Maximizing intelligence obtained from higher-order DNA mixtures from volume crime samples

ABSTRACT

This work collates data from the analysis of complex mixtures analysed in STRmix during routine no-suspect volume crime work. It interrogates the upload rate for these types of mixtures and which component of the profile has been able to be interpreted for upload. The number of profiles giving multiple uploads and the amount of replicate PCR analysis has been collated.     

Prognostic and clinicopathological utility of programmed death-ligand 1 in malignant pleural mesothelioma: A meta-analysis

ObjectiveProgrammed death ligand 1 (PD-L1) has been reported to be connected to prognosis in individuals with malignant pleural mesothelioma (MPM), although there is no consensus based on data from previous studies. Accordingly, this quantitative meta-analysis investigated prognostic and clinicopathological utility of PD-L1 in patients with MPM.MethodsA comprehensive search of the PubMed, Web of Science, Embase, and Cochrane Library databases for articles published up to October 4, 2019 was performed. Studies using immunohistochemical techniques to detect/quantify the expression of PD-L1 in MPM tissue were enrolled in the analysis. The combined hazard ratio (HR) and corresponding 95% confidence interval (CI) was applied to assess the association between PD-L1 expression and overall survival (OS).ResultsA total of 11 studies comprising 1606 patients was included in the present meta-analysis. For OS, pooled data revealed an HR of 1.50 (95% CI 1.32–1.70; p < 0.001), suggesting that patients with PD-L1 overexpression experience inferior OS. Subgroup analysis revealed that elevated PD-L1 remained a significant prognostic indicator for worse OS, irrespective of sample size, cut-off value, ethnicity, and Newcastle-Ottawa Scale score. Moreover, PD-L1 overexpression was associated with non-epithelioid histology (odds ratio 4.30 [95% CI 1.89–9.74]; p < 0.001).ConclusionsResults of this meta-analysis show that elevated expression of PD-L1 could be a factor predicting poorer survival in patients with MPM.     

Inactivated influenza vaccine formulated with single-stranded RNA-based adjuvant confers mucosal immunity and cross-protection against influenza virus infection

Influenza vaccination is considered the most valuable means to prevent and control seasonal influenza infections, which causes various clinical symptoms, ranging from mild cough and fever to even death. Among various influenza vaccine types, the inactivated subunit type is known to provide improved safety with reduced reactogenicity. However, there are some drawbacks associated with inactivated subunit type vaccines, with the main ones being its low immunogenicity and the induction of Th2-biased immune responses. In this study, we investigated the role of a single-stranded RNA (ssRNA) derived from the intergenic region in the internal ribosome entry site of the Cricket paralysis virus as an adjuvant rather than the universal vaccine for a seasonal inactivated subunit influenza vaccine. The ssRNA adjuvant stimulated not only well-balanced cellular (indicated by IgG2a, IFN-γ, IL-2, and TNF-α) and humoral (indicated by IgG1 and IL-4) immune responses but also a mucosal immune response (indicated by IgA), a key protector against respiratory virus infections. It also increases the HI titer, the surrogate marker of influenza vaccine efficacy. Furthermore, ssRNA adjuvant confers cross-protective immune responses against heterologous influenza virus infection while promoting enhanced viral clearance. Moreover, ssRNA adjuvant increases the number of memory CD4⁺ and CD8⁺ T cells, which can be expected to induce long-term immune responses. Therefore, this ssRNA-adjuvanted seasonal inactivated subunit influenza vaccine might be the best influenza vaccine generating robust humoral and cellular immune responses and conferring cross-protective and long-term immunity.     

葛根素注射剂不良反应流行病学特点研究

目的 探讨葛根素注射剂不良反应的流行病学特点.方法 通过检索1978～2008年医药学期刊,收集葛根素注射剂不良反应详细病案120例;在构建数据库的基础上,应用统计学方法对其相关信息进行分析.结果 葛根素注射剂不良反应类型多样,其中以溶血反应数量最多,共32例,构成比为21.33%;其余依次为皮肤损害31例(20.67%),循环系统损害24例(16%),呼吸系统损害19例(12.67%),消化系统损害17例(11.33%),药物热11例(7.33%)和过敏性休克9例(6.00%)等.结论 溶血反应是葛根素注射剂最常见的不良反应;超剂量、不合理配伍、过敏性体质等可能是葛根素注射剂不良反应发生的主要原因.     

数据库技术新进展及其在医疗系统中的应用

论述了关系型数据库的革命性贡献与发展中的矛盾,实现医疗系统中的数据管理功能需要解决的问题,以及对临床数据输入系统的需求。     

医院数字化膳食管理系统的开发研制

目的：开发智能型的患者订餐工具和后台管理软件。方法：通过该院自编的应用程序读取医院信息系统(HIS)上患者的基本信息和饮食医嘱，利用掌上电脑和数据库开发技术，完成患者订餐及订餐后的信息管理。结果：系统投入运行后，订餐速度明显提高，差错率明显降低，患者就餐满意度也有所提高。结论：该系统的使用将明显提高该院膳食保障服务水平。     

阳江高本底地区居民健康流行病学调查数据库与统计分析方法

目的 研究应用计算机数据库技术及先进统计分析方法,对阳江高本底地区10多万人20余年的健康随访研究资料和剂量学调查资料进行储存、管理和分析。方法 通过调研和比较分析,确定采用Foxbase系列数据库管理系统按专题建立数据库,借助于ID号实现记录连接；一般统计分析由SAS完成；交叉分组人年列表由Epicure中的Datab完成；癌症相对危险分析用AMFIT程序。结果 建立了40MB的各专题研究库,主要包括：10余万人的人口学数据库(1979～1995)、1.2万人的死因数据库(1979～1995)和526个村庄6783户、5273人的剂量数据库。提出了动态群组研究资料(1979～1986)与固定群组研究资料(1987～1995)的合并方法：首选方法是建立假想的基于记录连接的以1979年为起点的(固定)群组。另一方法是将不同阶段群组研究的人年表分层直接相加。计算了每个成员基于卧室内、起居室内、室外村平均剂量率与性别-年龄别居留因子的累积剂量。完成了以性别、atainedage、随访年代及剂量交叉分组的人年列表,对癌症死亡资料(1979～1990)进行了危险分析。结论 应用计算机数据库管理系统与统计分析技术使阳江高本底地区研     

结直肠肿瘤多学科协作诊治模式的数据体系构建与运作策略

目的阐述在结直肠肿瘤多学科协作（MDT）诊治模式下,如何构建数据体系及其运作策略。方法阐明构建数据体系的原因,结合实践描述结直肠肿瘤数据体系的基本构成及如何支持数据体系的运行,探讨结直肠肿瘤MDT数据体系的价值反馈,并提出对数据体系建立未来的展望。结果通过会诊、随访、院内临床的支持及合理地实现MDT模式中信息化流程的建设,数据体系运行能正常进行。结论数据体系作为整个结直肠肿瘤MDT项目的基础,能够通过医疗模式的改变表现出医疗价值和社会价值。     

Access编制数据库在白内障病例管理中的应用

目的 应用微机对白内障病例进行管理，实现病例管理自动化。方法 根据患者姓名、晶状体等各项临床信息，在Windows98及以上版本的操作平台上，应用Access2000建立患者信息病例库。结果 建立Access病例库管理白内障病例，病例存储量大，查询方便快捷，数据处理方便。结论 Access2000编写的数据库在临床病例的管理中值得广泛应用及推广。