低剂量辐射增强小鼠肿瘤基因-放射治疗效应的免疫学机制研究

目的 探讨低剂量辐射全身照射对小鼠Lewis肺癌移植肿瘤基因-放疗方案中的免疫增强作用机制。方法 小鼠右后肢皮下接种Lewis肺癌细胞建立荷瘤模型,基因-放疗组中小鼠肿瘤局部注射由多聚乙烯亚胺包裹的pEgr-IL18-B7.1重组质粒,分别接受由2 Gy 局部照射和0.075 Gy 全身照射组合的不同治疗方案,通过³H-TdR标记方法检测小鼠CTL和NK细胞毒活性,ELISA方法检测TNF-α和IFN-γ分泌活性,观察各治疗组对荷瘤小鼠抗肿瘤免疫的作用。结果 在pEgr-IL18-B7.1基因治疗方案中,单次大剂量辐射局部照射后加多次低剂量全身照射与常规多次大剂量辐射局部照射相比,小鼠CTL和NK细胞毒活性显著增强,TNF-α和IFN-γ分泌活性有不同程度的增高。 结论 低剂量辐射可以通过促进CTL和NK细胞毒效应,上调TNF-α和IFN-γ细胞因子表达,从而增强机体抗肿瘤免疫功能,提高肿瘤基因-放疗的抑瘤效果。     

Schizophrenia susceptibility genes on chromosome 13q32

Schizophrenia is a complex mental disorder affecting approximately 1% of the general population worldwide.1 It has a high incidence in the general population, a poor prognosis and a poor outcome, in that it has become a major social problem. Family, twin, and adoption studies have clearly shown that a genetic component is quite likely to play an important role in determining susceptibility to schizophrenia. The genome-wide scan indicates that several chromosomal regions are linked to schizophrenia, some of which have been replicated independently including 6p21-24, 8p21-22, 13q14-33 and 22q11-12.2,3 This study was designed to detect two single nucleotide polymorphisms (SNPs) located in the 13q14-33 region, rs188608 at the STK24 locus and rs2892679 at the GPC6 locus, among Chinese population.     

低剂量辐射的抑瘤作用及其机理的研究现状

低剂量辐射的抑瘤作用及其机理的研究现状傅海青，李修义，刘树铮１９６５年Ｈｏｌｄｅｒ［１］等就报道了低剂量全身照射对多发性骨髓瘤患者的临床效果，１９６７年Ｊｏｎｓｏｌ［２］等首次将低剂量全身照射用于治疗非何杰金氏淋巴瘤，并肯定了其抗肿瘤效果，Ｃｈａｆｆ...     

照射与免疫的时间间隔对抗体形成的影响及其辐射剂量效应

本实验观察了0. 25、0. 5、1. 0、2. 0、3. 0和4. 0Gy全身照射后3至6 h羊红细胞(SRBC)免疫对小鼠脾脏抗体形成细胞(PFC)的影响。结果发现,各剂量照射后,免疫与照射的时间间隔较长者,辐射对PFC反应的抑制显著加深。照射后3及6h免疫,脾脏PFC反应均随照射剂量的增加而降低。照射后3h免疫时,PFC反应的D_(37)值为4. 13Gy;照射后6h免疫时,PFC反应的D_(37)值为1. 74Gy。     

Low dose radiation effects.

The International Symposium on Biological Effects of Low Level Exposures to Radiation and Related Agents (ISBELLES'93), sponsored by the Chinese Society of Radiological Medicine and Protection, Chinese Medical Association, was held in Changchun, China on 12-16 October, 1993, and was attended by 112 scientists from 11 countries and areas. The aim of the symposium is to bring together and assess current scientific information and provide for interchange between researchers in related areas. The Organizing Committee had received 121 papers from 14 countries and districts before the opening of the symposium. Twelve overview lectures and 55 proffered papers were presented at the symposium in different subject areas of biological effects of low level exposures to radiation and other environmental agents, including human observations, animal models, cellular and subcellular effects, molecular mechanisms and systemic regulation.     

头部X线照射后大鼠血清中皮质酮含量和脾细胞中糖皮质激素受体变化及其相互关系的研究

给Wistar大鼠以10Gy X射线头部照射后不同时间测定血清皮质酮含量和脾细胞中糖皮质激素受体,结果表明:头照后24h受体数已降到对照组的25％,以后逐渐回升,至第9天恢复到接近正常水平。头照后受体的最大结合容量有降低趋势,但差异不显著,而Kd值显著增大,亲和力降低。皮质酮含量的变化呈双峰曲线,第1天即显著增高,至第9天仍显著增高,皮质酮含量和受体的变化规律不一致。体外实验还证实了1～10倍血清浓度的皮质酮在短时间内不会引起所测定的GCR改变。证明皮质酮的变化虽然可能是引起受体变化的一个原因,但不是主要原因。     

抑制素(Inhibin)的研究 Ⅰ.人精液抑制素的制备及其生物学特性

本文报道人精液抑制素的制备及其生物学特性。收集的新鲜人精液经乙醇沉淀和丙酮冲洗,获得的粗提物经Sephadex G100处理,产生具有高活性抑制素hP2组分。应用hP2测定使去势雄性大鼠增高的血清FSH水平降低。其生物特性:hP2的生物活性按单位重量计算为粗提物的10倍以上,包含16种氨基酸,通过SDS-PAGE计算分子量为11,500道尔顿。     

低剂量电离辐射对小鼠免疫器官Bcl-2蛋白表达的影响

目的 :研究低剂量电离辐射对小鼠胸腺、脾和肠系膜淋巴结 Bcl- 2蛋白表达的影响 ,以揭示辐射诱导细胞凋亡 J型曲线的分子机理。方法 :采用免疫组织化学及图像分析定量方法。结果 :假照组各免疫器官存在 Bcl- 2蛋白的基础表达 ;而照射组随着照射后时间的推移 ,Bcl- 2蛋白表达逐渐增高 ,2 4 h达峰值 ,且差异显著 ;72 h回复至假照水平。结论 :上述结果为揭示低剂量辐射免疫兴奋效应和辐射诱导细胞凋亡 J型曲线的分子机理提供了有意义的数据