九里香的抗生育作用

九里香有抗炎、抗生育和治疗跌打损伤等功效,福建省莆田县民间验方用于引产。本文对九里香的抗生育作用进行了初步研究。观察了九里香不同药用部分、不同给药途径对小鼠各妊娠阶段的抗生育作用,及其对小鼠在体和离体子宫及前列腺素E_2合用的抗早孕作     

超微粉体技术对穿心莲药材中穿心莲内酯及脱水穿心莲内酯溶出率的影响

目的:探讨超微粉碎对穿心莲药材中有效成分溶出率的影响.方法:用高效液相色谱法(high performance liquid chromatography,HPLC)测定穿心莲超微粉与药材120目细粉中穿心莲内酯及脱水穿心莲内酯的溶出率.结果:超微粉药材中穿心莲内酯及脱水穿心莲内酯的溶出率明显增加.结论:超微粉碎可显著提高穿心莲药材中有效成分的溶出率.     

穿心莲的研究及临床开发

概述穿心莲在生药学、化学成分、药理学、临床应用、制剂开发、不良反应等方面的研究进展。穿心莲主要成分为二萜类内酯和黄酮，具有抗菌消炎、抗病毒、抗肿瘤、防治心血管疾病、抗肝损伤、增强免疫、抗生育等多种药理作用，值得进一步研究与开发。     

新穿心莲内酯对小鼠巨噬细胞呼吸爆发及淋巴细胞增殖的影响

目的: 观察新穿心莲内酯对小鼠RAW264. 7巨噬细胞呼吸爆发和BALB/C小鼠淋巴细胞增殖的影响,探讨其抗炎免疫活性。方法:分别用化学发光法和AlamarBlue染色法考察新穿心莲内酯对佛波豆蔻酸乙酯刺激的小鼠巨噬细胞呼吸爆发及植物凝集素刺激的BALB/C小鼠脾淋巴细胞增殖的影响。结果:新穿心莲内酯在 75 ~150μmol·L-1浓度范围内对刺激的巨噬细胞呼吸爆发及淋巴细胞增殖有抑制作用,而在 1. 5~7. 5μmol·L-1浓度范围内可协同刺激剂引起的呼吸爆发。结论:新穿心莲内酯在高浓度时具有抗炎活性,而低浓度时具有免疫刺激活性,提示其可能具有双向调节功能。     

穿心莲中穿心莲内酯、脱水穿心莲内酯在茎和叶中的分布

采用薄层扫描法分别测定了12批穿心莲商品药材中茎和叶所含穿心莲内酯和脱水穿心莲内酯的含量,并进行了比较,结果其总量在茎中平均占0.29%,在叶中平均占2.26%.因此建议穿心莲中叶所占比例应不少于35%.     

Andrographolide mitigates cardiac apoptosis to provide cardio‐protection in high‐fat‐diet‐induced obese mice

Excessive intake of high fat diet (HFD) and associated obese conditions are critical contributors of cardiac diseases. In this study, an active metabolite andrographolide from Andrographis paniculata was found to ameliorate HFD‐induced cardiac apoptosis. C57/BL6 mouse were grouped as control (n = 9), obese (n = 8), low dose (25 mg/kg/d) andrographolide treatment (n = 9), and high dose (50 mg/kg/d) andrographolide treatment (n = 9). The control group was provided with standard laboratory chow and the other groups were fed with HFD. Andrographolide was administered through oral gavage for 1 week. Histopathological analysis showed increase in apoptotic nuclei and considerable cardiac‐damages in the obese group signifying cardiac remodeling effects. Further, Western blot results showed increase in pro‐apoptotic proteins and decrease in the proteins of IGF‐1R‐survival signaling. However, feeding of andrographolide significantly reduced the cardiac effects of HFD. The results strongly suggest that andrographolide supplementation can be used for prevention and treatment of cardiovascular disease in obese patients.     

Andrographolide relieved pathological pain generated by spared nerve injury model in mice

Context: Andrographolide (Andro), found in large quantities in Andrographis paniculata Nees (Acanthaceae), is anti-inflammatory, especially in the central nervous system (CNS) glia.

Objective: The objective of this study is to test Andro’s ability to reduce allodynia in a spared nerve injury model.

Material and methods: Male 30?g BalbC mice were divided into four groups: (1) Sham-operated control (Sham-group); (2) nerve injured and treated with saline (Saline-group); (3) nerve injured and treated with Andro (Andro-group); (4) nerve injured and treated with non-steroidal anti-inflammatory drugs (NSAIDS) (NSAIDS-group). Andro or NSAIDS (diclofenac salt) were injected intraperitoneally at 5?mg/kg body weight daily. Mechanical allodynia was assessed by von Frey tests at 3, 7, and 14?d. For immunohistochemical analysis, samples were collected at 7?d.

Results: The threshold for inducing allodynia increased and the response percentage reduced in the Andro-group when compared with the Saline-group, as well as when compared with NSAIDS groups throughout 3–14?d. The ratio of threshold for OP-Andro/OP-saline and for OP-Andro/OP-NSAIDS groups was 20.42 and 11.67 at 14?d, respectively. The ratio of response percentage for OP-Andro/OP-saline and for OP-Andro/OP-NSAIDS was 0.32 and 0.39 at 14?d, respectively. Interleukin-1 (IL-1) immunostaining in the spinal cord was reduced in the Andro-group. Astrocytic activities were not significantly reduced in the Andro-group compared with the Saline-group at 7?d post-operation (PO)

Conclusions: Andro reduced mechanical allodynia more than NSAIDS at the same concentration, and the observed behaviour was associated with a reduction in inflammatory cytokine produced in the spinal cord.     

Effect of Andrographis paniculata as an Adjuvant in Combined Chemo-Radio and Whole Body Hyperthermia Treatment—A Preliminary Study

Modulation of immune responses to alleviate disease has been of interest for a long time. Intraperitoneal administration of Andrographis paniculata extract along with whole body hyperthermia (WBH) was found to enhance the total WBC count in cyclophosphamide (CTX) and radiation treated animals when compared to untreated control animals. Maximum inhibition in the solid tumor development was observed when the CTX and radiation exposed animals were treated with extract in combination with whole-body hyperthermia. Similarly myeloperoxidase activity in tumor tissue from CTX and radiation-treated animals was also significantly inhibited when they were administered with Andrographis paniculata extract along with whole body hyperthermia. Moreover the production of cytokines such as IL-2 and GM-CSF, which was reduced after combined CTX and radiation treatment was significantly increased by the simultaneous treatment of extract and whole body hyperthermia. The elevated level of serum Tumor Necrosis Factor (TNF-α) level, after CTX and radiation treatment was also lowered significantly after the administration of extract and simultaneous exposure of whole-body hyperthermia with respect to untreated tumor-bearing animals.     

穿心莲超分子提取物的抗炎作用机制研究

目的:研究穿心莲超分子提取物(SEA)的抗炎作用机制。方法:采用蛋清致炎模型测定炎症渗出液中丙二醛(MDA)含量和血清中SOD,CAT活力;醋酸所致小鼠腹腔炎症测定渗出液中PGE2的含量。结果:穿心莲超分子提取物能够显著降低炎症渗出液中PGE2和MDA含量,显著提高小鼠血清SOD和红细胞CAT活力。结论::穿心莲超分子提取物抗炎作用可能是通过抑制环氧化酶(COX)、抗自由基、抗氧化作用实现的。     

穿心莲优良种质筛选研究

搜集穿心莲种质资源20份,进行种植试验和生物学鉴定、评价及药用成分含量测定,结果表明:各地种植的穿心莲种子纯度不一致、混杂严重,主要存在大叶型和小叶型两种生态类型;不同种质的穿心莲茎叶干重、药用成分含量均差异显著,大叶型的茎叶干重及药用成分含量均明显高于小叶型,说明大叶型穿心莲的产量、产值较小叶型穿心莲具有明显的优势;经过2年的试验,初步筛选出产量中上等、药用成分含量高的优良品系。