固定液种类和固定时间对免疫组化染色的石蜡切片中抗原检测…

采用３种不同的固定液及固定时间，分别对３种含有不同抗原的组织进行固定，应用石蜡切片免疫组化染色比较他们的结果。发现：固定时间以６－１２ｈ最佳。而检测不同抗原应选择不同的固定液，检测Ｂ淋巴细胞Ｌ２６抗原，应首选Ｂ－５固定液，检测Ｖｉｍｅｎｔｉｎ及ＥＨＦＶ抗原以１０％ＮＢＦ最佳。     

人GFP—AWP1融合基因载体的构建及其在293细胞中的表达

目的 克隆、构建绿色荧光蛋白(GFP)-AWPI(associated with protein kinase Crelated kinase I，AWPI)表达载体,观察AWPI在293细胞中表达和定位。方法 采用逆转录PCR(RT—PCR)法从人ECV304内皮细胞中扩增AWPI cDNA编码区．并将其重组于GFP表达载体pEGFP—C2中。经酶切，序列鉴定分析后，将该重组质粒通过DOTAP脂质体介导，转染293细胞。荧光显微镜观察AWPI在细胞内的表达和分布。结果 GFP—AWPI融合基因表达载体经酶切鉴定和测序分析确认构建成功，并在293细胞中获得了高效表达。荧光显微镜下，在不携带外源基因的空载体pEGFP—C2转染的对照组293细胞,绿色荧光均匀分布于整个细胞中；在重组质粒pEGFP—C2／AWPI转染的293细胞，绿色荧光弥散分布于细胞质内。结论 成功构建GFP—AWPI融合基因表达载体并表达于293细胞胞质中。     

一种可显示两种不同类型胶原的双重免疫组化染色

采用ＡＢＣ法（或ＰＡＰ法）和ＳＡＢＣ法的免疫酶双重标记染色法，能在同一张组织切片上先后显示，同时观察两种不同类型胶原成分。其反应生成物颜色鲜艳，对比度好，尤其适用于观察同一病变组织中不同类型胶原的分类及含量的变化，对比较观察极为有利。     

P^16蛋白和PCNA在肝细胞性肝癌中的表达及意义

探讨肝细胞肝性癌（ＨＣＣ）中抑癌基因Ｐ＾１６蛋白及增殖细胞核抗原（ＰＣＮＡ）的表达,相关性及其与肿瘤临床病理的关系。方法：采用免疫组化ＳＰ法检测４５例ＨＣＣ及癌帝肝组织中Ｐ＾１６蛋白及ＰＣＮＡ。结果：①Ｐ＾蛋白及ＰＣＮＡ在ＨＣＣ中的阳性表达率分别明显低于,高于癌旁肝组织。     

CS-2微机控制多点温度、杀菌强度F值自动测试仪通过部级鉴定

CS-2自动测试仪(亦称CS2温度信息处理机)的研制系轻工部下达国家科技六五攻关项目“芦笋罐头高效生产线”的一个子项目,编号为(65)8-(7)。该子项目由无锡轻工业学院自动化系部分教师承担。所研制仪器与生产线杀菌工段配合使用。经过一年多时间的努力,     

INDUCTION OF LUNG CARCINOMA BY INTRALOBAR BRONCHIAL INSTILLATION OF IODIZED OIL IN RATS

This paper reports a method for experiment- al induction of bronchogenic carcinoma by intra- lobar-bronchialinstillationof carcinogenic iodized oil in rats. The animals used in the study were divided int0 4 groups, ea.ch given different dosages. The 3-methylcholanthrene (MCA) or MCA and diethyinitrosamine (DEN) were dis- solved in iodized oil. The results show that 49 0f the 57 Group I outbred rats developed bronchogenic squamous cell ca.rcinoma within 240 days, a tumor induc- tion rate of 85.970. All 25 standard Wista.r rats of Group II developed the same type of carcinoma in 80 days, the tumor induction rate being 10070. The 35 standard Wistar rats of Group III developed bronchogenic carcinoma in 22 days, with a tumor induction rate o.f lOOTo. No respiratory tract tumors were found in any of the 40 control animals of Group IV. The roentgenographic results coincided with the gross pathologic examination. The advantages of this method are: the instilled carcinogen loca.lizes in l or 2 lobes with little injury to lung tissue and oinly slight irrita- tion to the respiratory function; because the carcinogen is injected deeply into the bronchioli respiratorii or ductuli alveolares it acts w'ith sufficient effectiveness without any oil rejection by the animal; carcinogen instillation is re- quired only once or twice; and the iodized oil plays a dual role as liposolvent and contrast medium, the former action dissolving the carcinogen into its more effective form and the latter being good for ro,entgenography. Results as observed in Group III probaly better for rapid development of lung carcinoma in Wistar rats.     

宫颈癌前病变和宫颈癌组织中凋亡相关基因survivin的表达及其与人乳头状瘤病毒感染的关系

目的探讨凋亡相关基因survivin在宫颈癌前病变和宫颈癌组织中的表达及其与人乳头状瘤病毒（HPV）感染的相关性.方法选取76例宫颈癌前病变或宫颈癌患者,另取10例正常宫颈组织作为对照.应用免疫组织化学SP法检测各组宫颈组织中凋亡相关基因survivin的表达;设计高危型HPV（HPV16,18,31,33,58等）通用引物,以touch-down PCR法检测各组宫颈组织中高危型HPV的感染率.结果（1）从正常宫颈→癌前病变→宫颈癌,survivin表达逐渐增强（P＜0.05）;（2）survivin表达与宫颈癌组织学分级有关（P＜0.05）,而与年龄、临床分期及组织学分型无关（P＞0.05）;（3）从正常宫颈→癌前病变→宫颈癌,高危型HPV阳性率逐渐升高（P＜0.05）;（4）高危型HPV感染率在不同年龄、临床分期、组织学分级及组织学分型中,均无统计学差异（P＞0.05）;（5）survivin与高危型HPV在宫颈癌组织中的表达呈正相关（P＜0.05）.结论survivin表达及高危型HPV感染可能与宫颈癌的发生、发展密切相关,两者在宫颈癌的发病机制中可能起着协同作用.