谈耳鼻喉科教学中多媒体技术的应用

多媒体教学技术应用于耳鼻喉科教学,具有直观性好、动态感强、信息量大等优点,能使教学信息直接、形象、具体、生动地展现给学生,让学生体会到学习的乐趣,是耳鼻喉科现代化教学的理想模式.     

乙醇对人绒毛孕酮分泌的影响

本工作利用灌流技术观察了四种不同浓度的乙醇(0.5％、1％、2.5％、5％)对妊娠早期人工流产新鲜胎盘绒毛分泌孕酮的影响。结果表明,乙醇具有促进孕酮分泌的作用,并存在剂量依赖的关系。提示乙醇可能破坏胎盘激素内分泌的平衡,从而影响胎儿的正常生长与发育。     

Deletion of decay-accelerating factor (CD55) exacerbates autoimmune disease development in MRL/lpr mice

Decay-accelerating factor (DAF, CD55) is a glycosylphosphatidylinositol-anchored membrane protein that restricts complement activation on autologous cells. It is also a ligand for CD97, an activation-associated lymphocyte antigen with seven transmembrane domains. It is widely expressed on cells of both the hematopoietic and nonhematopoietic lineages. Although deficiency of DAF on human erythrocytes is associated with the hemolytic anemia syndrome paroxysmal nocturnal hemoglobinuria, the in vivo biology of DAF is still poorly understood. We addressed the in vivo function of DAF in a knockout mouse model and describe here that deletion of DAF exacerbates autoimmune disease development in MRL/lpr mice, a model for human systemic lupus erythematosus. Compared to DAF-sufficient littermate controls, DAF-deficient female MRL/lpr mice developed exacerbated lymphadenopathy and splenomegaly, higher serum anti-chromatin autoantibody levels, and aggravated dermatitis. Consistent with the phenotype of aggravated dermatitis in DAF-deficient mice, Northern and Western blots and immunofluorescence studies showed DAF to be expressed abundantly in the mouse skin, suggesting that it may play a particularly important role in this tissue. Histology and immunostaining demonstrated inflammatory infiltrate and focal C3 deposition in early skin lesions, mostly along the dermal-epidermal junction. These results reveal a protective function of DAF in the development of a systemic autoimmune syndrome and suggest that dysfunction or down-regulation of DAF may contribute to autoimmune disease pathogenesis and manifestation.     

The prevalence of stroke and related risk factors among residents aged ≥ 40 years in Chongqing,Southwest China

Journal of Public Health - China bears the largest global stroke burden, yet little is known about its rates in Chongqing, southwest China. We aimed to investigate the prevalence and related risk...     

IL—12提高DNA疫苗诱导小鼠抗HBV皮下移植瘤的免疫研究

目的 观察ＩＬ－２真核表达载体（ｐＷＲＧ３１６９）对ＨＢＶ－ＳＤＮＡ疫苗（ｐＣＲ３．１－Ｓ）诱导Ｂａｌｂ／ｃ小鼠（Ｈ－２＾ｄ）的特异性细胞免疫应答及其对稳定表达ＨＢｓＡｇ的小鼠肥大细胞瘤Ｐ８１５细胞（Ｐ８１５－ＨＢＶ－Ｓ）成瘤性的影响。方法肌肉注射ＤＮＡ疫苗,背部皮下接种Ｐ８１５－ＨＢＶ－Ｓ细胞,观察成瘤情况,４ｈ＾５１Ｃｒ释放法检测小鼠脾细胞ＣＴＬ活性。结果 对照组、ｐＣＲ３．１－Ｓ及共同免疫     

阻塞性睡眠呼吸暂停患者血清甲状腺功能指标变化及其影响因素

目的:探讨阻塞性睡眠呼吸暂停患者,血清甲状腺功能指标变化情况,以及相关影响因素。方法:选取2017年6月至2020年6月江门市第二人民医院收治的阻塞性睡眠呼吸暂停患者60例作为研究对象,根据患者的病情,将其分为轻度组(n=20)、中度组(n=21)和重度组(n=19),同时选取非阻塞性睡眠呼吸暂停患者25例作为对照组,了解每组患者的血清甲状腺功能指标情况。结果:中度、重度非阻塞性睡眠呼吸暂停患者的BMI、TSH等指标,明显高于轻度组与对照组。结论:阻塞性睡眠呼吸暂停患者的间歇性缺氧可导致甲状腺激素水平下降。     

中医正骨手法治疗肱骨髁上骨折的临床疗效分析

目的探讨中医正骨手法治疗肱骨髁上骨折的临床疗效。方法选取本院2017年1月至2019年2月收治的80例肱骨髁上骨折患者,采用随机数表法分为两组,每组40例。对照组行常规切开复位内固定术治疗,观察组行中医正骨手法治疗,比较两组临床疗效、肘关节功能评分和并发症发生率。结果治疗后,与对照组比较,观察组总有效率较高,治疗4、8、12周肘关节功能评分高,并发症发生率低,差异有统计学意义(P<0.05)。结论肱骨髁上骨折患者实施中医正骨手法治疗可提高临床疗效,改善肘关节功能,降低并发症发生率。     

miR-1253, a novel tumor suppressor gene in colon cancer,is associated with poor patients prognosis

Clinical and Experimental Medicine - MiR-1253 has been reported to play vital roles in a variety of cancers. However, its function in the progression and prognosis of colon cancer remains unclear....     

miR-548d抑制骨肉瘤细胞的增殖和迁移

目的:探讨miR-548d对骨肉瘤细胞增殖和迁移的影响。方法:通过Real time PCR及Western blot检测miR-548d和KRAS在30例骨肉瘤组织以及293、MG63和U2OS细胞中的表达情况。对30例骨肉瘤组织中miR-548d和KRAS含量的相关性进行分析，随后通过报告基因实验印证miR-548d对KRAS的靶向作用。MG63细胞中分别过表达或沉默miR-548d后，通过Western blot实验分析KRAS的变化情况，MTT实验观察miR-548d对细胞增殖的影响，Transwell实验观察miR-548d对其迁移能力的影响。结果:骨肉瘤组织及细胞中miR-548d表达较低，KRAS表达较高。在骨肉瘤组织中miR-548d与KRAS的表达呈负相关。报告基因实验证明miR-548d可以直接打靶KRAS。Western blot指出miR-548d可以抑制KRAS的表达。最后MTT和Transwell实验指出miR-548d可以抑制MG63细胞的增殖与迁移。结论:miR-548d可以通过打靶KRAS抑制骨肉瘤细胞的增殖和迁移。     

会阴部上皮样血管肉瘤临床病理分析

目的:探讨会阴部软组织上皮样血管肉瘤的临床病理特点、诊断、鉴别和免疫表达。方法:分析5例会阴部软组织EAS临床资料、光镜和免疫表达情况,并复习相关文献进行讨论。结果:光镜下肿瘤成分几乎全部为上皮样肿瘤细胞组成,细胞排列成小巢状、围绕血管成岛状、片状或条索状,可见原始血管结构形成,免疫组化:瘤细胞CD34、CKpan、EMA、Vimtin、CD31及CD99(+),KI-67(10%～25%),而SMA、S-100、Myoglohin、MLA及HMB45(-)。结论:发生于会阴部皮下软组织的EAS罕见,准确诊断较困难,在仔细观察HE组织学的基础上,借助免疫组化染色,对于正确诊断和鉴别有重要意义。