Effects of penetrating cornea injury combined with seawater immersion on the levels of aqueous humor TNF-α in rabbits

Objective To observe effects of penetrating cornea injury combined with seawater immersion on the levels of aqueous humor TNF-α in rabbits. Methods 10 adult healthy grey rabbits were used for the experiment. The rabbit eye model of comea blast injury was developed by using a single firecracker. For each rabbit, the right eye was the experimental eye (seawater was injected into the anterior chamber through the cornea incision, simulating seawater immersion) and the left eye was the control (treated with 0.9% sodium chloride). Levels of aqueous humor TNF-α were detected by using double-antibody sandwich ELISA on the 1st, 2nd, yrd, 4th, 5th, and 7th days following seawater immersion or 0.9% sodium chloride injection. Results Following development of the model, levels of aqueous humor TNF-α in the experimental eye on the 1st, 2nd, 3rd days were 190.338, 156. 107,117.018 ng/L respectively, and significant differences could be seen when comparisous were made between the experimental and control eyes. The level of aqueous humor TNF-α on the 1st day was the highest and obviously reached the peak. Conclusions Level of aqueous humor TNF-α in the experimental eye with penetrating cornea injury combined with seawater immersion was significantly higher than that of the control eye, indicating that TNF-α was clearly involved in the pathological change and it played an important role in the process.     

尿激酶溶栓治疗急性心肌梗塞过程中血清肌红蛋白的变化

目的：检测急性心肌梗塞患者尿激酶溶栓治疗过程中血清肌红蛋白（ｍｙｏｇｌｏｂｉｎ, Ｍｂ）的动态变化。方法：应用ＥＬＩＳＡ方法检测血清Ｍｂ的水平。结果：再灌注组血清Ｍｂ的峰浓度（６１９．５９±１９８．５５ｎｇ／ｍｌ）显著高于未再灌注组（４８７．３１±６０．８７ｎｇ／ｍｌ）,而且再灌注组较未再灌注组血清Ｍｂ达峰时间明显提前（１９２ｍｉｎ对４８０ｍｉｎ,Ｐ＜０．０１）,再灌注组Ｍｂ的２小时出现率（Ｍｂ２／Ｍｂ０）较未再灌注组也显著增高（４．２４对２．１２,Ｐ＜０．０１）。结论：血清Ｍｂ值在尿激酶溶栓治疗冠脉再灌注后早期上升、快速达峰,可以作为一种可靠而简便的判断ＡＭＩ再灌注治疗成功与否的指标。     

CEUS观察兔肝VX2肿瘤坏死演变

目的应用CEUS技术观察兔肝VX2肿瘤坏死过程,探讨VX2肿瘤生长坏死规律及其对实验产生的影响。方法应用超声引导下经皮穿刺种植法成功建立24只兔VX2肝肿瘤模型,并通过CEUS技术分别在肿瘤种植后10、14、21、28天动态观察肝肿瘤演变过程,测量肿瘤体积及坏死体积,计算瘤内坏死率。结果在肿瘤种植后10、14、21、28天兔肝VX2肿瘤体积、坏死灶体积和坏死率比较,差异均有统计学意义(P均0.05);肿瘤种植后10、14、21、28天的CEUS表现能反映出肿瘤的病理学特征。实验依据CEUS和病理特点将肿瘤生长过程细分为5个时期,0~10天为肿瘤实质前期;11~14天为肿瘤实质后期;15~21天为肿瘤坏死初期;22~28天为加速坏死期;28天后为肿瘤囊变期。结论 CEUS可准确判断肝肿瘤实质及坏死范围;VX2肿瘤的生长演变及肿瘤坏死具有规律性,可根据不同实验要求选择适宜的研究时间段。     

手术护理路径在经尿道等离子前列腺手术配合中的应用

目的 观察经尿道等离子前列腺手术中应用手术护理路径的效果。方法 将接受尿道等离子前列腺手术的45例患者随机分为2组,对照组20例患者接受常规护理,观察组25例患者接受手术护理路径护理,对2组患者的住院时间、护理不良事件发生情况以及健康教育知识掌握情况进行比较分析。结果 对照组患者住院时间为（10.13±1.64）d,观察组为（8.71±1.58）d;护理不良事件发生率对照组为15.0%,观察组为4.0%;健康知识评分对照组为23.48±1.47,观察组为27.86±1.45;2组患者3项指标比较,差异均有统计学意义（P〈0.05）。结论 经尿道等离子前列腺手术中应用手术护理路径较常规护理能缩短住院时间,降低不良事件发生率。     

24h动态血压非规律变化与左心室肥厚的相关性分析

长期以来高血压对心血管系统的影响主要依据血压的高低来估测心血管疾病的危险性，近年来亦有根据脉压的大小来预测心血管事件。本文通过对高血压患者进行24h动态血压监测和超声心动图检测，探讨血压非规律变化与左心室肥厚的相关性，以便早期发现及预防高血压左心室肥厚的发生。     

硫酸镁治疗支气管哮喘20例疗效观察

自1996年元月以来．我们应用硫酸镁治疗支气管哮喘．取得了满意效果．现报道如下。     

眼部结核2例

1病例报告例1:女,59岁,住院号304409,退休职员。患者发右上睑外侧包块1+mo,包块渐增大,无疼痛、复视、眼球动障碍,无发热、咳嗽、盗汗等。27a前因左眼新生血管青光眼,行左眼球摘除术。入院查体:右眼视力1.0,上外侧饱满,皮肤无明显充血,眼眶外上方触及条索状包块大小约1cm×0.5cm,后界不清,睑结膜面未触及,包块中,无明显压痛,眼球活动度正常,屈光介质透明,眼底见异常。左眼球缺如。颌下、耳前淋巴结未触及,心、肺腹部检查无明显阳性体征。眼眶CT报告:右侧泪腺区见不规则软组织密度影,与右侧眼球分界欠清晰,其内度尚均匀,CT值约32Hu,其大小约0.8cm×2cm,眶壁见骨质破坏。入院     

Effects of penetrating cornea injury combined with seawater immersion on the levels of aqueous humor TNF-α in rabbits

Objective To observe effects of penetrating cornea injury combined with seawater immersion on the levels of aqueous humor TNF-α in rabbits. Methods 10 adult healthy grey rabbits were used for the experiment. The rabbit eye model of comea blast injury was developed by using a single firecracker. For each rabbit, the right eye was the experimental eye (seawater was injected into the anterior chamber through the cornea incision, simulating seawater immersion) and the left eye was the control (treated with 0.9% sodium chloride). Levels of aqueous humor TNF-α were detected by using double-antibody sandwich ELISA on the 1st, 2nd, yrd, 4th, 5th, and 7th days following seawater immersion or 0.9% sodium chloride injection. Results Following development of the model, levels of aqueous humor TNF-α in the experimental eye on the 1st, 2nd, 3rd days were 190.338, 156. 107,117.018 ng/L respectively, and significant differences could be seen when comparisous were made between the experimental and control eyes. The level of aqueous humor TNF-α on the 1st day was the highest and obviously reached the peak. Conclusions Level of aqueous humor TNF-α in the experimental eye with penetrating cornea injury combined with seawater immersion was significantly higher than that of the control eye, indicating that TNF-α was clearly involved in the pathological change and it played an important role in the process.     

当归活性成分阿魏酸钠干预老年冠状动脉粥样硬化性心脏病心绞痛患者血液抗氧化能力和保护血内皮细胞功能

目的：观察中药当归提取的主要活性成分阿魏酸钠对老年冠状动脉粥样硬化性心脏病（简称冠心病）心绞痛患者血浆及红细胞中过氧化脂质含量和超氧化物歧化酶活力、全血谷胱甘肽过氧化物酶活性、红细胞过氧化氢酶活性、血浆内皮素和一氧化氮水平的影响。 方法：①选择2004—06／2005—06吉林大学第一医院老年病科住院老年冠心病心绞痛患者42例（男35例，女7例）为治疗组。年龄61～77岁。均按国际心脏病学会和及世界卫生组织制定的缺血性心脏病的命名和诊断标准诊断为冠心病心绞痛。选择同期本院年度进行健康体检人员40名男性为对照组，年龄58～76岁。纳入对象均对检测指标知情同意。治疗组给予阿魏酸钠注射液[巴里莫尔制药（通化）有限公司，生产批号20060501]300mg加入50g／L葡萄糖注射液250mL，静脉滴注，1次／d，疗程2周。②治疗组患者于治疗前和治疗2周末，对照组于约定日，采用硫代巴比妥酸显色法测定血浆及红细胞中过氧化脂质，采用连苯三酚自氧化法测定血浆及红细胞超氧化物歧化酶活性，采用酶动力学法测定红细胞过氧化氢酶活性。采用酶偶联分光光度法测定全血谷胱甘肽过氧化物酶活性。采用硝酸还原法测定血浆一氧化氮水平，采用放射免疫测定血浆内皮素水平。 结果：老年冠心病心绞痛患者42例和体检者40名均进入结果分析。①阿魏酸钠的清除氧自由基能力：治疗组治疗前血浆和红细胞过氧化脂质含量明显高于对照组（P＜0.01），血浆和红细胞超氧化物歧化酶活性、红细胞过氧化氢酶和谷胱甘肽过氧化物酶活性明显低于对照组（P＜0．01）。治疗组治疗后血浆和红细胞过氧化脂质含量明显低于治疗前（P＜0,05），血浆和红细胞超氧化物歧化酶活性、红细胞过氧化氢酶和谷胱甘肽过氧化物酶活性明显高于治疗前（P＜0．05～0．01）。说明阿魏酸钠有清除自由基、减轻膜脂质过氧化、提高抗氧化酶活性的作用。②阿魏酸钠对血管内皮功能的影响：治疗组治疗前血浆一氧化氮水平为（21.1&;#177;11．7）μmol／L，低于对照组[（36、4&;#177;10．2）μmol／L，P＜0.01]，血浆内皮素水平为（94．4&;#177;16．9）ng／L，高于对照组[（51．8&;#177;17．7）．g／L，P＜0．01]。治疗组治疗后血浆一氧化氮水平为（34．7&;#177;10．9）μmol／L，高于治疗前（P＜0．01），血浆内皮素水平为（65．4&;#177;17．4）ng／L，低于治疗前（P＜0．01）。 结论：阿魏酸钠干预老年冠心病心绞痛患者具有清除自由基、减轻膜脂质过氧化、提高抗氧化酶活性的作用；同时可降低血浆内皮素水平，升高一氧化氮水平，从而改善血管内皮功能。     

临床常见诊疗错误汇编(186)

CT检查虽可贵临床表现价更高--1例延髓及小脑梗死的误诊启示 男,41岁,因头晕20天伴左侧肢体活动不灵3天入院.患者于入院前20天在行车中突发头晕,摔倒,并出现反复呕吐、呕吐物为胃内容物,急送当地医院检查,经头颅CT扫描未见异常,给予对症处理,转入我院3天前自觉左侧肢体活动不灵,手足麻木,发凉并头晕加重,而转我院治疗.查体:神清、体温37C,心率70次/分,呼吸20次/分,血压25/16 kPa,双侧瞳孔等大等圆,对光反射存在,双眼底动脉硬化Ⅰ度.口角无歪斜,鼻唇沟无变浅,伸舌居中,心、肺、腹(一).左上肢肌力Ⅳ级,左下肢肌力Ⅲ级,右侧肢体肌力正常,左侧肢体痛、温觉减弱.实验室检查血常规,血糖、血尿素氮,肝功能,尿、粪常规均正常.