剖宫产产妇手术室心理特点及护理

剖宫产非正常分娩,产妇会产生恐惧、焦虑、自信心降低、孤独等心理,这些不良心理因素不利于手术的进行。为了消除这些不良心理,手术室护士不但注重对产妇的一般护理,更应注重产妇的心理护理,通过行为、语言、态度、形体等各个方面,改变产妇不良的心理状态,提高产妇接受手术的心理承受能力,使产妇处于一个健康的接受手术的最佳状态,确保手术顺利进行。     

经络动物模型初探

人体经络循经现象显示经络与植物神经末梢结构和功能关系密切。通过比较解剖学等方面的探讨,我们初步明确经络可能是原始子午干神经系结构和功能在人体中的某种保留,故此对有关动物神经系的结构和功能进行了初步观察,探讨与经络的联系,有了一点粗浅的体会。现简介如下。     

应用干扰素治疗慢性乙型肝炎的心理护理

目的:探讨干扰素治疗慢性乙型肝炎的心理护理。方法:我科2011年1月至2011年12月应用干扰素治疗慢性乙型肝炎的心理护理及不良反应护理方法。结论:护士在干扰素治疗过程中有针对性地进行心理护理,提高依从性,保证治疗效果。     

CEPO对6-OHDA诱导PC12细胞损伤的保护作用

目的探讨氨甲酰化促红细胞生成素(CEPO)对6-羟基多巴胺(6-hydroxydopamine,6-OHDA)诱导PC12细胞损伤及凋亡的保护作用及其可能机制。方法借助CCK8、流式细胞(Flow cytometry,FCM)、Western-blotting和逆转录PCR(RT-PCR)技术检测PC12模型细胞相关指标的变化,实验数据以SPSS15软件统计分析。结果 CCK8结果显示6-O-HDA处理能够显著降低PC12模型细胞的存活率,而CEPO处理对其变化显示抑制作用;FCM技术探察结果显示,6-OHDA处理能够明显诱导PC12模型细胞的损伤与凋亡,而CEPO预处理能够减轻PC12模型细胞的损伤与凋亡(P<0.05);借助Western-blotting技术探察显示6-OHDA+CEPO处理组的PC12细胞的Cleaved caspase-3蛋白表达量显著低于6-OHDA处理组(P<0.05);RT-PCR结果显示,6-OHDA处理明显降低PC12模型细胞的Bcl-2 mRNA的表达(P<0.05)以及上调PC12模型细胞的Bax mRNA的表达(P<0.01),而CEPO处理明显上调Bcl-2 mRNA的表达水平(P<0.05)以及抑制Bax mRNA的表达(P<0.05)。结论CEPO处理能够显著抑制6-OHDA所诱导的PC12细胞的损伤及凋亡,其作用机制可能与其上调Bcl-2,并下调Bax和Caspase-3的表达有关。     

易善复药液与其他注射液存在配伍禁忌

易善复注射液又名肝得健,主要成分为容易吸收利用的高能多烯磷脂酰胆碱,具有使受损的肝功能和酶活力恢复正常,调节肝脏能量平衡,促进肝组织再生等生理功能,临床多用来     

利用神经通路示踪方法证实大鼠纹状体神经元的连接

Using neural pathway tracing and immunohistochemical technique, the striato-direct pathway (BDA3 kDa injected into the rat lateral globus pallidus) and striato-indirect pathway (BDA3 kDa injected into the substantia nigra pars reticulata) neurons were specifically labeled, and then subjected to double-labeled immunohistochemistry for mu-OPIOID Receptor (specifically-labeled striatal patch compartment), D1, and D2, respectively. The experimental findings showed that there are no statistically significant differences in the soma diameter and the number of primary dendrites between the striato-direct (substantia nigra pars reticularis) and indirect (globus pallidum externum) neurons labeled retrograde by BDA3 kDa. In addition, these two kinds of projection neurons revealed no obvious coexistence. This evidence indicates that as a highly sensitive neural pathway tracer, BDA could yield reliably and exquisitely detailed labeling of target neurons and synaptic structures. The variance of the morphologic structures and the localization of neurons were not statistically significant between the striato-substantia nigra pars reticularis and the globus pallidum externum projection neurons. Mesencephalic and thalamic neurons correlated with striatal neurons in morphology. Especially the latter which make typical excitatory synaptic contacts with striato-direct and -indirect neurons. Thus, this evidence suggests that thalamic neurons may extensively excite striatal neurons.     

肝穿刺活检术预见性护理42例效果分析

目的总结分析肝穿刺活检术预见性护理的临床效果。方法回顾我院2011年1月～2011年10月42例行肝穿刺活检术患者的临床资料,分析实施预见性护理对肝穿刺活检术的影响。结果本组患者采用预见性护理,对肝穿刺活检术前、术中及术后可能出现的并发症、不良反应等尽早做出护理干预,减轻了病人的心理负担,所有患者均接受了肝穿刺活检,提高了穿刺成功率,减少了并发症。结论做好肝穿刺活检术的预见性护理,能够提高患者对肝穿刺活检术的认知,减轻患者术前紧张、恐惧心理,从而提高穿刺术成功率,减少并发症的发生。     

大鼠脊髓钙调蛋白Parvalbumin和Calbindin-28KD阳性神经元的形态与分布特征

目的 观察证实钙调蛋白Parvalbumin( PV)和Calbindin-28KD (CB)阳性神经元在大鼠脊髓不同节段不同区域内的形态及分布特征.方法 成年雄性SD大鼠常规灌注固定,取第5～8颈髓、3～6胸髓和1～5腰髓节段;振动切片之后进行免疫组化PAP单标记;光镜观察阳性神经元的形态和分布特征、并进行计数和测量;用Excel软件对阳性神经元数量、胞体大小、突起数量进行统计处理.结果 PV阳性神经元主要存在于后角的Ⅱ层和胸核、中间带外侧、中间内侧核、前角的Ⅷ层;CB阳性神经元主要存在于后角Ⅰ～Ⅱ层、中间带,前角的Ⅷ～Ⅸ层之间.后角细胞较中间带及前角细胞数量多,胞体小,突起少.在不同区域,两种阳性神经元的细胞数量,胞体大小,突起数目都存在统计学差异(P＜0.05);而在不同节段同一区域的比较中,只有腰前角PV阳性神经元的胞体比较大(P＜0.05).结论 PV阳性神经元在胸核及中间内侧核的聚集以及阳性纤维在薄楔束和后角Ⅱ层胶状质内的密集分布提示其与机体痛觉传入、本体感觉及内脏感觉的联系;CB阳性神经元在前角Ⅷ～Ⅸ层之间的特征性分布说明其与闰绍细胞功能有关;两种钙调蛋白在脊髓不同节段之间的形态及分布基本无差别提示它们的功能并没有特异地对应于躯体或内脏.     

心理授权在变革型领导与护士组织公民行为间的中介作用

目的:调查成都市护士长变革型领导能力、护士组织公民行为和心理授权的现状及其三者之间的关系。方法:2023年4月—6月采用便利抽样法选取成都市5所不同等级医院的380名临床护士为研究对象,应用一般资料调查问卷、变革型领导量表(TLQ)、护士组织公民行为量表(OCBS)和心理授权量表(PES)进行调查。结果:护士长变革型领导、护士组织公民行为、心理授权总分分别为(101.76±16.97)分、(99.63±11.04)分、(43.52±6.56)分;Pearson相关性分析结果显示,护士OCBS与TLQ、PES呈正相关(P<0.01),TLQ与PES呈正相关(P<0.01);中介效应分析显示,心理授权在变革型领导与组织公民行为之间起部分中介作用。结论:护理管理者采用变革型领导方式有利于增强护士心理授权程度,从而提高组织公民行为,提示管理者应对护士心理授权进行积极引导。     

Carbon monoxide inhibits the nuclear-cytoplasmic translocation of HMGB1 in an <Emphasis Type="Italic">in vitro</Emphasis> oxidative stress injury model of mouse renal tubular epithelial cells

Carbon monoxide (CO), as a vital small molecule in signaling pathways, is found to be involved in ischemia-reperfusion injury (IRI) in renal transplantation. CO-releasing molecule-2 (CORM-2), a CO-releasing molecule, is a type of metal carbonyl complexes which can quickly release CO in vivo. In this study, an in vitro oxidative stress injury model was established to examine the effect of CORM-2 pretreatment on the nuclear-cytoplasmic translocation of high mobility group box 1 protein (HMGB1) in mouse primary renal proximal tubular epithelial cells (RPTECs). Immunofluorescence staining showed that HMGB1 in the medium- and CORM-2-treated groups was predominantly localized in the nucleus of the cells, whereas higher amounts of HMGB1 translocated to the cytoplasm in the H₂O₂- and inactive CORM-2 (iCORM-2)-treated groups. Western blotting of HMGB1 showed that the total amounts of cytoplasmic HMGB1 in the H₂O₂-treated (0.59±0.27) and iCORM-2-treated (0.57±0.22) groups were markedly higher than those in the medium-treated (0.19±0.05) and CORM-2-treated (0.21±0.10) groups (P<0.05). Co-immunoprecipitation showed that the levels of acetylated HMGB1 in the H₂O₂-treated (642.98±57.25) and iCORM-2-treated (342.11±131.25) groups were markedly increased as compared with the medium-treated (78.72±74.17) and CORM-2-treated (71.42±53.35) groups (P<0.05), and no significant difference was observed between the medium-treated and CORM-2-treated groups (P>0.05). In conclusion, our study demonstrated that in the in vitro oxidative stress injury model of primary RPTECs, CORM-2 can significantly inhibit the nuclear-cytoplasmic translocation of HMGB1, which is probably associated with the prevention of HMGB1 acetylation.