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目的 探讨缺氧诱导因子-1α(hypoxia inducible factor-1,HIF-1α)和BCL-2/腺病毒E1B19KDa相关蛋白3(Bcl2/adenovirus E1B 19 kD interacting protein 3,BNIP3)
在中耳胆脂瘤中的表达及胆脂瘤上皮的凋亡情况。方法 采用免疫组织化学方法检测30例中耳胆脂瘤标本与18例外耳道皮肤标本中HIF-1α和BNIP3蛋白的表达情况,使用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记(terminal deoxynucleotidyl transferase-mediated dUTP nick-endlabeling,Tunel)检测20例中耳胆脂瘤标本和18例外耳道皮肤标本的凋亡情况。使用Pearson相关分析检验HIF-1α和BNIP3蛋白之间的相关性。结果 HIF-1α在胆脂瘤组和对照组的平均光密度分别为0.16±0.07和0.08±0.03,两组比较差异有统计学意义(t =4.279,P<0.01);BNIP3在胆脂瘤组和对照组的平均光密度分别为0.16±0.08和0.11±0.06,两组比较差异有统计学意义(t =2.463,P =0.0185);经pearson相关分析,在胆脂瘤上皮中,HIF-1α和BNIP3之间呈正相关(r =0.418,P =0.003);Tunel染色中,凋亡指数在胆脂瘤组和对照组分别为(52.8±12.5)%和(9.99±2.97)%,两组比较差异有统计学意义(t =14.166,P<0.01)。结论 HIF-1α和BNIP3在中耳胆脂瘤中的异常表达可能与胆脂瘤的高凋亡特性有关。 相似文献
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人参皂甙等清除超氧阴离子自由基的研究 总被引:7,自引:0,他引:7
筛选自由基清除剂常用黄嘌呤氧化酶氧化黄嘌呤产生O_2~-·,但此体系中黄嘌呤氧化酶易被药物抑制,致使结果分不清是由于药物清除O_2·~-的作用还是药物抑制黄嘌呤氧化酶而 相似文献
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Recent studies indicated that interleukin(IL)-17, growth-related oncogene(GRO)-α and IL-8 play an important role in the pathogenesis of nasal polyps. However, the effects of the increased amount of IL-17 and the production of GRO-α and IL-8 in human nasal polyp fibroblasts are not completely understood. This study aimed to determine the effects of the increased IL-17 on the changes of GRO-α and IL-8 expression in human nasal polyp fibroblasts and further investigate the mechanism of neutrophil infiltration in nasal polyps. Nasal polyp fibroblasts were isolated from six cases of human nasal polyps, and the cells were stimulated with five different concentrations of IL-17. Real-time fluorescence quantitative polymerase chain reaction(RT-PCR) was used to detect the mRNA expression of GRO-α and IL-8. The mRNA of GRO-α and IL-8 was expressed in unstimulated controls and remarkably increased by stimulation with IL-17. Moreover, the levels of GRO-α and IL-8 produced by fibroblasts were increased gradually with the increases in IL-17 concentrations. The present study showed that nasal fibroblasts can produce GRO-α and IL-8, and their production is remarkably enhanced by IL-17 stimulation, thereby clarifying the mechanism of the IL-17 mediated neutrophil infiltration in nasal polyps. These findings might provide a rationale for using IL-17 inhibitors as a treatment for nasal inflammatory diseases such as nasal polyps. 相似文献
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为了寻找对心肌缺血再灌注损伤具有保护作用的药物,并探索不同基团修饰对苯甲酰胍与曲美他嗪偶联物活性的影响,将苯甲酰胍的4位通过亚甲基与曲美他嗪进行偶联,并在苯环的3位引入不同的取代苯甲酰胺基,设计合成了12个未见文献报道的目标化合物。其结构经过IR,1H NMR和MS确证。体外血小板肿胀模型(PSA)试验结果表明,化合物9b和9c抑制Na+/H+交换器-1(NHE1)的IC50分别为2.55和2.80 nmol/L,其活性是卡立泊来德(IC50=12.1 nmol/L)的4.7和4.3倍。 相似文献
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慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)是一种以气流受限不完全可逆,呈进行性发展为特征的呼吸系统疾患,与有害气体或有害颗粒(如吸烟)引起的肺部异常炎性反应有关.目前COPD是全球第4大致死原因,预计到2020年,将上升为第3位[1].抗炎治疗在COPD中具有重要作用,而现有的COPD抗炎药物不能有效阻止病情的进展,因此急需寻找新的抗炎治疗药物.随着对COPD病理机制的深入了解,已有一些新靶点的抗炎制剂开始进入COPD的治疗研究中.本文将对这些新型抗炎药物的研究进展做一综述. 相似文献