Rosiglitazone down-regulates lipopolysaccharide-induced expression of CD40 and intercellular adhesion molecule 1 in rat peritoneal mesothelial cells through a NF-κB dependent mechanism

Objective To investigate the effect and mechanism by which PPARγ ligand, rosiglitasone, regulates the expression of CD40 and intercellular adhesion molecule 1 (ICAM-1) in the rat peritoneal mesothelial cells (RPMCs) induced by lipopolysaccharide (LPS). Methods RPMCs were harvested from Sprague-Dawley rat peritoneal cavity and maintained under defined in vitro conditions. The cells were randomly divided into groups as follows: medium, LPS (5 mg/L), LPS (5 mg/L)+BAY11-7085(5 μmol/L, NF-κB inhibitor), rosiglitazone (10 μmol/L or 20 μmol/L, peroxisome proliferator-activated receptor γ activator), LPS (5 mg/L)+rosiglitazone (10 μmol/L)+GW9662 (3 μmol/L, peroxisome proliferator-aetivatcd receptor γ antagonist), and LPS (5 mg/L)+vehicle (DMSO 0.2 ml/L). The expressions of CD40 and ICAM-1 RNA in RPMCs were examined by RT-PCR after 3 hour treatment, and the protein expressions of CD40, ICAM-1, p-NF-κB p65 and p-IκBα were examined by Western blot or immunofluorescence after 24 hour treatment. Results Following treatment with LPS, both the expressions of CD40 and ICAM-1 protein in RPMCs were up-regulated significantly (P<0.05), and the phosphoralation of p65 was increased greatly (1.10±0.17 vs 0.55±0.06, P<0.05). BAY11-7085 (5 μmol/L) significantly decreased the protein expression of p-p65 (0.22±0.11 vs 1.10±0.17, P<0.01), CD40 (0.34±0.02 vs 0.50±0.06, P<0.05) and ICAM-1 (0.35±0.16 vs 0.74±0.03, P<0.05). Pretreated with rosiglitazone for 3 h then added with LPS for 1 h, the levels of p-p65, CD40 and ICAM-1 in RPMCs were significantly decreased compared with those of LPS group (0.77±0.08 vs 0.90±0.10, P相似文献   

松露传奇香

意大利托斯卡纳，一座13世纪的城堡里举行了一次“香气展”，来这里参观的世界各地的游客能闻到几十种松露的香气，正如财政长官弗兰奇尼在这座松露主题博物馆的开幕式上所说，“松露对我们来说不仅仅足一种美食，它是我们文化的一部分”。就是用这样独特的香，松露打动了所有人的胃。     

防腐剂安全吗

工业食品越来越多地进入了我们的生活，防腐剂渐渐成为食品安全关注的焦点，一些过去可以使用的防腐剂渐渐被国家禁止使用，还有些防腐剂受到严格限制，那么防腐剂究竟安全还是不安全呢？     

在秋天爱上葡萄

对葡萄的描述，《神农本草经》中有这样的字句：“主筋骨湿痹，益气倍力强志，令人肥健，耐饥忍风寒，久食轻身不老延年，可作酒。”现代营养学认为，葡萄中的糖分主要是葡萄糖，能直接被人体吸收，供给身体所需能量，缓解疲劳。葡萄酒大受西方人偏爱，除去味道的独特，恐怕也是看中了它的健身功效。     

秋栗香

身居北方，深秋时节，万木枯槁，凉风拂面，少了很多乐事，唯独街边松软甜香的炒栗子屡屡勾起蠢蠢欲动的馋虫。每每站在大铁锅边看着师傅热火朝天地一阵翻炒，不多会儿滚热的栗子入手，还没吃，心里便一阵满足。     

冬天里的红柿子

“圆圆红罐罐儿，扣着圆盖盖儿，甜甜的蜜水儿，满满盛一罐儿”——打一植物名字。每当听到这个儿时的谜语就想起那些红灯笼似的柿子果，在窗台上一字排开，晒红了脸的样子。     

Rosiglitazone down-regulates lipopolysaccharide-induced expression of CD40 and intercellular adhesion molecule 1 in rat peritoneal mesothelial cells through a NF-κB dependent mechanism

Objective To investigate the effect and mechanism by which PPARγ ligand, rosiglitasone, regulates the expression of CD40 and intercellular adhesion molecule 1 (ICAM-1) in the rat peritoneal mesothelial cells (RPMCs) induced by lipopolysaccharide (LPS). Methods RPMCs were harvested from Sprague-Dawley rat peritoneal cavity and maintained under defined in vitro conditions. The cells were randomly divided into groups as follows: medium, LPS (5 mg/L), LPS (5 mg/L)+BAY11-7085(5 μmol/L, NF-κB inhibitor), rosiglitazone (10 μmol/L or 20 μmol/L, peroxisome proliferator-activated receptor γ activator), LPS (5 mg/L)+rosiglitazone (10 μmol/L)+GW9662 (3 μmol/L, peroxisome proliferator-aetivatcd receptor γ antagonist), and LPS (5 mg/L)+vehicle (DMSO 0.2 ml/L). The expressions of CD40 and ICAM-1 RNA in RPMCs were examined by RT-PCR after 3 hour treatment, and the protein expressions of CD40, ICAM-1, p-NF-κB p65 and p-IκBα were examined by Western blot or immunofluorescence after 24 hour treatment. Results Following treatment with LPS, both the expressions of CD40 and ICAM-1 protein in RPMCs were up-regulated significantly (P<0.05), and the phosphoralation of p65 was increased greatly (1.10±0.17 vs 0.55±0.06, P<0.05). BAY11-7085 (5 μmol/L) significantly decreased the protein expression of p-p65 (0.22±0.11 vs 1.10±0.17, P<0.01), CD40 (0.34±0.02 vs 0.50±0.06, P<0.05) and ICAM-1 (0.35±0.16 vs 0.74±0.03, P<0.05). Pretreated with rosiglitazone for 3 h then added with LPS for 1 h, the levels of p-p65, CD40 and ICAM-1 in RPMCs were significantly decreased compared with those of LPS group (0.77±0.08 vs 0.90±0.10, P相似文献   

机采血小板与血栓性心脑血管疾病预防的相关性探讨

目的通过成分献血（机采血小板）者与全血献血者体内血浆D－二聚体含量的比对观察,探讨二者在降低血栓性心脑血管疾病发生意义上的临床价值。方法采用全自动血凝分析透视光免疫浊度法对不同年龄段的360名健康献血者,进行体内血浆D－二聚体的含量测定,并进行比对观察。结果180名成分献血（机采血小板）者体内D－二聚体的含量显著低于180名全血献血者,2组献血者不同年龄血浆D－二聚体含量差异均有统计学意义（P〈0．01）。结论在降低血栓性疾病发生意义上,成份献血更优于传统的全血献血。     

石榴皮总黄酮的微波提取工艺及含量测定研究

目的：研究石榴皮中总黄酮的微波提取最佳工艺条件并测定其含量。方法：采用微波提取法提取石榴皮中总黄酮,通过单因素试验,考察了微波功率、乙醇体积分数、料液比、微波提取时间4个因素对总黄酮提取的影响,通过正交试验进一步优化提取工艺,运用硝酸铝显色法测定总黄酮含量。结果：微波提取法提取石榴皮中总黄酮的最佳工艺为乙醇体积分数60%,料液比为1∶25,微波提取时间为120 s;微波功率对总黄酮提取无显著影响,其余3个因素对总黄酮提取的影响依次为乙醇体积分数＞料液比＞微波提取时间;总黄酮平均含量为6.70%。结论：采用微波提取法提取石榴皮中的总黄酮,具有快速、高效、节能、不破坏有效成分等特点,具有良好的应用前景。     

黄芪甲苷对糖尿病KKAy小鼠肾组织TGF-β1、SMAD2/3及α-SMA表达的影响

目的 观察黄芪甲苷对糖尿病KKAy小鼠肾组织转化生长因子β1(TGF-β1)、SMAD2/3、q-平滑肌肌动蛋白(α-SMA)表达的影响,探讨其延缓肾脏纤维化的可能机制.方法 雄性C57BL/6J小鼠10只作为对照组,20只2型糖尿病模型KKAy小鼠予以高脂饮食至14周,随机数字法分为模型组和黄芪甲苷组,每组10只,黄芪甲苷组给予黄芪甲苷40 mg·kg-·d-1,模型组与对照组给予等量生理盐水.实验期间,各组动物自由饮食、饮水.血糖仪测量16、20、24周龄时各组小鼠的血糖水平.24周时处死,观察各组小鼠肾的病理学变化,免疫组织化学法测定TGF-β1、SMAD2/3、α-SMA的表达.结果 (1)血糖:与对照组相比,14周龄的KKAy小鼠血糖明显升高,模型组血糖在16、20、24周时血糖明显升高(P＜0.05),黄芪甲苷组与其相比,血糖下降(P＜0.05).(2)肾组织形态学:对照组肾小球及肾小管结构清晰,未出现肾间质纤维化,模型组肾小球系膜基质增宽,系膜细胞增多,肾小管上皮细胞细胞质空泡样变性,肾间质炎性细胞增多,黄芪甲苷组肾小管上皮细胞细胞质较少,未呈现明显的纤维化.(3)肾组织TGF-β1、SMAD2/3、α-SMA的表达:对照组TGF-β1表达微弱,而模型组TGF-β1显著表达于肾小管上皮细胞细胞质(P＜0.01);与模型组相比,黄芪甲苷组TGF-β1、α-SMA表达明显下调(P＜0.05);对照组肾小管与肾小球细胞核有少量磷酸化的SMAD2/3表达,模型组表达增加(P＜0.01),与模型组相比,黄芪甲苷组表达减少(P＜0.05).结论 黄芪甲苷通过影响TGF-β/SMADS信号通路,下调TGF-β1、α-SMA表达,改善糖尿病小鼠肾脏纤维化.