HIF-1和BFGF、BFGFR与大肠癌的关系

缺氧诱导因子-1（Hypoxia inducible factor-1，HIF-1）是缺氧条件下广泛存在于哺乳动物和人体内的一种转录因子。大量的研究表明HIF-1在大肠癌组织中高表达，且与肿瘤的多种生物学行为有相关性，说明HIF-1在大肠癌的发病机制中扮演着一个相当重要的角色。细胞生长因子与肿瘤的关系已日益受到重视。多种生长因子在不同的肿瘤组织或体液中可被检测；多种细胞生长因子的受体在某些肿瘤组织中高表达；     

COX-2 和BFGF 、BFGFR 在大肠癌中的表达及其与Dukes 分期、淋巴结转移的关系

 目的 研究COX-2、BFGF、BFGFR在大肠癌中的表达和相互作用,以及它们与大肠癌Dukes分期、淋巴结转移之间的关系。方法 采用免疫组化SP法检测了49例手术切除的大肠癌及20例大肠增生性息肉组织中的COX-2、BFGF、BFGFR表达。结果 COX-2、BFGF、BFGFR在大肠癌中的表达阳性率分别为59.2%、69.3%、65.3%。而增生性息肉的表达率分别为30.0%、40.0%、35.0%。COX-2、BF-GF、BFGFR在大肠癌组织与增生性息肉中表达差异有统计学意义（P〉0.05）。COX-2、BFGF、BFGFR在大肠癌中表达与肿瘤Dukes分期、淋巴结转移之间差异有统计学意义（P〈0.05）。大肠癌组织中COX-2与BFGFR无相关性（P〉0.05）,但COX-2与BFGF,BFGF与BFGFR具有相关性（P〈0.05）。结论 大肠癌组织中的COX-2、BFGF、BFGFR表达水平增高,参与了大肠癌的发生发展过程,与大肠癌的预后有关;在大肠癌发生发展中COX-2与BFGFR可能是相互独立的作用因子,但COX-2与BFGF,BFGF与BFGFR可能起到协同作用,共同促进肿瘤的发生。     

学生因考试应激引起急性上消化道出血25例分析

目的了解青年学生在应考前、后发生急性上消化道出血的病因。方法对25例应考学生急性上消化道出血的临床资料进行分析。结果25例患者均为在考试期间由于紧张、焦虑、疲劳、睡眠不足甚至失眠和饮食失调,从而导致心身两方面产生过度应激反应,引起上消化道出血,经内科保守治疗,临床治愈。结论学生在考试期间因紧张,使心身处于严重的应激状态,此时生理代谢功能不足以维持胃黏膜微循环正常运行,引起胃黏膜屏障结构遭到破坏而导致出血。     

逆行胰胆管造影(ERCP)及十二指肠乳头切开术(EST)术中的配合及观察

目的:探讨逆行胰胆管造影(ERCP)及十二指肠乳头切开术(EST)术中医护人员的配合及观察。方法:对52例患有胆道疾病或胰腺疾病患者行逆行胰胆管造影检查及十二指肠乳头切开术。结果:52例患者中其中有38例成功完成了手术。结论:逆行胰胆管造影检查及十二指肠乳头切开术是消化内镜手术的一部分,属于介入和微创手术,对多数胆系疾病或胰腺疾病的诊断和治疗有重要价值,而手术中的医护人员默契配合是手术成功的关键。     

结直肠腺癌组织中HIF-1α与BFGFR的表达

目的 研究缺氧诱导因子1α(HIF-1α)和碱性成纤维牛长因子受体(BFGFR)在结直肠腺癌中的表达和相瓦作用,以及它们与结直肠腺癌牛物学行为的关系.方法 采用免疫组化SP法检测了60例手术切除的结直肠腺癌及癌旁组织、20例正常大肠黏膜组织中的HIF-1α和BFGFR表达.结果 HIF-1 α和BFGFR在结直肠腺癌中的表达阳性率分别为61.7%和58.3%,明显高于癌旁组织的10.0%和11.7%(P＜0.05).正常肠黏膜中未检出HIF-1α、BFGFR,HIF-1α和BFGFR在结直肠腺癌中表达与性别、年龄、肿瘤大小、肿瘤位置和分化程度无关(P＞0.05),但与肿瘤Dukes分期相关(P＜0.05).结直肠腺癌组织中HIF-1α与BFGFR表达具有相关性(P＜0.05).结论 结直肠腺癌组织中的HIF-1α和BFGFR表达水平增高,参与了结直肠腺癌的发生发展过程;且HIF-1α与BFGFR可能起到协同作用,共间促进肿瘤的发生.     

内镜下高频电凝联合根除幽门螺杆菌治疗隆起糜烂性胃炎的临床研究

目的 探讨应用内镜下高频电凝联合根除幽门螺杆菌(Hp)治疗隆起糜烂性胃炎(EGP)的价值和安全性.方法 将60例EGP患者分成A组(抑酸、保护胃黏膜、根除Hp药物治疗)和B组(抑酸、保护胃黏膜、根除Hp药物+高频电凝治疗同时进行)各30例,比较两种方法 的疗效.结果 A组单纯药物治疗后临床症状缓解(P＜0.05),但隆起病灶数消失不明显,仅有18.57%(47/253)的隆起病灶消失;B组治疗后,临床症状明显缓解(P＜0.05),97.15%(273/281),隆起病灶消失(P＜0.05),与单纯药物治疗组比较差异有统计学意义(P＜0.05).有6例出现轻微上腹胀或上腹隐痛,均在术后5 d内消失.结论 EGP单纯药物治疗疗效欠佳,内镜下高频电凝联合根除Hp治疗EGP不仅疗效确切,而且安全简便.     

Expression of hypoxia inducible factor-1α and basic fibroblast factor in the tissues of colorectal adenocarcinoma

Objective To observe expression of hypoxia inducible factor-1α(HIF-1α)and basic fibroblast growth factor(BFGF)in the tissues of colorectal adenoearcinoma and analyze the relationship between expression of the two factors and biological behavior of colorectal adenocarcinoma.Methods The samples of colorectal adenocarcinoma(n=60)and para-adenocarcinoma(n=60)were taken from surgical resection patients and normal colorectal tissues (n=20) from patients with irritable bowel syndrome.The expression of HIF-1α and BFGF were detected by immunohistochemical staining (SP method).Results Positive rates of HIF-1αand BFGF in colorectal adenocarcinoma tissues were 61.7% and 65.0%,and positive rates of HIF-1α and BFGF in para-adenocarcinoma tissues were 10.0%and 13.3%(P<0.05,respectively);HIF-1αand BFGF were not detected in normal intestinal mucosa.There was no significant correlation with HIF-1α and BFGF expression in colorectal adenocarcinoma tissues to the sexuality,age,tumor size,tumorposition and differentiation(P>0.05).A significant correlation between expression of the two factors and Dukes stage was observed (P<0.05).Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissuesof Dukes A and B stage were 47.2%and 52.7%,respectively.Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissues of Dukes C and D stage were 83.3%and 83.3%.respectively.There was a positive correlation between expression of the two factors and carcinogenesis of colorectal adenocarcinoma(r=0.4276.P<0.001).Conclusion The results showed that the enhanced expression of HIF-1 α and BFGF incolorectal adenocarcinoma tissues may be associated with the prognosis of the patients with colorectal adenocarcinoma,and HIF-1α and BFGF may participate synergistically in the carcinogenesis of colorectal adenocarcinoma.     

Expression of hypoxia inducible factor-1α and basic fibroblast factor in the tissues of colorectal adenocarcinoma

Objective To observe expression of hypoxia inducible factor-1α(HIF-1α)and basic fibroblast growth factor(BFGF)in the tissues of colorectal adenoearcinoma and analyze the relationship between expression of the two factors and biological behavior of colorectal adenocarcinoma.Methods The samples of colorectal adenocarcinoma(n=60)and para-adenocarcinoma(n=60)were taken from surgical resection patients and normal colorectal tissues (n=20) from patients with irritable bowel syndrome.The expression of HIF-1α and BFGF were detected by immunohistochemical staining (SP method).Results Positive rates of HIF-1αand BFGF in colorectal adenocarcinoma tissues were 61.7% and 65.0%,and positive rates of HIF-1α and BFGF in para-adenocarcinoma tissues were 10.0%and 13.3%(P<0.05,respectively);HIF-1αand BFGF were not detected in normal intestinal mucosa.There was no significant correlation with HIF-1α and BFGF expression in colorectal adenocarcinoma tissues to the sexuality,age,tumor size,tumorposition and differentiation(P>0.05).A significant correlation between expression of the two factors and Dukes stage was observed (P<0.05).Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissuesof Dukes A and B stage were 47.2%and 52.7%,respectively.Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissues of Dukes C and D stage were 83.3%and 83.3%.respectively.There was a positive correlation between expression of the two factors and carcinogenesis of colorectal adenocarcinoma(r=0.4276.P<0.001).Conclusion The results showed that the enhanced expression of HIF-1 α and BFGF incolorectal adenocarcinoma tissues may be associated with the prognosis of the patients with colorectal adenocarcinoma,and HIF-1α and BFGF may participate synergistically in the carcinogenesis of colorectal adenocarcinoma.     

Expression of hypoxia inducible factor-1α and basic fibroblast factor in the tissues of colorectal adenocarcinoma

Objective To observe expression of hypoxia inducible factor-1α(HIF-1α)and basic fibroblast growth factor(BFGF)in the tissues of colorectal adenoearcinoma and analyze the relationship between expression of the two factors and biological behavior of colorectal adenocarcinoma.Methods The samples of colorectal adenocarcinoma(n=60)and para-adenocarcinoma(n=60)were taken from surgical resection patients and normal colorectal tissues (n=20) from patients with irritable bowel syndrome.The expression of HIF-1α and BFGF were detected by immunohistochemical staining (SP method).Results Positive rates of HIF-1αand BFGF in colorectal adenocarcinoma tissues were 61.7% and 65.0%,and positive rates of HIF-1α and BFGF in para-adenocarcinoma tissues were 10.0%and 13.3%(P<0.05,respectively);HIF-1αand BFGF were not detected in normal intestinal mucosa.There was no significant correlation with HIF-1α and BFGF expression in colorectal adenocarcinoma tissues to the sexuality,age,tumor size,tumorposition and differentiation(P>0.05).A significant correlation between expression of the two factors and Dukes stage was observed (P<0.05).Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissuesof Dukes A and B stage were 47.2%and 52.7%,respectively.Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissues of Dukes C and D stage were 83.3%and 83.3%.respectively.There was a positive correlation between expression of the two factors and carcinogenesis of colorectal adenocarcinoma(r=0.4276.P<0.001).Conclusion The results showed that the enhanced expression of HIF-1 α and BFGF incolorectal adenocarcinoma tissues may be associated with the prognosis of the patients with colorectal adenocarcinoma,and HIF-1α and BFGF may participate synergistically in the carcinogenesis of colorectal adenocarcinoma.     

AIDS合并马尔尼菲青霉病患者外周血Dectin-1、炎症细胞因子及CD4+T淋巴细胞表达情况及其临床意义

目的 探讨AIDS合并马尔尼菲青霉菌病(PsM)患者模式识别受体Dectin-1、炎性细胞因子及CD4+T淋巴细胞表达情况及其临床意义.方法 将120例AIDS患者根据是否合并PsM分为PsM组和非PsM组各60例,比较两组Dectin-1的平均荧光强度、CD4+T淋巴细胞计数和白细胞介素(IL)-2、IL-6、IL-12、IL-23水平.结果 PsM组的Dectin-1表达、CD4+T淋巴细胞计数、IL-2、IL-6、IL-12 和IL-23水平均低于非PsM组(P<0.05).结论 Dectin-1及其调节产生的炎症细胞因子、CD4+T水平下降可能是导致AIDS的患者对马尔尼菲青霉菌易感的重要原因.