腰椎间盘突出症CT影像与治疗方式选择

本文报导了２６例腰椎间盘突出的症神经阻滞治疗效差病人的ＣＴ影象及治疗方式，并将其分为３种主要情况；（１）早期创伤性反应释放出致痛物质刺激神经根，ＣＴ表现神经根明显肿胀，２个以下层面的椎间盘膨出；（２）病程全长，神经根受压明量及粘连形成，ＣＴ表现２个以上层面椎间盘突出；（３）手术后复发的ＣＴ影像，Ａ软组织嵌入椎反开窗的，久之粘连及纤维化，压迫和牵拉伸经根，Ｂ与手术部位相邻的椎间盘再突出，处理原则，第     

Rosiglitazone down-regulates lipopolysaccharide-induced expression of CD40 and intercellular adhesion molecule 1 in rat peritoneal mesothelial cells through a NF-κB dependent mechanism

Objective To investigate the effect and mechanism by which PPARγ ligand, rosiglitasone, regulates the expression of CD40 and intercellular adhesion molecule 1 (ICAM-1) in the rat peritoneal mesothelial cells (RPMCs) induced by lipopolysaccharide (LPS). Methods RPMCs were harvested from Sprague-Dawley rat peritoneal cavity and maintained under defined in vitro conditions. The cells were randomly divided into groups as follows: medium, LPS (5 mg/L), LPS (5 mg/L)+BAY11-7085(5 μmol/L, NF-κB inhibitor), rosiglitazone (10 μmol/L or 20 μmol/L, peroxisome proliferator-activated receptor γ activator), LPS (5 mg/L)+rosiglitazone (10 μmol/L)+GW9662 (3 μmol/L, peroxisome proliferator-aetivatcd receptor γ antagonist), and LPS (5 mg/L)+vehicle (DMSO 0.2 ml/L). The expressions of CD40 and ICAM-1 RNA in RPMCs were examined by RT-PCR after 3 hour treatment, and the protein expressions of CD40, ICAM-1, p-NF-κB p65 and p-IκBα were examined by Western blot or immunofluorescence after 24 hour treatment. Results Following treatment with LPS, both the expressions of CD40 and ICAM-1 protein in RPMCs were up-regulated significantly (P<0.05), and the phosphoralation of p65 was increased greatly (1.10±0.17 vs 0.55±0.06, P<0.05). BAY11-7085 (5 μmol/L) significantly decreased the protein expression of p-p65 (0.22±0.11 vs 1.10±0.17, P<0.01), CD40 (0.34±0.02 vs 0.50±0.06, P<0.05) and ICAM-1 (0.35±0.16 vs 0.74±0.03, P<0.05). Pretreated with rosiglitazone for 3 h then added with LPS for 1 h, the levels of p-p65, CD40 and ICAM-1 in RPMCs were significantly decreased compared with those of LPS group (0.77±0.08 vs 0.90±0.10, P相似文献   

骨折复位固定器治疗股骨转子间骨折25例报告

1984．9～1985．4月，我们采用骨折复位固定器治疗股骨转子间骨折25例（包括2例股骨颈基底部骨折），现报告如下。     

医院内部卡的选择与使用

随着各医院信息化建设的进一步发展，很多医院正在逐步推行医院内部卡。医院内部卡的使用使得医院可以监控患者就诊的大部分流程，增大管理的力度与效果，同时还可以在一定程度上方便患者，减少患者排队等候的时间，因此正在逐步推广。北京同仁医院在2003年12月也推出了自己的院内卡，取得了较好的效果。在选     

非水性固定剂对大鼠腹膜表面层固定效果的观察

目的　比较水性和非水性固定剂对腹膜表面层超微结构的影响。方法　 2 0 0 3- 0 5 2 0 0 3- 12在中山大学附属第一医院取正常SD大鼠腹膜组织 ,分别以不同固定液固定 :(1) 2 5 %戊二醛和 2 %多聚甲醛 (对照组 ) ;(2 ) 4 %四氧化锇 (OsO4) (Os组 ) ;(3) 1%OsO4的氟化碳 (FC75)固定液 (FC组 )。结果　对照组中可见腹膜间皮细胞表面的微绒毛 ,未见腹膜表面层。OS组可见腹膜表面层 ,此层质地疏松 ,平均厚度 4 μm左右。FC组间皮细胞表面可见一层连续的腹膜表面层 ,其质地较OsO4组明显致密 ,厚薄较均匀平均 4 μm左右 ,表面较光滑。 结论　正常大鼠腹膜表面覆盖着一层腹膜表面层 ,非水性的含OsO4的FC75固定液是固定该表面层的良好固定剂     

腹腔内液体容量对腹膜转运功能的影响

目的探讨腹腔内液体容量对腹膜转运功能的影响。方法将18只雄性SD大鼠随机分成对照组(Con组,n＝6)、低容量组(LV组,n＝6)和高容量组(HV组,n＝6)。对照组未予任何处理;LV组和HV组腹腔内每次分别注射4.25％葡萄糖透析液25ml和40ml,每天1次,连续7d。停止注射48h后,LV组和HV组连同Con组的大鼠一起行腹膜动力学试验,并取腹膜组织行病理检查(HE染色)。结果与对照组相比,LV组和HV组大鼠的腹腔内液体重吸收率和淋巴重吸收率均明显增加,超滤量明显减少,溶质转运增加;但LV组和HV组的腹腔内液体量、腹腔内液体重吸收率及溶质转运均无显著性差异,唯HV组的淋巴重吸收率［(0.015±0.001)ml/min］高于LV组(0.010±0.003,P＜0.05);LV组和HV组的腹膜组织均呈慢性透析改变。结论4.25％葡萄糖透析液腹腔内注射7d使腹膜的通透性增高,但增加透析液容量除增加淋巴重吸收率外,并不加重腹膜功能的损害。淋巴重吸收率的增加可能是腹膜对高透析容量的一种代偿反应。     

Rosiglitazone down-regulates lipopolysaccharide-induced expression of CD40 and intercellular adhesion molecule 1 in rat peritoneal mesothelial cells through a NF-κB dependent mechanism

Objective To investigate the effect and mechanism by which PPARγ ligand, rosiglitasone, regulates the expression of CD40 and intercellular adhesion molecule 1 (ICAM-1) in the rat peritoneal mesothelial cells (RPMCs) induced by lipopolysaccharide (LPS). Methods RPMCs were harvested from Sprague-Dawley rat peritoneal cavity and maintained under defined in vitro conditions. The cells were randomly divided into groups as follows: medium, LPS (5 mg/L), LPS (5 mg/L)+BAY11-7085(5 μmol/L, NF-κB inhibitor), rosiglitazone (10 μmol/L or 20 μmol/L, peroxisome proliferator-activated receptor γ activator), LPS (5 mg/L)+rosiglitazone (10 μmol/L)+GW9662 (3 μmol/L, peroxisome proliferator-aetivatcd receptor γ antagonist), and LPS (5 mg/L)+vehicle (DMSO 0.2 ml/L). The expressions of CD40 and ICAM-1 RNA in RPMCs were examined by RT-PCR after 3 hour treatment, and the protein expressions of CD40, ICAM-1, p-NF-κB p65 and p-IκBα were examined by Western blot or immunofluorescence after 24 hour treatment. Results Following treatment with LPS, both the expressions of CD40 and ICAM-1 protein in RPMCs were up-regulated significantly (P<0.05), and the phosphoralation of p65 was increased greatly (1.10±0.17 vs 0.55±0.06, P<0.05). BAY11-7085 (5 μmol/L) significantly decreased the protein expression of p-p65 (0.22±0.11 vs 1.10±0.17, P<0.01), CD40 (0.34±0.02 vs 0.50±0.06, P<0.05) and ICAM-1 (0.35±0.16 vs 0.74±0.03, P<0.05). Pretreated with rosiglitazone for 3 h then added with LPS for 1 h, the levels of p-p65, CD40 and ICAM-1 in RPMCs were significantly decreased compared with those of LPS group (0.77±0.08 vs 0.90±0.10, P相似文献   

腹膜透析相关性真菌性腹膜炎20例临床分析

目的探讨腹膜透析相关性真菌性腹膜炎(FP)的易感因素、诊治和转归。方法回顾分析2000年1月～2005年11月期间中山大学附属第一医院肾内科收治的CAPD相关性真菌性腹膜炎的临床表现、病源菌、治疗、转归,并与同期收治的革兰氏阳性(G~ )球菌、革兰氏阴性(G~-)杆菌腹膜炎进行分组对比。结果腹膜透析相关性腹膜炎205例,其中真菌性腹膜炎20例(9．76%)；真菌性腹膜炎组、G~ 球菌组、G~-杆菌组3组间的发病年龄、性别、血白细胞计数、透出液细胞计数、开始透析时间差异无统计学意义(P＞0．05),真菌性腹膜炎组腹部压痛、反跳痛的发生率显著高于其余两组(P＜0．05)；17例(85%)患者1月内有腹腔/全身系统使用抗生素史：真菌性腹膜炎组6例(30%)死亡,其余14例转血液透析,死亡率及腹膜透析退出率显著高于其余两组(P＜0．05)。结论腹腔/全身使用抗生素是腹膜透析相关真菌性腹膜炎发生的高危因素,真菌性腹膜炎临床症状较重,预后不良,确诊后早期拔管可能可以降低死亡率。     

自我指压穴位按摩治疗失眠症的疗效观察

由于现代工作和生活节奏加快，失眠症的发病率呈上升趋势。2002年国际精神卫生和神经学基金会（IMFHN）调查资料显示，我国普通人群中有45．5％的人群存在失眠问题，住院患者中失眠症的发病率高于50％。我国卫生部2001年公布的统计资料显示，我国失眠症患者人数已达到120—240万人。     

医院内部卡的选择与使用

随着各医院信息化建设的进一步发展，很多医院正在逐步推行医院内部卡。医院内部卡的使用使得医院可以监控患者就诊的大部分流程，增大管理的力度与效果，同时还可以在一定程度上方便患者，减少患者排队等候的时间，因此止在逐步推广。北京同仁医院在2003年12月也推出了自己的院内卡，取得了较好的效果。在选择医院内部卡的过程中，我们比较了很多种方案，总结出了一些经验，介绍如下：