| miR-548d抑制骨肉瘤细胞的增殖和迁移 |
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| 引用本文： | 王黎明,杨大业,仇 剑,张德巍.miR-548d抑制骨肉瘤细胞的增殖和迁移[J].现代肿瘤医学,2019,0(8):1324-1328. |
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| 作者姓名： | 王黎明 杨大业 仇 剑 张德巍 |
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| 作者单位： | 中国医科大学附属第四医院第三普通外科，辽宁 沈阳 110032 |
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| 摘 要： | 目的:探讨miR-548d对骨肉瘤细胞增殖和迁移的影响。方法:通过Real time PCR及Western blot检测miR-548d和KRAS在30例骨肉瘤组织以及293、MG63和U2OS细胞中的表达情况。对30例骨肉瘤组织中miR-548d和KRAS含量的相关性进行分析，随后通过报告基因实验印证miR-548d对KRAS的靶向作用。MG63细胞中分别过表达或沉默miR-548d后，通过Western blot实验分析KRAS的变化情况，MTT实验观察miR-548d对细胞增殖的影响，Transwell实验观察miR-548d对其迁移能力的影响。结果:骨肉瘤组织及细胞中miR-548d表达较低，KRAS表达较高。在骨肉瘤组织中miR-548d与KRAS的表达呈负相关。报告基因实验证明miR-548d可以直接打靶KRAS。Western blot指出miR-548d可以抑制KRAS的表达。最后MTT和Transwell实验指出miR-548d可以抑制MG63细胞的增殖与迁移。结论:miR-548d可以通过打靶KRAS抑制骨肉瘤细胞的增殖和迁移。
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| 关 键 词： | miR-548d 骨肉瘤 MG63 增殖 迁移 |
miR-548d inhibits the proliferation and migration of osteosarcoma cells |
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| Wang Liming,Yang Daye,Qiu Jian,Zhang Dewei.miR-548d inhibits the proliferation and migration of osteosarcoma cells[J].Journal of Modern Oncology,2019,0(8):1324-1328. |
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| Authors: | Wang Liming Yang Daye Qiu Jian Zhang Dewei |
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| Affiliation: | Third of General Surgery Department,The Fourth Affiliated Hospital of China Medical University,Liaoning Shenyang 110032,China. |
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| Abstract: | Objective:To investigate the effect of miR-548d on the proliferation and migration of osteosarcoma.Methods:The expression of miR-548d and KRAS in 30 osteosarcoma tissues and 293,MG63 and U2OS cells were detected by Real time PCR and Western blot.The correlation of the content of miR-548d and KRAS in 30 cases of osteosarcoma was analyzed,and then the target effect of miR-548d on KRAS was confirmed by the reported gene experiment.After overexpressing or silencing miR-548d in MG63 cells,the change of KRAS was analyzed by Western blot.The effect of miR-548d on cell proliferation was observed by MTT,and the effect of miR-548d on migration ability was observed by Transwell.Results:The expression of miR-548d in the tissues and cells of osteosarcoma was lower,and the expression of KRAS was higher.There was a negative correlation between the expression of miR-548d and KRAS in osteosarcoma.The report gene experiment showed that miR-548d could target KRAS directly.Western blot points out that miR-548d could inhibit the expression of KRAS.Finally,MTT and transwell showed that miR-548d could inhibit the proliferation and migration of MG63 cells.Conclusion:miR-548d can inhibit the proliferation and migration of osteosarcoma cells by targeting KRAS. |
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| Keywords: | miR-548d osteosarcoma MG63 proliferation migration |
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