Effects of 5-Hydroxytraptamine on Aggregation,Release Reaction and Mobilization of Cytosolic Free Calcium Concentration in Rabbit Normal and Washed Single Platelets

In rabbit platelet rich plasma (PRP), 5-hydroxytraptamine (5-HT,0.03～3μmol/L) induced decrease in light transmission (DLT) dose-de pendently with centralization, as revealed by electron microscopy. However, 5-HT did not induced platelet aggregation and release reaction. 5-HT-induced DLT was inhibited by methysergide (0.3～30μmol/L), indomethacin (0.3～10μmol/L) and PGE_1 (0.01～0.3μmol/L) in a dose-dependent manner, but not EGTA(0.3～3mmol/L). Collagen(Coll), arachidonic acid (AA), adenosine diphosphate (ADP) and a stable thromboxane A2 analoge(STA_2) also induced DLT before aggregation by themselves, which was also inhibited by PGE_1, but not inhibited by EGTA except for Coll However,Coll-, AA-, STA_2-and ADP-induced DLT were reduced by pretreatment of PRP with 5-HT dose-depen-dently. The duration of DLT by Coll and AA were decreased from 151.4±5.93 sec and 15(?)38±0.60sec to 45.44±4.04 sec and 9.00±1.25 sec respectively ((?)±s(?) P<0.01), but not by ADP and STA_2, 3μmol/L of ADP-and 0.3μmol/L of STA_2-induced aggregation which was not accompanied with release reaction were enhanced by 5-HT pretreatment, but in those (Coil 5μg/ml, AA 100～200μmol/L and STA_2 1～3 μmol/L) with release reaction, the amount of adenosine triphosphate(ATP)were suppressed significantly (P<0.001) by 5-HT pretreatment without the effect on the magnitude of aggregation, The mobilization of cytosolic free calcium concentration ([Ca~(2+)]i) was observed after 5-HT treatment in single washed platelet, the results indicated that the basic level of [Ca~(2+)] i was 64.78±3.24nmol/L and this level was increased dose-dependently and significantly at 30 sec after administration of 5-HT and the time of peak value of [Ca~(2+)] i was at 90～100 sec.The similar time courses of suppression of ATP released during aggregation, in cases of Coll(5μg/ml), AA (200μmol/L) and STA_2(3μmol/L), by 5-HT were also found in the present experiment.     

细叶小素有效成份药理研究——Ⅳ—3结晶对离休豚鼠心房收缩力的影响

Ⅳ—3结晶系由细叶小叶(Berberis P-oiretii Schn)中提出的一种生物碱。据我室研究证明,Ⅳ—3结晶对小白鼠有明显的耐缺氧和增加心肌对86铷和131铯的摄取作用。本文研究其对离体豚鼠心房收缩力的影响,并初步探讨其对心房影响的作用机理。     

卫矛药理作用的初步研究(Ⅱ)

冠心病心绞痛是常见病、多发病,是危害人民健康的严重疾病之一,因而筛选有效的抗心绞痛药,特别是中草药,是药理工作者的重要任务之一。中草药卫矛又名鬼箭羽(EyonymusSarosancta)是卫矛科落叶灌木,盛产于黑龙江省,来源丰富,四季都可采集。药用其茎及翅状附属物,早在神农本草,新修本草及本草纲目中叙述:“崔氏方疗恶疰在心,痛不可忍,有鬼箭羽汤”;集验方疗卒暴心痛。但多年来未被重视,也很少用于治疗疾病。1977年我校附属第一医院用于治疗心绞痛,有一定疗效。我们也对其药理作用进行了初步研究。本文进一步研究其对血液动力学影响。     

小檗胺对ATP诱导的培养平滑肌及心肌细胞内游离钙动员的影响

目的:研究小檗胺(Ber)对ATP诱导的细胞内钙动员的作用。方法:以Fura 3-AM负载培养的VSMC和心肌细胞,共聚焦技术检测细胞内钙荧光强度的变化。结果:(1)ATP使VSMC和心肌细胞内钙升高,但VSMC核区不明显。(2)Ber对静息荧光强度无影响,但降低ATP升高的胞内钙(P<0.01),Ber 100μmol·L~(-1)延长达峰值的时间(P<0.01),并不能完全抑制ATP升高的胞内钙。(3)在无外钙时,Ber对ATP诱导的胞内钙升高无抑制作用(P>0.05)。(4)Ber的作用与维拉帕米(Ver)相似。结论:ATP引起细胞外钙内流和内钙释放,Ber可阻断ATP引起的外钙内流,对其内钙释放无影响。     

消银片抗炎作用的研究

(1)消银片对小鼠耳廓毛细血管通透性有明显抑制作用。(2)消银片对鸡蛋清引起的炎症有抗炎作用。     

N-甲基小檗胺抗实验性心律失常作用的研究

静脉注射N-甲基小檗胺后,可以明显推迟乌头碱所致大鼠室性早搏出现的时间;明显推迟哇巴因所致豚鼠室早、室颤和停搏出现的时间。该作用呈剂量依赖性。     

自做《医学微生物学》多媒体在教学中的应用

在医学微生物的教学中，利用本教研室现有资源，以及通过图书馆查阅、从医学网站下载相关资料等方式所积累的大量素材，制作一部《医学微生物学》多媒体电子教案，并在实际教学中予以应用，做到理论与临床实践相结合，理论与实验相结合，充分调动了学员的学习积极性，提高了教学效果。     

N-甲基小檗胺对离体豚鼠心房自律性及正性肌力作用的影响

在离体豚鼠左心房,N-甲基小檗胺明显抑制肾上腺素和毒毛旋花子甙K所诱发的异位节律及明显右移毒毛旋花子甙K的正性肌力作用,其作用呈剂量依赖性。     

小檗胺和黑豆果油预防大鼠血脂紊乱作用的研究

本实验证明了无论是单用小檗胺，黑豆果油或两者合用均能预防大鼠血中胆固醇，甘油三酯，低密度脂蛋白含量的升高，复方用药不能增强其降血脂效果；复方大剂量组与小剂量组存在一定的量效关系；单方或复方用药预防血脂升高作用均强于烟酸肌醇酯。     

口服黄谷素药代动力学的研究

口服黄谷素药代动力学的研究药理学教研室李宝新，包丽华，王慧珍，郝晓敏，杨宝峰，李文汉本实验采用反相ＨＰＬＣ方法对健康成人口服黄连素单制剂及口服黄谷素复方制剂的药代动力学进行了研究。方法及结果健康成人１０例，试验前４周停用任何药物，禁烟酒。实验口服黄连...