血管紧张素Ⅱ上调BaX表达诱导人内皮细胞凋亡

为了研究血管紧张素Ⅱ(AngⅡ)诱导凋亡的机制,将人脐静脉内皮细胞培养至第3代,用不同浓度Ang Ⅱ培养不同时间,用TUNEL检测细胞凋亡.Bax和Bcl-2蛋白表达用Western Blot和图像分析法测定.结果发现Ang Ⅱ组的凋亡阳性率极显著高于对照组;Bax的表达极显著地升高且具有时间和剂量依赖性,Bcl-2无显著变化,Bax/Bcl-2比值极显著地增加.提示AngⅡ通过诱导Bax的高表达,致Bax/Bcl-2比值极显著地增加而诱发凋亡.     

重庆康复医学工程研究会首次举办康复医疗学习班

重庆康复医学工程研究会举办的重庆市首届康复医疗学习班于5月21日结束。参加这次学习班学习的学员来自省内外10个市县。学习班总计授课99学时,分7章31节。主要目的在于使学员对新兴的康复医学有所了解,培养初具康复医学知识的人才,为各地开创康复医疗事业打下基础。     

p^21／waf1调节血管紧张素Ⅱ的抗增生作用

为了研究血管紧张素 (Ang )的抗内皮细胞增生作用及其分子生物学调节机制 ,将健康胎儿脐静脉内皮细胞培养至第 3代 ,用 Ang 培养并在不同时间计数细胞密度。用 TUNEL 或 EL ISA方法证明 Ang 或 CGP42 112 A诱导内皮细胞凋亡。用 RT- PCR和 Western Blot检测 p2 1 / waf1m RNA和 P2 1 蛋白的表达并测定条带的光密度。结果表明 ,在 Ang 培养后不同时间 ,细胞密度比对照减少 30 %左右 (P<0 .0 1)。Ang 作用后可以诱导典型的凋亡 ,阳性率极显著高于阴性对照组并具有剂量依赖性。Ang 组或 CGP42 112 A组的 p2 1 / waf1m RNA表达分别是对照组的 (4 73.6 9± 39.97) %和 (391.5 8± 48.2 4) %(P<0 .0 1)。Ang 组的 P2 1 蛋白表达比对照组极显著地增加并具有时间依赖性。认为 Ang 具有抗内皮细胞增生作用 ,其细胞内机制是同时诱导凋亡和 p2 1 / waf1m RNA与 P2 1 蛋白的高表达 ,使细胞发生 G1 期阻滞 ,增殖受抑。     

TRENCH FEVER A RESUME OF LITERATURE AND A NOTE ON SOME OBSCUP\E PHASES OF THE DISEASE

Although trench tever is now known to be almost worldwide in distribution, it usually exists unrecognized in louse-infested cornm.uni- ties in peace time, and the disease may flare up in epiclemic form in wartime. Inasmuch as phy- sicians are generally unfamiliar with trench fever, the literature on the history, geographic distribution, symptomatology, pathology, etio- logy, immunity, diagnosis, epidemiology, pre- vention and treatment of the diseas.e iS briefly reviewed. Certain obscure phases of trench fe- ver are noted and lines for further studies of the disease suggested. The question is enterta.in- ed whether R rocha-lima, a rickettsia of hitherto undefined status., may represent a variant of R prowazeki acquired by lice feeding on typhus oonvalescent,s living under nat.urall conditions and an intermedia.te stage in the transformation of R prowazeki in the direction of R quintana.     

AN ATTEMPT TO INFECT HUMAN BODY LICE, PEDICULUS HUMANUS VAR CORPORIS ON A MILD CASE OF MURINE TYPHUS*

A patient with a sporadic mild case of ty- phus fever assume on epidemiological and cli- nical grounds to be murine typhus was test.ed for his infectivity for normal adult human body lice, Pediculusi humanus va.r corporis, during a period of 5 days from the 9th through 4he 13;th or last day of the disea.se. One out of 105 mice (0.95'/o) was later found to have developed a small numbeir o,f morphologically and tincto- rially typical ricketts.iae. A guinea pig, which was inoculated in succes.sion with two suspen- sions of ground lice showing and n;olt showing definite rickettsia.e in smears, twice gave ne- gative complement fixation with epidemic anti- gen but positive fixation with murine antigen at a titer of l:40. As far as the authors. are awa.re, this is the first report to show that by feeding on a mild case of murine typhus, lic;e became infected with typhus rickettsiae which proved serologically to be R. mooSieri. It is therefore suggested that under nat.ural condi- tions when lice feed continuously on severe cases of murine typhus throughouit the febrile disease, a sufficiently higher percent of lice may become infected with sufficiently larger numbers of typhus ricketts.iae to give rise to outbreaks of louse-borne typhus in Iouse-infested communi- ties.     

血管紧张素Ⅱ诱导人内皮细胞凋亡

为探讨血管紧张素Ⅱ(AngⅡ)能否诱导人内皮细胞凋亡,将健康胎儿脐静脉内皮细胞培养至第3代,用不同浓度AngⅡ培养不同时间,用TUNEL和ELISA检测细胞凋亡,并检验Caspase-3酶活性变化.用RT-PCR检测AngⅡ受体AT1和AT2的mRNA表达.发现AngⅡ可以诱导出典型的凋亡,凋亡细胞阳性率极显著高于对照组,凋亡强度与AngⅡ呈现剂量依赖关系.Caspase-3酶活性在AngⅡ作用后有极显著的增加.内皮细胞同时表达AT1和AT2的mR-NA.结果表明AngⅡ经AT1或(和)AT2可以诱导人内皮细胞凋亡,并且是Caspase-3依赖性的.这一结果有助于阐明AngⅡ的致病机制.     

AT2受体介导入内皮细胞凋亡

研究了AT2受体在血管紧张素Ⅱ（AngⅡ)及2型血管紧张素Ⅱ受体（AT2）兴奋剂CGP42112A作用下能否诱导人内皮细胞凋亡及其与半胱氨酰蛋白酶（cystolic aspartatic proteases)之Caspase-3的关系。结果显示，AngⅡ或CGP42112A可以诱导人内皮细胞出现典型的细胞凋亡，凋亡细胞阳性率极显著高于阴性对照组，凋亡的相对强度与AngⅡ呈现典型的剂量依赖关系，Cas-pase-3酶活性在AngⅡ或CGP42112A作用后有极显著的增加，而在用Caspase-3抑制剂DEVD-fmk后凋亡不能诱导，提示AngⅡ或CGP42112A可以通过AT2受体诱导人内皮细胞凋亡，并且此凋亡是Caspase-3依赖性的。     

血管紧张素Ⅱ诱导人内皮细胞凋亡

为探讨血管紧张素Ⅱ（Ang Ⅱ）能否诱导人内皮细胞凋亡,将健康胎儿脐静脉内皮细胞培养至第3代,用不同浓度Ang Ⅱ培养不同时间,用TUNEL和ELISA检测细胞凋亡,并检验Caspase-3酶性变化,用RT－PCR检测Ang Ⅱ受体AT1和AT2的mRNA表达。发现Ang Ⅱ可以诱导出典型的凋亡,凋亡细胞阳性率极显著高于对照组,凋亡强度与Ang Ⅱ呈现剂量依赖关系。Caspase-3酶活性在Ang Ⅱ作用后有极显著的增加。内皮细胞同时表达AT1和AT2的mRNA。结果表明：Ang Ⅱ经AT1或（和）AT2可以诱导人内皮细胞凋亡,并且是Caspase-3依赖性的。这一结果有助于阐明Ang Ⅱ的致病机制。     

重庆市康复中心江北康复医院简介

重庆市康复中心江北康复医院,座落在地处长江嘉陵江汇合口的江北城茅家山。医院环境幽美,依山傍水,空气清新,交通方便。远眺可饱览美丽山城的天然风姿,近看满目青翠令人心旷神怡,是康复的理想地方。该中心成立于一九八四年五月八日。两年来在党和政府的亲切关怀和支持下,在重庆康复医学工程研     

肺血流图与右心功能相关性研究

本文对比研究了慢性阻塞性肺病（慢阻肺）肺心病患者的肺血流图和右心功能，建立了计算右心功能的回归公式，为临床应用提供依据。1资料和方法患者34例，男32例，女2例。平均年龄59.50±10.40岁，按1980年第3次全国肺心病专业会议制定的标准，排除其它疾患，均诊断为慢阻肺或并有肺心病（无明显心衰）。有9例患者在平均15天后复查了右心功能和肺血流图。右心功能检查，按文献[1]描述的Fick法测量计算右心功能。4F微导管，TP-601型压力传感器。RM-6000型八道生理仪。ABL-Ⅲ型血气分析仪，OSP-Ⅲ型氧耗仪。计算出每分心输出量（CO，L／m…