Parkin基因启动子区-258T/G多态性与广西地区散发性帕金森病的相关性分析

Objective To investigate the association between the-258T/G polymorphism.in the pro-moter of parkin gene and the risk for sporadic parkinson's disease (SPD) in Guangxi Province, and in relationto the age of onset, of PD patients. Methods PCR-RFLP and sequence analysis were used to determine thegenotype of-258T/G polymorphism between all patients and healthy controls. Results The G allele was morecommon in patients than controls (55.20%:43.33% ,x2=6.898, P<0.05, OR=1.61, 95% CI: 1.13 ～2.30). The frequency of GG genotype was higher in patients than in controls (28.00 %: 18.33%, x2=7.159, P<0.05, OR=2.75, 95% CI : 1.31 ～ 5.77). The frequency of TG + GG genotype was higher in pa-tients than in controls (82.40%:68.33%, x2=6.551, P<0.05, OR=2.17.95%CI: 1.20 ～3.93). Afterbeing stratified by onset age, the frequencies of the G allele and GG genotype were significantly higher in pa-tients with onset age over 50 years than those in controls respectively. On the other hand, the frequency was notsignificantly different between the younger onset PD patients and the controls. Conclusion The parkin promot-er-258T/G polymorphism might be a risk factor for PD in Guangxi Province, and the G allele was increasedwith increasing age.     

Parkin基因启动子区-258T/G多态性与广西地区散发性帕金森病的相关性分析

Objective To investigate the association between the-258T/G polymorphism.in the pro-moter of parkin gene and the risk for sporadic parkinson's disease (SPD) in Guangxi Province, and in relationto the age of onset, of PD patients. Methods PCR-RFLP and sequence analysis were used to determine thegenotype of-258T/G polymorphism between all patients and healthy controls. Results The G allele was morecommon in patients than controls (55.20%:43.33% ,x2=6.898, P<0.05, OR=1.61, 95% CI: 1.13 ～2.30). The frequency of GG genotype was higher in patients than in controls (28.00 %: 18.33%, x2=7.159, P<0.05, OR=2.75, 95% CI : 1.31 ～ 5.77). The frequency of TG + GG genotype was higher in pa-tients than in controls (82.40%:68.33%, x2=6.551, P<0.05, OR=2.17.95%CI: 1.20 ～3.93). Afterbeing stratified by onset age, the frequencies of the G allele and GG genotype were significantly higher in pa-tients with onset age over 50 years than those in controls respectively. On the other hand, the frequency was notsignificantly different between the younger onset PD patients and the controls. Conclusion The parkin promot-er-258T/G polymorphism might be a risk factor for PD in Guangxi Province, and the G allele was increasedwith increasing age.     

中风始发态证候与血浆脑肠肽的相关性

目的探讨中风始发态中医证候与血浆脑肠肽CCK(cholecystokinin,CCK)水平的相关性。方法对确诊急性脑出血或急性脑梗死(发病时间<72h)患者采用RIA法检测血浆CCK-8水平,根据中医证候诊断标准对纳入患者进行证候辨证,分析不同证候间血浆CCK-8水平的差异。结果肝阳上亢证患者血浆CCK-8水平较非肝阳上亢证患者显著升高(P<0.01)。结论中风始发态中医证候与血浆CCK水平有相关性。     

SARS与急性上呼吸道感染中医证候分型及指标数量化方法的探讨

目的 探讨早期SARS与急性上呼吸道感染患者中医证候及四诊指标数量化,为中医证候客观化研究提供方法 学参考.方法 应用自编急诊发热病例中医证候观察表,收集急诊SARS患者与急性上呼吸道感染患者中医临床四诊信息,建立数据库,运用样品聚类分析及频数归一化权重分析.结果 早期SARS患者的中医证型主要有两型;急性上呼吸道感染患者早期证候主要有三个证型.结论 样品聚类分析与频数归一化权重方法 可用于SARS患者与急性上呼吸道感染患者的中医证候客观化研究,也可用于其他病的中医证候客观化研究.     

绒毛鼠尾草(林芝丹参)抗肉芽肿形成和改善血液流变学药理试验

目的:比较绒毛鼠尾草(林芝丹参)水提物及CO2萃取物、山东丹参水提物及CO2萃取物抗肉芽肿形成和改善血液流变学作用.方法:抗肉芽肿试验;测定血液流变学指标.结果:林芝丹参和山东丹参均能改善血液流变学,两者之间无显著差异.林芝丹参和山东丹参均无抗肉芽肿作用.结论:林芝丹参对血液流变学的改善作用与山东丹参相似.     

岭南林夏泉流派擅用虫类药治疗癫痫经验

林夏泉老中医认为癫痫病机本质在于肝、脾、肾脏气失和,风、痰、瘀、虚交错为患,自创经验方除痫散,运用虫类药物疏逐搜剔、灵动之性治疗难治性癫痫,临床取得很好疗效,该流派代表性医家刘茂才教授在林老除痫散基础上,制成益脑安胶囊,临床治疗癫痫,收效满意,体现了该流派运用虫类药息风、涤痰、化瘀、定痫的特色。     

中医药联合亚低温脑保护治疗的研究进展

本文通过文献分析探讨中医药联合亚低温脑保护治疗的疗效及应用前景。回顾亚低温脑保护治疗的作用机制及临床研究现状,并对近十年来国内有关中医药联合亚低温脑保护治疗的实验研究及临床研究文献作出分析。动物实验表明,适当的中医药联合亚低温脑保护治疗能更好地起到脑神经保护作用;但临床研究文献报道并不多见,需要经过大量、科学的研究和评估以进一步证实中医药联合亚低温脑保护治疗的临床疗效。中医药联合亚低温脑保护治疗的应用,目前属于探索阶段,为临床治疗脑血管病提供了一个新的思路,两者的联合应用具有广阔的前景。     

短期胰岛素泵强化治疗对初发2型糖尿病血管内皮功能影响的临床观察

目的：利用彩超测定肱动脉血流介导的血管舒张功能(FMD)评价2型糖尿病患者经2 w胰岛素泵强化治疗后,血管内皮依赖性舒张功能改善情况。方法对34例初诊2型糖尿病患者进行2 w的胰岛素泵强化治疗,分析比较其治疗前后血糖、Homa-IR、血脂及FMD水平。结果胰岛素泵强化治疗后FPG、2 hPG、HbA1c、血脂水平下降,Homa-IR降低,FMD水平明显升高。结论初诊2型糖尿病患者,短期胰岛素泵强化治疗能迅速平稳控制血糖、降脂、减轻胰岛素抵抗,并能显著改善FMD。     

李振华教授辨治瘀血头痛经验

瘀血头痛是头痛常见的临床类型,其范围有二:①为跌扑损伤,头部外伤,头面部瘀血阻滞脑窍;②各种内伤头痛反复发作,久病入络,久病多瘀,久病及肾,脑络为瘀血凝滞。辨治瘀血头痛,要善于运用辛温通络、活血通窍法,后期以滋补肝肾,健脑生髓为主。总结名老中医李振华教授经验,在通窍活血汤基础上加强辛温通络之品及虫类药组成“通窍止痛汤”,并用活血养血、滋补肝肾、健脑生髓的“补脑汤”治疗瘀血头痛,疗效显著。     

199例头风病患者证候特点及与血小板5-羟色胺关系研究

目的：对头风病患者证候分布、临床特点及与血小板5-羟色胺关系进行分析。方法：199例头风病患者分为风火候、风痰候、风瘀候、正虚候,观察各证候间年龄、头痛病程、头痛指数差异及与血小板5-羟色胺关系,及头风病各症候与风、火、痰、瘀、郁、气虚、血虚各致病因素之间的相关性。结果：各证候间病程、头痛程度、持续时间比较,差异有非常显著性意义（P〈0.01）,各证候间血小板5-羟色胺浓度比较,差异也有显著性意义（P〈0.05）。风火候易于与致病因素郁相夹杂致病（P〈0.01）;风痰候易于与致病因素瘀和气虚相夹杂致病（P〈0.01）;正虚候易于与致病因素痰相夹杂致病（P〈0.05）;风瘀候易于与致病因素痰和血虚相夹杂致病（P〈0.05）。结论：风火候是头风病最常见的证候类型,头风病各证候间血小板5-羟色胺浓度有显著差异。