全文获取类型
收费全文 | 3504篇 |
免费 | 568篇 |
国内免费 | 95篇 |
专业分类
耳鼻咽喉 | 16篇 |
儿科学 | 95篇 |
妇产科学 | 34篇 |
基础医学 | 926篇 |
口腔科学 | 61篇 |
临床医学 | 456篇 |
内科学 | 355篇 |
皮肤病学 | 31篇 |
神经病学 | 211篇 |
特种医学 | 210篇 |
外国民族医学 | 2篇 |
外科学 | 175篇 |
综合类 | 440篇 |
预防医学 | 367篇 |
眼科学 | 53篇 |
药学 | 272篇 |
4篇 | |
中国医学 | 183篇 |
肿瘤学 | 276篇 |
出版年
2024年 | 7篇 |
2023年 | 101篇 |
2022年 | 84篇 |
2021年 | 216篇 |
2020年 | 190篇 |
2019年 | 220篇 |
2018年 | 191篇 |
2017年 | 167篇 |
2016年 | 205篇 |
2015年 | 163篇 |
2014年 | 246篇 |
2013年 | 269篇 |
2012年 | 201篇 |
2011年 | 191篇 |
2010年 | 150篇 |
2009年 | 155篇 |
2008年 | 136篇 |
2007年 | 160篇 |
2006年 | 139篇 |
2005年 | 125篇 |
2004年 | 102篇 |
2003年 | 91篇 |
2002年 | 75篇 |
2001年 | 67篇 |
2000年 | 59篇 |
1999年 | 59篇 |
1998年 | 35篇 |
1997年 | 57篇 |
1996年 | 25篇 |
1995年 | 28篇 |
1994年 | 23篇 |
1993年 | 22篇 |
1992年 | 27篇 |
1991年 | 16篇 |
1990年 | 16篇 |
1989年 | 20篇 |
1988年 | 23篇 |
1987年 | 8篇 |
1986年 | 8篇 |
1985年 | 18篇 |
1984年 | 10篇 |
1983年 | 12篇 |
1982年 | 20篇 |
1981年 | 9篇 |
1980年 | 8篇 |
1979年 | 5篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1975年 | 2篇 |
1970年 | 1篇 |
排序方式: 共有4167条查询结果,搜索用时 31 毫秒
1.
2.
3.
4.
Hildegunn Norbakken Granslo Elizabeth G. Aarag Fredheim Eirin Esaiassen Lars Christophersen Peter
strup Jensen Tom Eirik Mollnes Claus Moser Trond Flgstad Claus Klingenberg Jorunn Pauline Cavanagh 《APMIS : acta pathologica, microbiologica, et immunologica Scandinavica》2019,127(6):475-483
The global spread of antimicrobial resistance and the increasing number of immune‐compromised patients are major challenges in modern medicine. Targeting bacterial virulence or the human host immune system to increase host defence are important strategies in the search for novel antimicrobial drugs. We investigated the inflammatory response of the synthetic short antimicrobial peptide LTX21 in two model systems: a human whole blood ex vivo model and a murine in vivo peritoneum model – both reflecting early innate immune response. In the whole blood model, LTX21 increased the secretion of a range of different cytokines, decreased the level of tumour necrosis factor (TNF) and activated the complement system. In a haemolysis assay, we found 2.5% haemolysis at a LTX21 concentration of 500 mg/L. In the murine model, increased influx of white blood cells (WBCs) and polymorphonuclear neutrophils (PMNs) in the murine peritoneal cavity was observed after treatment with LTX21. In addition, LTX21 increased monocyte chemoattractant protein‐1 (MCP‐1). In conclusion, LTX21 affected the inflammatory response; the increase in cytokine secretion, complement activation and WBC influx indicates an activated inflammatory response. The present results indicate the impact of LTX21 on the host–pathogen interplay. Whether this will also affect the course of infection has to be investigated. 相似文献
5.
Lorena Martin-Morales Sara Manzano Maria Rodrigo-Faus Adrian Vicente-Barrueco Victor Lorca Gonzalo Núñez-Moreno Paloma Bragado Almudena Porras Trinidad Caldes Pilar Garre Alvaro Gutierrez-Uzquiza 《International journal of cancer. Journal international du cancer》2023,152(2):283-297
Matrix metalloproteinase-11 (MMP11) is an enzyme with proteolytic activity against matrix and nonmatrix proteins. Although most MMPs are secreted as inactive proenzymes and are later activated extracellularly, MMP11 is activated intracellularly by furin within the constitutive secretory pathway. It is a key factor in physiological tissue remodeling and its alteration may play an important role in the progression of epithelial malignancies and other diseases. TCGA colon and colorectal adenocarcinoma data showed that upregulation of MMP11 expression correlates with tumorigenesis and malignancy. Here, we provide evidence that a germline variant in the MMP11 gene (NM_005940: c.232C>T; p.(Pro78Ser)), identified by whole exome sequencing, can increase the tumorigenic properties of colorectal cancer (CRC) cells. P78S is located in the prodomain region, which is responsible for blocking MMP11's protease activity. This variant was detected in the proband and all the cancer-affected family members analyzed, while it was not detected in healthy relatives. In silico analyses predict that P78S could have an impact on the activation of the enzyme. Furthermore, our in vitro analyses show that the expression of P78S in HCT116 cells increases tumor cell invasion and proliferation. In summary, our results show that this variant could modify the structure of the MMP11 prodomain, producing a premature or uncontrolled activation of the enzyme that may contribute to an early CRC onset in these patients. The study of this gene in other CRC cases will provide further information about its role in CRC development, which might improve patient treatment in the future. 相似文献
6.
M.-D.-M. Amador F. Muratet E. Teyssou S. Boillée S. Millecamps 《Revue neurologique》2021,177(5):524-535
Due to novel gene therapy opportunities, genetic screening is no longer restricted to familial cases of ALS (FALS) cases but also aplies to the sporadic populations (SALS). Screening of four main genes (C9orf72, SOD1, TARDBP and FUS) identified the causes in 15% of Amyotrophic Lateral Sclerosis (ALS) patients (two third of the familial cases and 8% of the sporadic ones) but their respective contribution to ALS phenotype varies according the age of disease onset. The genetic overlap between ALS and other diseases is expanding and includes frontotemporal dementia, Paget's Disease of Bone, myopathy for adult cases, HSP and CMT for young cases highlighing the importance of retrieving the exhaustive familial history for each indivdual with ALS. Incomplete disease penetrance, diversity of the possible phenotypes, as well as the lack of confidence concerning the pathogenicity of most identified variants and/or possible oligogenic inheritance are burdens of ALS genetic counseling to be delivered to patients and at risk individuals. The multitude of rare ALS genetic causes identifed seems to converge to similar cellular pathways leading to inapropriate response to stress emphacising new potential therapeutic options for the disease. 相似文献
7.
《The Journal for Nurse Practitioners》2021,17(1):32-36
Despite health care reform, our nation continues to struggle containing health care costs while meeting the needs of persons with behavioral health disorders and comorbid chronic care conditions. The purpose of this article is to propose that dually certified primary care and psychiatric mental health nurse practitioners may be the disruptive innovation that becomes the solution for improving the coordination and care for high-need patients while containing costs. The concepts of disruptive innovation, integrated care, and whole person care are discussed followed by an introduction to the innovative primary care and psychiatric mental health nurse practitioner’s role and its potential for health care system disruption. Finally, a call to nursing is proffered to seize current opportunities to meet the Triple Aim challenge by providing whole person care that is high-quality, cost-effective, and satisfactory to patients because it is truly patient centered and meets their needs. 相似文献
8.
9.
Brandon Ginley Kuang-Yu Jen Seung Seok Han Luís Rodrigues Sanjay Jain Agnes B. Fogo Jonathan Zuckerman Vighnesh Walavalkar Jeffrey C. Miecznikowski Yumeng Wen Felicia Yen Donghwan Yun Kyung Chul Moon Avi Rosenberg Chirag Parikh Pinaki Sarder 《Journal of the American Society of Nephrology : JASN》2021,32(4):837
10.
目的探讨全身振动训练联合正弦交变电磁场对去卵巢骨质疏松大鼠骨密度、骨代谢、骨生物力学性能的影响。方法将100只大鼠随机分为切除卵巢组和对照组,分别进行卵巢切除术和假手术,大鼠进行6周恢复,恢复后将造模成功的切除卵巢大鼠随机分为模型组(MO组)、全身振动训练组(W组)、正弦交变电磁场组(S组)、全身振动训练+正弦交变电磁场组(WS组),对照组列为假手术组(SO组),进行为期16周干预,干预结束后对大鼠进行骨密度、骨代谢、骨生物力学性质的检测。结果16周干预完成后,MO组、S组大鼠体质量显著高于SO组、W组、WS组大鼠(P0. 05); W组、WS组、SO组大鼠骨密度指标、血清雌二醇浓度指标明显高于大鼠MO组(P0. 05); SO组大鼠血清雌二醇浓度指标明显高于S组、W组、WS组(P0. 05); M组大鼠血清OC、ALP浓度明显高于SO组、W组、S组、WS组大鼠(P0. 05);尿液DPD/Cre、Ca/Cre、P/Cre浓度方面,M组大鼠明显高于SO组、W组、S组、WS组大鼠(P0. 05)。SO组、W组、S组、WS组大鼠股骨最大载荷和弹性模型明显高于MO组大鼠(P0. 05)。SO组、W组、S组、WS组大鼠股骨断裂载荷组间无明显差异(P0. 05); SO组、W组、S组、WS组大鼠股骨弹性模量明显高于MO组大鼠(P0. 05)。SO组大鼠股骨弹性模量明显高于S组大鼠(P0. 05),但与W组、WS组大鼠没有明显差异(P0. 05)。L4椎体压缩试验,SO组、W组、S组、WS组大鼠股骨最大载荷和弹性模型明显高于MO组大鼠(P0. 05)。SO组大鼠高于S组、W组,差异有统计学意义(P0. 05),但与WS组大鼠没有明显差异(P0. 05)。结论全身振动训练、正弦交变电磁场、全身振动训练联合正弦交变电磁场3种干预方式施用于去卵巢骨质疏松大鼠均能提升骨密度、抑制骨吸收、平衡骨代谢、改善骨骼结构力学和材料力学性能。而全身振动训练联合正弦交变电磁场能的治疗效果优于单纯使用全身振动训练或正弦交变电磁场,在临床应用中有一定推广价值。 相似文献