INDUCTION OF APOPTOSIS IN S-180 AND S-180R TUMOR CELLS BY ADRIAMYCIN IN VIVO

Apoptosis of tumor cells have become a newstandard for chemotherapy. It is useful to demonstrateinduction of apoptosis in tumor cells by anti-cancer drugsin vivo. We reported the results of apoptosis induction inmurine tumor cell line S-180 and it’s resistant cell linc S-180R by adriamycin in different dose and different time.We found that apoptosis in S-180 cells could be inducedby low dose of adriamycin, the apoptosis was started at 24h. after the administration, and reached to 62.5% of thecells to apptosis until 72 h. Comparison with theparental cell line, only 13% of S-180R cells wereapoptosed. At high dose, 20% of S-180R cells wereapoptosed, whereas, almost all S-180 cells were killed inthe same time. The lymphocytes were appeared inabdominal cavity of the mice after treatment ofadriamycin for 24 h. It was very interested to find outthat there was no lymphocyte left in the abdominal cavityof the mice with S-180R cells treated at high dose ofadriamycin.     

阿霉素在小鼠体内诱导S—180细胞株抗药性的实验研究

利用ＢＡＢＬ／Ｃ小鼠，腹腔接种Ｓ－１８０瘤细胞，阿霉素腹腔注射治疗１５个周期培育传代，得到抗阿霉素的Ｓ－１８０细胞株，此抗药细胞对阿霉素的抗药性比亲本细胞提高６６倍，对典型的ＤＮＡ拓扑异构醇Ⅱ抑制剂ＶＰ１６抗药性增加９倍，经免疫组化进一步证实，抗药细胞显示多药抗药基因产物Ｐ－１７０糖蛋白过表达，流式萤光细胞仪测试结果也表明抗药细胞比亲本细胞排出药物能力提高８９倍，可以肯定这是ＭＤＲ基因过表达产物所     

THE RELATIONSHIP BETWEEN cAMP AND cGMP IN REGIONS OF RAT BRAIN AND ACUPUNCTURE ANALGESIA

In this experiment,animals were sacrificedby total immersion in liquid nitrogen to fix thebrain tissue,cAMP and cGMP in brain areaswere determined by radioimmunoassay.Wecorrelated the levels of cAMP and cGMP in thebrain with acupuncture analgesia.Our resultsshow that acupuncture analgesia led to a rise ofcGMP in the rat diencephalon and lower brainstem,while the levels of cAMP in the rat brainareas were unchanged after acupuncture.     

DEVELOPMENT OF AN ADRIAMYCIN RESISTANT MURINE TUMOR S-180 CELL LINE IN VIVO

Adriamycin resistant cells were obtained from low dotage treated BABL/c mice Inoculated with S-180 cells. Resistance of these cells for adriamycin was 66-fold more than their parental cells. The resistance for a typical DNA topoisomerase Ⅱ inhibitor VP16 (Etopcaide) was increased 9 times. Overexpression of multidrug resistant gene (MDR gene) products, P-glycoproteins (P-1 70), was also demonstrated by immunohistochemistry. Furthermore, the ability of the resistant cells to reduce net cellular drug accumulation measured by flow fluorescence cytometry was 89-fold higher than their parental cells. These results support the hypothesis that the resistance of S-180R cells to adriamycin was mainly due to the overexpression of P-glycoproteins. The S-180R cells will be useful to select drugs or some other therapeutic strategies to overcome multidrug resistance in vivo.     

EFFECT OF ACUPUNCTURE ON THE ACTIVITIES OF PHOSPHODIESTERASESINRATBRAIN

The relationship between the activities of pho-sphodiesterases(PDEs)in brain regions and acupunc-ture analgesia were studied.Results showed that inacupuncture analgesia group,the activity of cAMP-PDE in the brain stem,diencephalon and striatumwas the same as in acupuncture non-analgesia,re-strained control,and normal control groups.But theactivity of cGMP-PDE in the brain stem of the acu-puncture analgesia group was decreased significant-ly(compared with that of the restrained controlgroup,P<0.01,and also compared with that of thenormal control and the acupuncture non-analgesiagroups P<0.05).The study suggested the possibilityof elevation of cGMP level in the brain stem as dueto the decreased activity of cGMP-PDE in this re-gion.     

艾灸治疗关节痛的疗效与神经组织内p-物质和脑啡肽的关系

艾灸对福氏佐剂引起的关节炎大鼠的关节痛有显著疗效。艾灸也有助于患鼠坐骨神经内异常P-物质含量的恢复;并能使腰髓内亮-脑啡肽和P-物质保持较高的水平。结果表明,艾灸能加强脊髓内脑啡肽系统的功能,并可能激活下行的P-物质能神经元,它们在调制一级传入神经元的功能方面起重要的作用,因此艾灸的上述作用可能与其治疗关节痛的疗效有关。     

甲基酪氨酸对实验性大鼠针刺镇痛作用的影响

<正> 很多实验证明中枢单胺类递质与针刺镇痛作用有关。我室对实验性电针镇痛的大鼠同时测定中枢单胺类递质表明,脑干的5-羟色脓增高,端脑去甲肾上腺素轻度下降。当用对氯苯丙氨酸耗竭脑内5-羟色胺时,可消除大鼠的针刺镇痛作用。北医针麻组也有类似的结果,而且证明5-羟色氨酸能加强针麻镇痛的效果。然而中枢儿茶酚胺在针刺镇痛中的作用比较复杂,实验报道较     

针刺对血浆环核苷酸的影响和针刺镇痛与血浆环核苷酸水平的关系

针刺在临床上能治疗和缓解多种疾患,其主要特点是激发或调整机体内在的生理功能,使之正常化,即中医所说的“激发精气”、“疏导气血”、“调和阴阳”。针刺疗法的这种特点表明,不同的机体状态,对针刺     

MOLECULAR BIOLOGICAL EVIDENCES FOR THE GENETIC STABILITY OF DOXORUBICIN RESISTANT CELL LINE S-180R IN VIVO

Ｔｈｅｄｒｕｇｒｅｓｉｓｔａｎｔｒｅｓｅａｒｃｈｅｓｉｎｖｉｖｏａｒｅｔｈｅｂｅｓｔｗａｙｔｏｕｎｄｅｒｓｔａｎｄｔｈｅｃｏｍｐｌｅｘｉｔｙｏｆｃｌｉｎｉｃａｌｄｒｕｇｒｅｓｉｓｔａｎｃｅ．Ｓ１８０Ｒｉｓｍｕｒｉｎｅａｓｃｉｔｅｓｔｕｍｏｒｃｅｌｉ...     

阿霉素在小鼠体内诱导S－180细胞株抗药性的实验研究

利用ＢＡＢＬ／ｃ小鼠，腹腔接种Ｓ－１８０瘤细胞，阿霉素腹腔注射治疗１５个周期培育传代，得到抗阿霉素的Ｓ－１８０细胞株（Ｓ－１８０Ｒ）。此抗药细胞对阿霉素的抗药性比亲本细胞提高６６倍。对典型的ＤＮＡ拓扑异构酶Ⅱ抑制刻ＶＰ１６（Ｅｔｏｐｏｓｉｄｅ）抗药性增加９倍。经免疫组化进一步证实，抗药细胞显示多药抗药基因（ＭＤＲｇｅｎｅ）产物Ｐ－１７０糖蛋白过表达。流式萤光细胞仪测试结果也表明抗药细胞比亲本细胞排出药物能力提高８９倍。可以肯定这是ＭＤＲ基因过表达产物所致。本工作为筛选有效逆转抗药功能的药物及其他治疗手段提供了有用的动物模型。