Inhibition of PRRX2 suppressed colon cancer liver metastasis via inactivation of Wnt/β-catenin signaling pathway

The aim of this study was to investigate whether PRRX2 may regulate the liver metastasis of colon cancer via the Wnt/β-catenin signaling pathway. PRRX2 and β-catenin in patients with the liver metastases of colon cancer was detected by immunochemistry. Colon cancer cells (CT-26 and CMT93) were divided into Normal, si-Ctrl, si-PRRX2 and si-PRRX2 +LiCl groups. Cell invasive and migrating abilities and the related proteins were detected. Liver-metastatic mice model was constructed consisting of Normal, NC shRNA and PRRX2 shRNA groups to examine the function of PRRX2 shRNA on liver metastasis. We found that PRRX2 and β-catenin positive rate was elevated in colon cancer tissues, especially in those tissues with liver metastasis, and there was a close relation between PRRX2 and the clinical staging, lymph node metastasis and numbers of liver metastases of colon cancer patients with liver metastasis. In vitro, the invasive and migrating abilities of CT-26 and CMT93 cells decreased apparently in the si-PRRX2 group, with down-regulation of PRRX2, p-GSK3β^Ser9/GSK3β, nucleus and cytoplasm β-catenin, TCF4 and Vimentin but up-regulation of E-cadherin. However, LiCl, the Wnt/β-catenin pathway activator, can reverse the inhibitory effect of si-PRRX2 on invasive and migrating ability of colon cancer cells. In vivo, the volume and weight of transplanted tumor and the number of liver metastases in the PRRX2 shRNA group were significantly reduced, with the similar protein expression patterns as in vitro. In a word, PRRX2 inhibition may reduce invasive and migrating abilities to hinder epithelial-mesenchymal transition (EMT), and suppress colon cancer liver metastasis through inactivation of Wnt/β-catenin pathway.     

东乡族成年女性绝经前后骨强度和体成分特征及其相关性

目的 分析东乡族成年女性绝经前后骨强度和体成分变化并探讨体成分变化对骨强度的影响。 方法 2016年9月~2018年7月采用随机整群抽样选取甘肃省东乡族41～50岁成年女性203例（绝经前102例,绝经后101例）,采用超声骨密度仪、生物电阻抗分析仪分别测量跟骨骨强度和体成分指标。 结果 东乡族绝经后女性的骨强度、肌肉组织体成分低于绝经前（P<0.05）;脂肪组织体成分高于绝经前（P<0.01）。东乡族绝经后女性骨质疏松的患病率高于绝经前（P<0.01）。Pearson 相关分析显示,绝经前后女性的骨强度均与肌肉组织体成分正相关（P<0.01）,与脂肪组织体成分负相关（P<0.01）。多元线性逐步回归分析显示,四肢肌肉量是东乡族女性骨强度的保护性因素,皮下脂肪含量是骨强度下降的危险因素。 结论 肌肉以及脂肪组织共同决定着东乡族女性的骨强度且与体成分分布部位有关,但两者的关系不受绝经状态的影响。绝经是东乡族女性骨质疏松发生的重要影响因素,应加强绝经后女性骨质疏松的防控。加强肢体锻炼,增加四肢肌肉量,减少皮下脂肪,有助于提高东乡族绝经前、后女性骨强度,预防骨质疏松。     

甘肃省急诊护理人员心理健康状况调研及分析

目的:分析甘肃省急诊护理人员心理健康现况,探讨科学的管理和改进方法。方法:采用SCL-90量表,随机选取甘肃省30家医疗机构(包括院前医疗急救机构)的急诊护理人员,按照资料类别分为省级医院、兰州市级医院、地州级医院及县级医院4个组,进行系统全面调查,了解甘肃省急诊护理人员心理健康状况并予以统计学分析,提出合理化建议。结果:1/3的甘肃省急诊护理人员存在不同程度心理问题,尤以强迫症状突出,占到总人数的37.0%。女性的心理健康状况较男性更差,地州级医院急诊护理人员心理不良倾向更为突出。结论:甘肃省急诊护理人员心理状况堪忧,各级医院领导层和护理管理者要重视急诊护理人员的心理卫生健康状况,通过多种途径积极减轻压力,维护急诊护理人员的心理健康。     

Epidemiological study of the end stage renal disease patients in Gansu province

Objective To investigate the clinical features and treatment of the end stage renal disease (ESRD) patients in Gansu province. Methods Based on the Chinese national renal data system, the investigation and analysis were made on the epidemiological literature of ESRD patients in 22 hospitals of Gansu from 2012 to 2013 by retrospective investigation. Results (1) In Gansu, the number of living ESRD patients was 4379, the point prevalence rate of ESRD was 169.6 per million. Their average age was (47.46±15.57) years, 30 to 59 years old patients accounted for 70.0%, and the male-female ratio was 1:1.15. The prevalence rate was higher in less-educated population and manual laborers. (2) As the leading cause of ESRD, chronic glomerulonephritis accounted for 43.0%, followed by diabetic nephropathy (31.0%), hypertensive nephropathy (11.0%) and allergic diseases (6.9%). (3) The current treatment of ESRD: 51.2% of the patients received hemodialysis, 4.4% received peritoneal dialysis, 1.1% received renal transplantation, 32.2% received no treatment, and 11.1% died. (4) The causes of patients not taking dialytic treatments: economic reasons accounted for 61.0%, lack of blood dialytic conditions accounted for 24.0%, patients ceasing treatment accounted for 3.1%, family factors accounted for 2.3%, religious reasons accounted for 1.8%, other reasons accounted for 7.8%. Conclusions The point prevalence rate of ESRD in Gansu was 169.6 per million. 30 to 59 years old patients were the main population. The major cause of ESRD was chronic glomerulonephritis, followed by diabetic nephropathy and hypertensive nephropathy. 32.2% of ESRD patients did not receive any renal replacement therapy, which was caused mainly by economic difficulties and the lack of dialytic equipments.     

2010-2018年甘肃省胃癌发病死亡比较分析

目的:分析2010-2018年全球、亚洲、东亚地区、中国、甘肃省胃癌发病、死亡情况。为中国及甘肃省胃癌预防控制工作提供流行病学依据。方法:中国人口标准化率(中标率)采用2000年全国人口普查的标准人口年龄构成,世界人口标准化率(世标率)采用Segi's标准人口年龄构成。由GLOBOCAN数据库获得全球、亚洲、东亚地区、中国的胃癌世标发病率、死亡率。由国家癌症中心公布的统计数据获得2010-2014年中国胃癌中标发病率及死亡率。由甘肃省肿瘤登记处上报的数据获得甘肃省胃癌中标发病率及死亡率。分别分析五个地区层次胃癌发病率、死亡率的变化趋势,并对5个地区的胃癌发病率、死亡率进行比较。结果:各地区胃癌的发病率、死亡率均呈逐年下降趋势;亚洲及东亚地区胃癌发病率、死亡率较全球的高,中国胃癌发病率、死亡率较亚洲的高,甘肃省胃癌发病率、死亡率较中国的高。结论:甘肃省胃癌的发病率、死亡率明显高于全国平均水平。今后胃癌的防治仍需作为中国尤其是甘肃省恶性肿瘤预防控制工作的重点。     

MicroRNA profiles in five pairs of early gastric cancer tissues and adjacent non-cancerous tissues

Approximately half of the world''s gastric cancer cases and deaths occur in China. In addition, the incidence and mortality rates of gastric cancer in Gansu province in China are much higher than the average nationwide levels. The present study investigated microRNA (miRNA/miR) profiles in early gastric cancer (EGC) without specific symptoms. miRNA expression levels in five pairs of EGC tissues and adjacent non-cancerous mucosa tissues of patients from Gansu province in China were analyzed using a miRNA microarray. A total of 47 differentially expressed miRNAs (DEMs) were identified. Subsequently, mRNA expression profiles of three pairs of cancer tissues and adjacent non-cancerous tissues from 3 Asian patients with stage I or stage II gastric cancer (stage I/II; American Joint Committee on Cancer classification, Eighth Edition) were obtained from The Cancer Genome Atlas database, and differentially expressed genes (DEGs) were identified. The target genes of DEMs were filtered from the DEGs using the miRDB database and a miRNA-gene network was constructed. The functions of DEMs were evaluated using the tool for annotations of human miRNAs database, and via Gene Ontology analysis, Kyoto Encyclopedia of Genes and Genomes analysis and Gene Set Enrichment Analysis of the target genes. Finally, survival analyses of DEMs, which were in the miRNA-gene network, was performed. The results suggested that a number of miRNAs, including hsa-let-7a-5p, hsa-miR-27a-3p, hsa-miR-126-5p and hsa-miR-424-5p, may serve critical roles in EGC. The present study could provide a basis for the identification of EGC screening biomarkers. Furthermore, the present study may provide a basis for the exploration of the cause of the high incidence of gastric cancer in Gansu province from the perspective of miRNAs.     

2009—2015年甘肃省肿瘤登记地区胰腺癌流行特征及变化趋势分析

目的 分析甘肃省肿瘤登记地区2009—2015年胰腺癌发病与死亡的流行特征及变化趋势,为制定胰腺癌的防治措施提供依据。方法 按照全国肿瘤登记中心制订的审核方法和评价标准,评估甘肃省11个肿瘤登记地区上报的胰腺癌数据。分地区、性别、年龄别计算胰腺癌发病(死亡)率、中标发病(死亡)率、世标发病(死亡)率、0~74岁累积率及构成。运用年度变化百分比(Annual percentage change,APC)对胰腺癌中标发病(死亡)率进行时间趋势分析。结果 2009—2015年甘肃省肿瘤登记地区胰腺癌新发病例1 963例,发病率为5.18/10万(男性6.01/10万,女性4.31/10万),中标发病率为4.67/10万,世标发病率5.44/10万。同期胰腺癌死亡病例1 708例,死亡率为4.51/10万(男性5.26/10万,女性3.72/10万),中标死亡率为3.78/10万,世标死亡率4.49/10万。农村地区中标发病率和中标死亡率(5.32/10万,4.63/10万)均高于城市地区(4.60/10万,3.54/10万)。胰腺癌发病率和死亡率均随年龄增长而上升,呈现男性高于女性的特点;2009—2015年甘肃省胰腺癌中标发病率和中标死亡率均呈现平稳趋势(APC=-4.2%,t=-1.6,P=0.2和APC=-3.6%,t=-1.1,P=0.1)。结论 2009—2015年甘肃省胰腺癌的发病和死亡虽呈平稳趋势,但农村地区高于城市地区,其中中老年男性是胰腺癌的重点防控人群,应加强胰腺癌的病因学研究和早期诊断,有效控制胰腺癌的发生发展。     

2009—2015年甘肃省肿瘤登记地区甲状腺癌流行特征及变化趋势

目的 分析甘肃省肿瘤登记地区2009—2015年甲状腺癌发病与死亡的流行状况。方法 审核整理2009—2015年甘肃省肿瘤登记地区甲状腺癌发病(死亡)例数、发病(死亡)率、构成比、中标率、世标率等,分析年龄别发病、死亡情况,并通过Jointpoint回归模型计算年度变化百分比(Annual percentage change,APC)进行时间趋势分析。结果 甘肃省2009—2015年肿瘤登记地区甲状腺癌报告发病1 087例,发病率为2.87/10万,中标率为2.51/10万;死亡率为0.86/10万,中标率0.80/10万;发病率和死亡率为女性高于男性,农村高于城市的特征;发病率和死亡率随年龄增长而增加;2009—2015年甲状腺癌中标发病率及死亡率均呈现上升趋势(APC=10.6%,t=2.4,P=0.10;APC=7.0%,t=1.3,P=0.30),趋势变化无统计学意义。结论 2009—2015年甘肃省甲状腺癌症发病率和死亡率呈现较高水平,城市50岁以上女性是甲状腺癌防控的重点对象。     

基于ISSR的甘肃不同产区栽培当归遗传多样性研究

目的研究甘肃不同产区栽培当归的遗传多样性。方法应用ISSR分子标记技术对甘肃省41个居群的栽培当归样本进行分析,利用Popgene 32软件分析Nei’s基因多样性指数(H)等遗传信息参数,应用Ntsys软件构建亲缘关系UPGMA聚类图。结果 8条引物共检测到154个位点,其中多态性位点119个,多态位点百分率(PPB)为77.27%。栽培当归居群间的H为0.222 9,Shannon’s多样性信息指数(I)为0.337 4,种群间基因分化系数(Gst)为0.683 9,基因流(Nm)为0.2311,遗传距离变化范围0.042 9~0.327 8。结论甘肃栽培当归遗传多样性在物种水平上较高;居群间遗传多样性水平明显高于居群内;居群间遗传分化程度大,且基本无基因交流。     

甘肃省康乐县药用植物资源初探

目的:进一步摸清甘肃省康乐县野生药用植物资源及其分布规律,分析药用植物多样性的特征,为该县中草药资源的开发利用和保护提供科学依据。方法:通过野外考察、标本采集、鉴定与资料考证相结合的方法对甘肃省康乐县药用植物资源多样性进行研究。结果:康乐县现有野生药用植物65科258种,以菊科和蔷薇科为主,其中国家级重点药用品种24科39属43种。该地野生药用植物的药用部位主要为全草类和根或根茎类,分别占40.31%和25.19%,药性主要以清热药和祛风湿药为主,分别占28.68%和12.79%。现有栽培药用植物12种,栽培面积达3 000公顷。结论:康乐县药用植物资源虽然丰富,但是多数品种蕴藏量不大,而且近年中草药资源也在逐渐减少,所以应在保护的前提下加快研究进度,进行合理开发利用。