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目的 研究预防性运用不同剂量甲基强的松龙(MP)对脊髓缺血再灌注损伤(SCII)后热休克蛋白27(Hsp27)表达的不同影响,探讨运用MP预防SCII的最佳剂量.方法 随机将100只大鼠平均分成A、 B、 C、 D、 E五组,采用夹闭腹主动脉后再灌注的方法建立大鼠SCII模型.A组不夹闭腹主动脉,余组用上述方法制SCII模型,C、D、E三组在术前30 min分别预防性应用10、20、30 mg/kg MP,B组给予同等剂量生理盐水,都通过尾静脉注射给药.3 h后取各组大白鼠脊髓标本,行HE染色观察,并用免疫组化的方法测量Hsp27的相对含量.结果 E组大鼠神经元的形态改善最明显.E组Hsp27的表达量最高,五组之间总体上差异有统计学意义(P<0.05).结论 预防性运用30 mg/kg MP可使对脊髓缺血再灌注损伤具有保护作用的Hsp27表达上调最明显,减少脊髓缺血再灌注损伤效果最好. 相似文献
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Objective To observe the effect of methylprednisolone (MP) pre-intervention on ex-pressions of heat shock protein 27 (HSP27) and tumor necrosis factor alpha (TNF-α) in cells in rat spinal cord following ischemia-reperfusion injury. Methods One hundred and fifty male Sprague-Dawley (SD) rats were randomly divided into 3 equal groups: group A (control) in which the abdominal aorta was exposed without any treatment, group B in which the abdominal aorta was clipped for 30 minutes before reperfusion for 3 bours to establish a model of ischemia- reperfusion injury, and group C in which intravenous MP injection was conducted 30 minutes before the establishment of the ischemia-reperfusion injury model. Three hours later the spinal cords were harvested. Pathological changes of spinal cord cells were observed with HE staining and expressions of HSP27 and TNF-α in spinal cord cells were observed with immunohistochemical staining. The motor function of hind-limbs before was evaluated before sample harvest. The data were analyzed with SPSS software. Results There were significant differences between groups A and B in the expressions of TNF-α and HSP27. Compared with group B, the expression of TNF-α decreased and HSP27 increased in group C, with statistically significant differences between the 2 groups. The motor function score of hind-limbs decreased in group B but improved in group C. Conclusions Since MP can decrease the expression of TNF-α and up-regulate the expression of HSP27, it has a potency of neuro-protection. Spinal cord ischemia-reperfusion injury can be avoided or decreased after MP pre-intervention. 相似文献
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目的研究大鼠脊髓缺血再灌注损伤(SCⅡ)后肿瘤坏死因子-α(TNF—d)的表达及甲基强的松龙(MP)预干预对TNF-α【表达的影响。方法健康雄性SD大鼠150只随机分成3组:对照组(n=50)仅手术暴露腹主动脉,不做进一步处理;脊髓缺血再灌注损伤纽(SCⅡ组)(n=50)建立SCⅡ模型,夹闭腹主动脉30min后松开,再灌注3h;甲基强的松龙组(MP组)(n=50)于建立SCⅡ模型前30min尾部静脉注射MP(30mg/kg),而A组和B组于术前30min分别注入等量的生理盐水。3组均取腰段脊髓标本,观察损伤节段脊髓病理形态学变化,用免疫组织化学染色方法观察脊髓组织TNF-α的表达,并对大鼠后肢运动功能进行评分。采用SPSS10.0软件对数据进行统计学处理。结果①SCⅡ组脊髓组织内TNF—d呈高表达,与对照组相比差异有统计学意义(P〈0.05)。②MP组脊髓细胞内TNF-α表达明显降低,与SCⅡ组对比差异有统计学意义(P〈0.05)。③大鼠脊髓缺血再灌注损伤后后肢功能评分降低,MP组后肢运动功能评分提高。结论①SCⅡ后脊髓细胞TNF-α呈高表达。②预防应用MP可降低TNF-α的表达,也即预防应用MP能减轻脊髓缺血再灌注损伤,保护脊髓神经功能。 相似文献
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目的 评价后路经椎弓根半椎体切除、植骨内固定治疗半椎体畸形的疗效.方法 回顾性分析2007年6月至2009年6月共30例接受经椎弓根半椎体切除、植骨内固定治疗的先天性半椎体畸形病例,比较术前、术后脊柱全长正侧位片及脊柱侧凸Cobb角.结果 术前冠状面主弯Cobb角平均46.52°,术后Cobb角14.64°,侧凸平均矫正率68.51%,术前术后主弯Cobb角比较差异有统计学意义(P<0.05);无内固定断裂、出现神经症状病例.结论 后路经椎弓根半椎体切除、融合内固定治疗半椎体畸形可有效地矫正畸形. 相似文献
5.
Objective To observe the effect of methylprednisolone (MP) pre-intervention on ex-pressions of heat shock protein 27 (HSP27) and tumor necrosis factor alpha (TNF-α) in cells in rat spinal cord following ischemia-reperfusion injury. Methods One hundred and fifty male Sprague-Dawley (SD) rats were randomly divided into 3 equal groups: group A (control) in which the abdominal aorta was exposed without any treatment, group B in which the abdominal aorta was clipped for 30 minutes before reperfusion for 3 bours to establish a model of ischemia- reperfusion injury, and group C in which intravenous MP injection was conducted 30 minutes before the establishment of the ischemia-reperfusion injury model. Three hours later the spinal cords were harvested. Pathological changes of spinal cord cells were observed with HE staining and expressions of HSP27 and TNF-α in spinal cord cells were observed with immunohistochemical staining. The motor function of hind-limbs before was evaluated before sample harvest. The data were analyzed with SPSS software. Results There were significant differences between groups A and B in the expressions of TNF-α and HSP27. Compared with group B, the expression of TNF-α decreased and HSP27 increased in group C, with statistically significant differences between the 2 groups. The motor function score of hind-limbs decreased in group B but improved in group C. Conclusions Since MP can decrease the expression of TNF-α and up-regulate the expression of HSP27, it has a potency of neuro-protection. Spinal cord ischemia-reperfusion injury can be avoided or decreased after MP pre-intervention. 相似文献
6.
Objective To observe the effect of methylprednisolone (MP) pre-intervention on ex-pressions of heat shock protein 27 (HSP27) and tumor necrosis factor alpha (TNF-α) in cells in rat spinal cord following ischemia-reperfusion injury. Methods One hundred and fifty male Sprague-Dawley (SD) rats were randomly divided into 3 equal groups: group A (control) in which the abdominal aorta was exposed without any treatment, group B in which the abdominal aorta was clipped for 30 minutes before reperfusion for 3 bours to establish a model of ischemia- reperfusion injury, and group C in which intravenous MP injection was conducted 30 minutes before the establishment of the ischemia-reperfusion injury model. Three hours later the spinal cords were harvested. Pathological changes of spinal cord cells were observed with HE staining and expressions of HSP27 and TNF-α in spinal cord cells were observed with immunohistochemical staining. The motor function of hind-limbs before was evaluated before sample harvest. The data were analyzed with SPSS software. Results There were significant differences between groups A and B in the expressions of TNF-α and HSP27. Compared with group B, the expression of TNF-α decreased and HSP27 increased in group C, with statistically significant differences between the 2 groups. The motor function score of hind-limbs decreased in group B but improved in group C. Conclusions Since MP can decrease the expression of TNF-α and up-regulate the expression of HSP27, it has a potency of neuro-protection. Spinal cord ischemia-reperfusion injury can be avoided or decreased after MP pre-intervention. 相似文献
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目的 探讨Pilon骨折的治疗方法和影响疗效的因素.方法 对36 例不同类型(Ruedi-Allgow er 分型) 的Pilon骨折患者选用保守治疗、有限内固定、坚强内固定、有限内固定结合外固定架治疗,并分析其疗效.结果 随访6个月~2 年,参照Helfet 提出的标准判断,总优良率为80.1%,胫距关节面达到解剖复位的骨折的疗效优良率达89.6%.结论 临床疗效与骨折类型、治疗方法有关,早期解剖复位和坚强内固定是治疗的关键. 相似文献
9.
Objective To observe the effect of methylprednisolone (MP) pre-intervention on ex-pressions of heat shock protein 27 (HSP27) and tumor necrosis factor alpha (TNF-α) in cells in rat spinal cord following ischemia-reperfusion injury. Methods One hundred and fifty male Sprague-Dawley (SD) rats were randomly divided into 3 equal groups: group A (control) in which the abdominal aorta was exposed without any treatment, group B in which the abdominal aorta was clipped for 30 minutes before reperfusion for 3 bours to establish a model of ischemia- reperfusion injury, and group C in which intravenous MP injection was conducted 30 minutes before the establishment of the ischemia-reperfusion injury model. Three hours later the spinal cords were harvested. Pathological changes of spinal cord cells were observed with HE staining and expressions of HSP27 and TNF-α in spinal cord cells were observed with immunohistochemical staining. The motor function of hind-limbs before was evaluated before sample harvest. The data were analyzed with SPSS software. Results There were significant differences between groups A and B in the expressions of TNF-α and HSP27. Compared with group B, the expression of TNF-α decreased and HSP27 increased in group C, with statistically significant differences between the 2 groups. The motor function score of hind-limbs decreased in group B but improved in group C. Conclusions Since MP can decrease the expression of TNF-α and up-regulate the expression of HSP27, it has a potency of neuro-protection. Spinal cord ischemia-reperfusion injury can be avoided or decreased after MP pre-intervention. 相似文献
10.
Objective To observe the effect of methylprednisolone (MP) pre-intervention on ex-pressions of heat shock protein 27 (HSP27) and tumor necrosis factor alpha (TNF-α) in cells in rat spinal cord following ischemia-reperfusion injury. Methods One hundred and fifty male Sprague-Dawley (SD) rats were randomly divided into 3 equal groups: group A (control) in which the abdominal aorta was exposed without any treatment, group B in which the abdominal aorta was clipped for 30 minutes before reperfusion for 3 bours to establish a model of ischemia- reperfusion injury, and group C in which intravenous MP injection was conducted 30 minutes before the establishment of the ischemia-reperfusion injury model. Three hours later the spinal cords were harvested. Pathological changes of spinal cord cells were observed with HE staining and expressions of HSP27 and TNF-α in spinal cord cells were observed with immunohistochemical staining. The motor function of hind-limbs before was evaluated before sample harvest. The data were analyzed with SPSS software. Results There were significant differences between groups A and B in the expressions of TNF-α and HSP27. Compared with group B, the expression of TNF-α decreased and HSP27 increased in group C, with statistically significant differences between the 2 groups. The motor function score of hind-limbs decreased in group B but improved in group C. Conclusions Since MP can decrease the expression of TNF-α and up-regulate the expression of HSP27, it has a potency of neuro-protection. Spinal cord ischemia-reperfusion injury can be avoided or decreased after MP pre-intervention. 相似文献