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目的:本文通过Rnt1p对细胞周期相关mRNAs的作用来探讨RNA的稳定性与细胞周期中细胞壁应激依赖调控之间的关系。方法:用定量RT-PCR分析Rnt1 mRNA的表达水平;用酵母细胞的免疫荧光标记检测Swi4p-和Hsl1p-GFP的融合蛋白;用扫描式分光光度计检测细胞的生长过程。结果:1)酵母细胞核Rnt1p直接裂解细胞周期调控相关基因的mRNAs;2)RNT1的缺失改变了Hsl1p水平和细胞的定位;3)HSL1的过表达引起RNT1缺失表型的部分复制;4)HSL1的缺失部分地抑制了rnt1△细胞表型的缺陷。在这些基因调控模型中细胞核mRNA的降解加强了细胞应激反应与细胞周期之间的连系。结论:细胞核mRNA的选择性降解不仅需要细胞壁的应激反应也需要细胞壁完整性检查点的调控,酵母细胞核Rnt1p的缺失增加了和形态发生检查点及细胞壁整体通路都相关的mRNAs表达,最终导致应激反应的减弱。 相似文献
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目的观察用β-氨基丙腈(β-aminopropionitrile,BAPN)抑制赖氨酰氧化酶(lysyl oxidase,LOX)对荷胃癌裸鼠肿瘤形成和转移的影响。方法 6周龄的裸鼠,随机分为BAPN预处理组和对照组,预处理组10只,对照组15只。BAPN预处理组每天用BAPN 100mg.kg-1腹腔注射,对照组每天用相同体积的无菌PBS腹腔注射。2周后,以人胃癌细胞SGC-7901 1×107.mL-1建立荷胃癌裸鼠模型。肿瘤细胞接种4周后,比较成瘤率、瘤重和转移率。结果 BAPN预处理组平均瘤重高于对照组,P<0.05;BAPN预处理组的成瘤率和转移率与对照组的差异无统计学意义。结论用BAPN预先抑制LOX活性后,肿瘤的重量明显增加,但对原发肿瘤形成和转移未见明显作用。 相似文献
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Objective To study the effects of lysyl oxidase ( LOX) on the migration and adhesion of the human gastric cancer cell line HGC-27 cells in vitro. Methods The human gastric cancer cell line HGC-27 cells were cultured in vitro, and treated with different concentration of β-aminopropionitrile (BAPN). The ability of migration was assessed by wound-healing assay. The ability of adhesion was detected by homogenous and heterogeneous adhesion experiments. Results Compared that with 0 mmol/L BAPN, the ability of migration of the cells after treatment with 0.2 mmol/L BAPN was descended at 8, 24, 32 and 48 h; the number of cells with homogeneous adhesion was increased from (6.97 ± 0.07) x l03/ml to (7.78±0.11) ×103/ml; and the number of cells with heterogeneous adhesion was decreased from (8.98± 0. 15) x 103/ml to (8. 35 ±0. 10) ×103/ml, both <0. 05. Compared with that of cells treated with 0 mmol/L and 0.2 mmol/L BAPN, the migration ability of cells after treatment with 0.3 mmol/L BAPN was descended at 8, 24, 32 and 48 h; the number of cells with homogeneous adhesion was raised to (8. 02 ± 0. 11) ×103/ml and the number of cells with heterogeneous adhesion was down to (7.93 ±0.07) ×103/ml (P < 0. 05). Conclusion LOX may promote the metastasis of cancer cells by enhancing invasion, increasing heterogeneous adhesion and decreasing homogeneous adhesion. 相似文献
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Objective To study the effects of lysyl oxidase ( LOX) on the migration and adhesion of the human gastric cancer cell line HGC-27 cells in vitro. Methods The human gastric cancer cell line HGC-27 cells were cultured in vitro, and treated with different concentration of β-aminopropionitrile (BAPN). The ability of migration was assessed by wound-healing assay. The ability of adhesion was detected by homogenous and heterogeneous adhesion experiments. Results Compared that with 0 mmol/L BAPN, the ability of migration of the cells after treatment with 0.2 mmol/L BAPN was descended at 8, 24, 32 and 48 h; the number of cells with homogeneous adhesion was increased from (6.97 ± 0.07) x l03/ml to (7.78±0.11) ×103/ml; and the number of cells with heterogeneous adhesion was decreased from (8.98± 0. 15) x 103/ml to (8. 35 ±0. 10) ×103/ml, both <0. 05. Compared with that of cells treated with 0 mmol/L and 0.2 mmol/L BAPN, the migration ability of cells after treatment with 0.3 mmol/L BAPN was descended at 8, 24, 32 and 48 h; the number of cells with homogeneous adhesion was raised to (8. 02 ± 0. 11) ×103/ml and the number of cells with heterogeneous adhesion was down to (7.93 ±0.07) ×103/ml (P < 0. 05). Conclusion LOX may promote the metastasis of cancer cells by enhancing invasion, increasing heterogeneous adhesion and decreasing homogeneous adhesion. 相似文献
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目的 比较两株胃癌细胞HGC-27与SGC-7901的体外增殖和黏附能力.方法 分别培养胃癌细胞HGC-27、SGC-7901,用噻唑蓝(MTT)比色实验和流式细胞术(FCM)比较两种细胞的体外增殖能力;用同质黏附和异质黏附实验比较两种细胞的体外黏附能力.结果 MTT比色实验绘制,生长曲线显示SGC-7901生长较HCC-27慢;流式细胞术结果表明SGC-7901的增殖活性低于HGC-27(P<0.05);黏附实验中SGC-7901同质黏附强于HGC-27,异质黏附弱于HGC-27(P<0.05).结论 胃癌细胞SGC-7901的体外增殖能力和异质黏附能力低于HGC-27,同质黏附能力高于HGC-27,提示胃癌细胞HGC-27的转移能力可能强于SGC-7901. 相似文献
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