FDP(1,6-二磷酸果糖)可减轻急性心肌梗塞再灌注对心肌的损害

在动物实验和临床研究中业已证实,FDP(Esafosfina,BiomedicaFos Cama Ita)对急性心肌梗塞及休克有肯定的治疗作用。为进一步探讨FDP对急性心肌梗塞再灌注引起的心肌损害有否相同的治疗作用,本文选用了28条麻醉狗,将其左前降支主干(LCA)结扎2小时,然后进行再灌注。治疗组为14条狗,于再灌注前5分钟分别通过静脉输注75mg/kgFDP(10％浓度),然后进以6mg/kg/min的速度维持滴注FDP至总量达500mg/kg。另外14条实验狗为对照组,在相同条件下改以静脉输注相同剂量的葡萄糖生理盐水。再灌后2小时存活的实验狗任其复苏,并于24小时后处死以获取心肌梗塞面积的解剖学依据。     

模拟调强技术照射鼻咽低分化鳞癌细胞的生物效应机制研究

Objective To study the altered radiobiological effect of simulative intensity-modulated radiotherapy (SIMR) in cultured human nasopharyngeal carcinoma (NPC) cells and the related mechanism. Methods Single cell suspension of exponentially growing CNE-2 cells, a poor differentiated NPC cell line, was seeded and cultured for 12 hours, then the cells were irradiated in two different models by 6 MV X-ray beams at 3 Gy/min. In single fraction irradiation (SFR) model, cells were irradiated a single fraction of 0, 2, 4, 6 or 8 Gy within 0 to 3 minutes. In S1MR model, cells were irradiated 0, 2, 4, 6 or 8 Gy in 5 frac-tions with interval of 8.0-8.5 minutes between. Clonogenic assay was performed to determine the radiosen-sitivity. Cellular apoptosis was measured by flow cytometry. RT-PCR was used to detect mRNA expressions of Bax and Bcl-2, Respectively. Results Compared with SFR group, the survival fraction in SIMR group was higher at all the dose levels. The values of α, β, D0 and Dq were higher in SIMR group than in SFR group. At dose levels of 2 Gy, 4 Gy and 6 Gy, The early and late apoptotic cells in SIMR group were lower than in SFR group (21.20%: 15.89%, F=18.51, P=0.020;13.00%: 10.20, F=15.67, P=0.040).The mRNA expression of Bax was upregulated in a dose-dependent manner in the both groups. Compared with SFR group, the mRNA expression of Bax in SIMR group was lower at all the dose levels (Mean value of 76.75% : 62.50%, F =36.57, P =0.000). Bcl-2 mRNA expression at every dose level had no significant difference between the two groups (Mean value of 29.25% : 29.75%, F=0.74, P=0.800). Conclusions Prolonged delivery time in SIMR model can decrease the radiobiological effects.     

超声引导下乳腺穿刺组织冰冻切片检查的临床应用

目的 探讨对超声引导下穿刺的乳腺组织进行快速冰冻切片检查、以得到病理学诊断的可行性和临床应用价值。方法 使用BIOPINCE穿刺活检枪,超声引导下对31例乳腺疾病患者行病灶的组织穿刺,并即刻将获取标本行冰冻切片检查。结果 30例患者给出明确诊断,1例诊断不确定,诊断准确率为96.8％;自穿刺开始到得出病理学诊断,所需平均时间为56.3min。结论 超声引导下乳腺穿刺组织冰冻切片检查具有诊断准确率高,快捷,微创,无明显疤痕,患者乐意接受,且勿须增加新设备等优势,有临床应用价值。但应注意使用的适应证。     

乳房穿刺组织快速冰冻切片检查的临床应用

我们尝试在超声影像引导下 ,以自动活检枪穿刺、获取乳房病变组织 ,并将获取标本立即行冰冻切片检查。现将临床开展情况总结如下。资料与方法一、一般资料2 0 0 2年 1月至 2 0 0 3年 3月 ,用自动活检枪对 2 2例女性乳房疾病患者行超声引导下乳房穿刺组织快速冰冻切片检查。患者平均年龄 34 7岁 ,既往均做过彩色多普勒超声检查 ,部分还做过近红外光扫描、钼靶X线、乳房导管造影、CT、MRI中的 1～ 2项检查 ,甚至有 2例做了PET检查。二、活检方法据病变部位 ,患者取仰卧位或侧卧位。超声下选定距离病变最短的进针方向和角度 ,体表做标记。…     

经阴道超声引导卵巢电凝打孔治疗多囊卵巢综合征性不孕症

目的探讨经阴道超声引导卵巢未成熟卵泡抽吸后电凝打孔术对多囊卵巢综合征(PCOS)性不孕症的疗效。方法选择38例PCOS不孕症患者,采用经阴道超声引导穹隆穿刺,卵巢未成熟卵泡抽吸后电凝打孔术。检测治疗前后血清黄体生成素(LH)、促卵泡激素(FSH)、睾酮(TTE);观察治疗后排卵率、妊娠率等指标。结果治疗后血清LH、TTE、LH/FSH值降低,FSH值升高,与治疗前比较差异均有统计学意义;治疗后排卵率为73.7%、妊娠率为57.9%。结论该电凝术治疗PCOS不孕症具有妊娠率高,手术操作简单,并发症少等优点,是克罗米芬(CC)治疗PCOS失败后有效的治疗方法。     

壳聚糖与生长因子复合贴剂对创面愈合的疗效观察

目的探讨壳聚糖与生长因子为主的复合贴剂,对大鼠感染创面的治疗效果.方法用Wistar大鼠制作创面感染模型,分别以壳聚糖与生长因子的复合贴剂(实验一组)和生长因子喷雾剂(实验二组)进行创面换药,对照组仅以生理盐水清洗创面.结果⑴对照组和实验一、二组创面愈合时间分别为(15.60±0.55)d、(11.90±0.45)d和(13.65±0.45)d;⑵在创面肉芽组织出现时间和创面分泌物减少时间两方面,实验一组明显较对照组和实验二组缩短,差异有显著性(P﹤0.05).结论⑴壳聚糖具有较明显的抗菌、消炎、减少创面渗出之功能,为生长因子充分发挥促细胞增殖作用提供了基础;⑵壳聚糖复合贴剂覆于创面可形成保护薄膜,使创面在换药、愈合过程中免于再损伤.     

小檗碱通过破坏线粒体功能选择性诱导淋巴瘤细胞的凋亡

目的研究小檗碱(BBR)对T淋巴瘤细胞凋亡的影响及机制。方法正常T淋巴细胞、T淋巴瘤细胞、Jurkat细胞用BBR 0,10,20和40μmol·L^-1处理24 h,多柔比星(Dox)40μmol·L^-1为阳性对照;Jurkat p0细胞用BBR 0和40μmol·L^-1处理24 h。流式细胞术检测细胞凋亡率,Seahorse XF24细胞代谢分析仪和H2DCFDA活性氧探针检测细胞氧消耗率(OCR)和活性氧(ROS)水平。CellTiter-Glo发光法细胞活力检测试剂盒检测细胞ATP水平,试剂盒测线粒体呼吸链复合物Ⅰ,Ⅱ,Ⅳ和Ⅴ的活性。Western印迹法检测细胞内胱天蛋白酶3、Bcl-2、Bax、NF-κB抑制蛋白激酶α/β(IKKα/β)、磷酸化IKKα/β(p-IKKα/β)、NF-κB抑制因子α(IκBα)、p-IκBα和P65蛋白表达水平。结果BBR 40 mmol·L^-1明显增加T淋巴瘤细胞和Jurkat细胞凋亡率(P<0.01),胱天蛋白酶3和Bax蛋白表达水平显著上调(P<0.01),Bcl-2表达显著下调(P<0.01),但对正常T淋巴细胞的凋亡并无影响;BBR 40 mmol·L^-1明显降低Jurkat细胞的OCR及线粒体呼吸链复合物Ⅰ活性(P<0.01),增加ROS水平(P<0.01),降低ATP水平(P<0.01),但不影响线粒体缺陷型淋巴瘤Jurkat p0细胞呼吸链和凋亡;BBR 40μmol·L-1明显下调Jurkat细胞中NF-κB通路相关蛋白p-IKKα/β/IKKα/β、p-IκBα/IκBβ和核内P65表达(P<0.01)。结论BBR通过破坏线粒体功能选择性诱导淋巴瘤T细胞的凋亡,可能与抑制NF-κB通路有关。     

男性腹膜后巨大畸胎瘤一例报告

1病例报告患者男,38岁。2009年无明显诱因出现上腹部腹胀,左上腹部似可触及巨大肿物。彩超示腹腔内巨大混合性团块。2018年7月就诊于我院,完善相关检查:血生化均无异常,腹部CT提示腹膜后囊实性混杂密度影巨大占位(图1),位于胰腺后方并挤压胰腺向上移位,提示肿块与胰腺关系密切。大小约129mm×89mm×118mm。行手术治疗。     

交锁髓内钉合侧方钢板内固定与可桥接钢板内固定治疗下肢骨干无菌性骨不连的临床效果对比研究

目的 比较交锁髓内钉合侧方钢板内固定治疗与可桥接钢板内固定治疗下肢骨干无菌性骨不连的临床效果.方法 随机选取2011年10月～2013年11月在本院骨科手术治疗下肢骨干无菌性骨不连手术的256例患者为研究对象,根据治疗方法不同分成两组,交锁髓内钉组142例采用交锁髓内钉合侧方钢板内固定治疗,可桥接钢板组114例采用可桥接钢板内固定治疗,对比两组的围术期及术后随访情况.结果 术后256例患者骨折愈合且无并发症.交锁髓内钉组出血量和引流血量多于可桥接钢板组,内固定时间、临床愈合时间、影像愈合时间、住院时间长于可桥接钢板组,手术时间短于可桥接钢板组,骨痂评分低于可桥接钢板组,住院费用高于可桥接钢板组,差异均有统计学意义（P＜0.05）.结论 两种下肢骨干骨不连的临床治疗方法均取得较好疗效,临床医生可根据患者的既往创伤程度、经济水平以及医院现有技术等综合考虑,选择最有利的治疗方式.     

模拟调强技术照射鼻咽低分化鳞癌细胞的生物效应机制研究

Objective To study the altered radiobiological effect of simulative intensity-modulated radiotherapy (SIMR) in cultured human nasopharyngeal carcinoma (NPC) cells and the related mechanism. Methods Single cell suspension of exponentially growing CNE-2 cells, a poor differentiated NPC cell line, was seeded and cultured for 12 hours, then the cells were irradiated in two different models by 6 MV X-ray beams at 3 Gy/min. In single fraction irradiation (SFR) model, cells were irradiated a single fraction of 0, 2, 4, 6 or 8 Gy within 0 to 3 minutes. In S1MR model, cells were irradiated 0, 2, 4, 6 or 8 Gy in 5 frac-tions with interval of 8.0-8.5 minutes between. Clonogenic assay was performed to determine the radiosen-sitivity. Cellular apoptosis was measured by flow cytometry. RT-PCR was used to detect mRNA expressions of Bax and Bcl-2, Respectively. Results Compared with SFR group, the survival fraction in SIMR group was higher at all the dose levels. The values of α, β, D0 and Dq were higher in SIMR group than in SFR group. At dose levels of 2 Gy, 4 Gy and 6 Gy, The early and late apoptotic cells in SIMR group were lower than in SFR group (21.20%: 15.89%, F=18.51, P=0.020;13.00%: 10.20, F=15.67, P=0.040).The mRNA expression of Bax was upregulated in a dose-dependent manner in the both groups. Compared with SFR group, the mRNA expression of Bax in SIMR group was lower at all the dose levels (Mean value of 76.75% : 62.50%, F =36.57, P =0.000). Bcl-2 mRNA expression at every dose level had no significant difference between the two groups (Mean value of 29.25% : 29.75%, F=0.74, P=0.800). Conclusions Prolonged delivery time in SIMR model can decrease the radiobiological effects.