Kombucha tea ameliorates experimental autoimmune encephalomyelitis in mouse model of multiple sclerosis

Experimental autoimmune encephalomyelitis (EAE) is a mouse model for multiple sclerosis (MS), in which an inflammatory demyelination and axonal damage occurs. Kombucha tea is a fermented beverage made from kombucha mushroom, brewed tea, and sugar. In recent years kombucha tea has attracted interest due to its pharmacological properties like antioxidant effects. The aim of the present research was to test the therapeutic effect of kombucha tea in EAE. We induced EAE model in 18 female C57BL/6 mice by inoculation of myelin oligodendrocyte glycoprotein-35-55 (MOG_35-55) in complete Freund’s adjuvant emulsion. Then, in order to ameliorate EAE symptoms, we used kombucha tea. During the course of study clinical evaluation was assessed, and on the day 21 post-immunization, for evaluation of nitric oxide (NO), total antioxidants capacity and tumor necrosis factor-alpha (TNF-α), blood samples were taken from the heart of mice. The mice were sacrificed and brains and cerebellums of mice were removed for histological analysis. Our findings demonstrated that kombucha tea had beneficial effects on EAE by lower incidence, attenuation in the severity, and also a delay in the onset of disease. Histological analysis showed that inflammatory criteria including the number of infiltrated immune cells and plaques as well as demyelination in kombucha tea dosed mice were significantly lower than the control group. Also, in comparison with control mice, the serum levels of NO and TNF-α in kombucha tea-treated mice were significantly decreased. Kombucha tea with its potential therapeutic effects and immunomodulatory properties might be proposed, after additional necessary tests and trials, for treatment of MS.     

DTF降茶氟剂降茶氟初步研究

目的探讨DTF制剂降茶氟效果。方法在不同制茶方法程序中加入DTF制剂观察其降氟效果。结果在冲泡或煮沸(1.0g茶叶,200ml水量)沏茶中,0.2gDTF可使茶氟降低约60%,使茶水氟浓度由2.0～4.0mg/L降到1.0mg/L左右,茶液色、味、pH值无改变。结论DTF是1种有效的降茶氟剂。     

茶水提取物和茶多酚抑制诱变的类型及其机制

目的 :研究茶水提取物和茶多酚的去突变特征和机制 ,鉴别茶和茶多酚去突变的量效关系和抑菌关系 ,了解去突变剂与直接诱变剂(1_NP)、间接(Trp_P_1)诱变剂的抗突变作用方式和抑制效果。 方法 :用细胞外抑制诱变试验和改进型两次活化的Ames试验方法。 结果 :茶水提取物和与其相关的儿茶素等都存在非抑菌性的去突变效果 ,其中表没食子儿茶素没食子酸酯(EGCG)和茶黄素(TF)的效果最好。抗突变试验结果表明 ,茶水提取物对Trp_P_1( +S9)有显著的抗突变性 ,与Trp_P_1的混合液在非代谢活性条件时无诱变性 ;茶水提取物对1_NP( -S9)也有抑制活性 ,但比对Trp_P_1的抑制活性低(P<0.01) ,与1_NP混合物经代谢活化后有诱变性 ,且与非代谢活化的抗突变结果呈较高的相关性(r= -0.9694)。 结论 :茶多酚能抑制间接诱变剂的前体形成 ,也有对直接诱变剂构成阻断剂的作用 ,但是在阻断具有强氧化性的诱变剂时可能形成不稳定的结合物或不安全的结构物。     

In vitro anti‐adhesive activity of green tea extract against pathogen adhesion

Camellia sinensis polysaccharide has been reported to possess anti‐adhesive activity against pathogens. The present study was designed to investigate whether hot water extracts obtained from green tea leaves might inhibit pathogen adhesion to human or mouse cell lines. Green tea extract‐4 (CSI‐4) with the maximum yield of 4% (w/v) is composed of a major proportion of carbohydrates containing 40% uronic acids, but lack of catechins. It showed strong inhibitory activities against hemagglutination mediated by pathogens Helicobacter pylori, Propionibacterium acnes and Staphylococcus aureus with the minimum inhibitory concentrations of 0.01‐0.5 mg/mL. CSI‐4 further demonstrated an inhibitory effect on the adhesion of these pathogens to host cell lines with the IC₅₀ values (50% inhibition of adhesion) of 0.14–2.3 mg/mL. It exhibited the highest activity against P. acnes, but no inhibitory effects were observed against Lactobacillus acidophilus, Bifidobacterium bifidum, Escherichia coli, or Staphylococcus epidermidis. Our results suggest that CSI‐4 may exert a selective anti‐adhesive effect against certain pathogenic bacteria with no adverse effects against beneficial or commensal bacteria. Copyright © 2008 John Wiley & Sons, Ltd.     

茶色素对冠心病及高血压病病人血小板功能的影响

目的：探索茶色素对冠心病、高血压病病人血小板功能的影响。方法：冠心病病人３６例（男性２１例，女性１５例；年龄６４±ｓ４ａ）；高血压病病人３０例（男性１８例，女性１２例；年龄５８±８ａ）。采用茶色素２５０ｍｇ，ｐｏ，ｔｉｄ，３０ｄ为一个疗程。结果：治疗后ＴＸＢ２下降，６－ｋｅｔｏ－ＰＧＰ１α上升，ＴＸＢ２／６－ｋｅｔｏ－ＰＧＦ１α比值下降（Ｐ＜０．０１或Ｐ＜０．０５），ＧＭＰ－１４０下降（Ｐ＜０．０５），ＰａｇＴ，ＰａｄＴ下降（Ｐ＜０．０５）。结论：茶色素具有降低血小板表面活性作用，抑制血小板聚集和粘附，抗血栓形成，改善微循环，对冠心病和高血压病病人起到积极的防治作用。     

茶多酚对老化相关酶GSH－Px、SOD及代谢产物过氧化脂质的影响

本实验研究了连续服用茶多酚２０天，对１４月龄小鼠血及肝中ＳＯＤ、ＧＳＨ－Ｐｘ和ＬＰＯ的影响。结果表明茶多酚能提高血及肝中ＳＯＤ及ＧＳＨ－Ｐｘ活性，与正常对照组比较Ｐ＜０．０１，同时能降低血及肝中ＬＰＯ含量，与正常对照组比较Ｐ＜０．０１。提示茶多酚可通过降低机体自由基水平，起到抗衰老作用。     

南岳毛尖抑制小鼠移植性S_(180)生长作用的初步研究

本研究用南岳毛尖(一级绿茶)浸泡液灌胃处理皮下移植S_(180)的Babl/c小白鼠,观察其抑瘤效果。结果表明该绿茶可明显抑制肿瘤的生长,瘤重明显轻于对照组(P<0.05);空泡变性瘤细胞数、瘤块内坏死灶数明显多于对照组(P<0.001);瘤细胞核分裂数明显少于对照组(P<0.005),对瘤细胞有较好的杀灭作用。该研究证明南岳毛尖是一种天然防癌抗癌物质,对干预肿瘤的发生和发展有重要意义。     

茶渣中蛋白质酶法提取工艺

研究了蛋白酶法提取茶叶加工后茶渣中蛋白质的工艺。结果显示，碱性蛋白酶和复合蛋白酶提取效果较好；碱性蛋白酶法提取的最佳工艺为酶加量49／6、液固比35：1（mL／g）、提取时间4h，提取率可达34．29，6；复合蛋白酶法提取的最佳提取工艺为酶加量39，6、液固比35：1（mL／g）、提取时间4h，提取率可达18．69／6；双酶法提取中，采用先复合蛋白酶，后碱性蛋白酶，提取效果较好，并且碱性蛋白酶占总酶加量比例对提取率的影响较大，当碱性蛋白酶占25％时，提取率达到最大，为42．19，6；双酶法提取的最佳提取工艺为pH8．0，温度60℃，酶加量49，6，提取率可达47．8％。     

茶叶多糖的研究进展

茶叶多糖是一种复合杂多糖，具有许多生物活性。作者系统地介绍了茶叶多糖的提取、分离、纯化、纯度鉴定及相对分子质量测定的方法，以及其含量和组成、理化性质、化学结构及空间构象、生物活性等方面的研究进展，并对上述方法及研究进展进行了分析和评述。