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以“七麦数据”网站收录的中医移动医疗App作为研究对象,采用网络调查法和文献分析法,根据“七麦数据”对移动医疗App的分类,结合中医移动医疗App的信息服务内容和特点,将筛选出的中医移动医疗App划分为医疗健康类、中医养生类、知识传播类、全面综合类,并根据“七麦数据”网站中对各类中医移动医疗App的打分及累计下载量筛选出最具代表性的12款中医移动医疗App,从全面性、人性化、安全性、实用性4个一级指标和40个二级指标对其信息服务现状进行评价,指出当前中医移动医疗App信息服务存在的问题并提出建议。 相似文献
3.
High Order Finite Difference Hermite WENO Fixed-Point Fast Sweeping Method for Static Hamilton-Jacobi Equations 下载免费PDF全文
In this paper, we combine the nonlinear HWENO reconstruction in [43] and
the fixed-point iteration with Gauss-Seidel fast sweeping strategy, to solve the static
Hamilton-Jacobi equations in a novel HWENO framework recently developed in [22].
The proposed HWENO frameworks enjoys several advantages. First, compared with
the traditional HWENO framework, the proposed methods do not need to introduce
additional auxiliary equations to update the derivatives of the unknown function $\phi$.
They are now computed from the current value of $\phi$ and the previous spatial derivatives of $\phi$. This approach saves the computational storage and CPU time, which greatly
improves the computational efficiency of the traditional HWENO scheme. In addition,
compared with the traditional WENO method, reconstruction stencil of the HWENO
methods becomes more compact, their boundary treatment is simpler, and the numerical errors are smaller on the same mesh. Second, the fixed-point fast sweeping method
is used to update the numerical approximation. It is an explicit method and does
not involve the inverse operation of nonlinear Hamiltonian, therefore any Hamilton-Jacobi equations with complex Hamiltonian can be solved easily. It also resolves some
known issues, including that the iterative number is very sensitive to the parameter $ε$ used in the nonlinear weights, as observed in previous studies. Finally, to further
reduce the computational cost, a hybrid strategy is also presented. Extensive numerical experiments are performed on two-dimensional problems, which demonstrate the
good performance of the proposed fixed-point fast sweeping HWENO methods. 相似文献
4.
5.
目的 克隆菊花脑芳樟醇合酶基因CnTPS1的全长编码序列,利用原核系统表达融合蛋白,为进一步研究该基因在菊花脑萜类合成中的功能提供理论依据。方法 以菊花脑基因组数据为基础,设计特异性引物,PCR扩增CnTPS1的全长编码序列,利用生物信息学分析软件分析序列特征。利用qRT-PCR技术分析CnTPS1基因在不同组织中的基因表达量。构建原核表达载体,体外诱导目的蛋白表达。结果 CnTPS1编码序列全长1749bp,编码582个氨基酸;基因表达模式分析表明该基因在茎和管状花中表达量较高;原核表达系统能诱导出67.58kDa大小的蛋白。结论 首次从菊花脑中克隆得到一个芳樟醇合酶基因CnTPS1,运用生物信息学方法对其编码蛋白的理化性质、结构特征等进行了分析预测,分析了该基因的组织表达模式,并在原核表达系统中成功诱导表达出目标蛋白,这些结果将为菊花脑萜类合酶基因的功能以及萜类物质生物合成途径的解析提供理论依据。 相似文献
6.
目的对延续护理在重症监护病房(ICU)出院回家患者中应用的研究进行范围审查,以识别其延续护理内容及结局,为未来该领域的研究提供指导。方法检索PubMed、The Cochrane Library、Medline、CINAHL Complete、EMbase、中国知网(CNKI)、维普资源总库(VIP)、万方数据库(Wanfang Database)和中国生物医学文献数据库(SinoMed),检索时限为1980年1月—2021年3月,由2名研究员独立筛选、提取资料。结果纳入9篇文献。延续护理干预要素包括电话随访、家访、在线访视、门诊随访、同伴支持、健康教育、医院门诊或居家康复运动支持,一定程度上改善患者躯体功能、心肺功能和心理状况,提高生活自理能力和生活质量。结论多要素结合的延续护理对ICU直接出院回家患者结局起到积极影响,后续有待关注家属和探究科学的延续护理个案管理模式。 相似文献
7.
目的 探讨茯苓多糖对载脂蛋白E基因敲除(ApoE-/-)动脉粥样硬化(Atherosclerosis,AS)小鼠肝脏脂质沉积及胆固醇逆向转运的影响。方法 采用随机数字表法将30只ApoE-/-小鼠随机分为模型组、茯苓多糖组、辛伐他汀组,每组10只,10只C57BL/6J小鼠作为正常组。正常组给予基础饲料喂饲,其余小鼠给予高脂饲料喂饲12周。茯苓多糖组以0.2 g/kg/d灌胃,辛伐他汀组以2.275 mg/kg/天灌胃,正常组和模型组给予等体积生理盐水灌胃,共干预4周。全自动生化分析仪检测小鼠血清三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平,苏木素-伊红(HE)染色观察小鼠肝脏病理形态学变化,油红O染色观察小鼠肝脏脂质沉积情况,ELISA检测各组小鼠血清载脂蛋白A1 (ApoA1)、对氧磷酶-1(PON1)含量,Real-time RT-qPCR法及Western Blot法检测肝脏胆固醇酯转运蛋白(CETP)、磷脂转运蛋白(PLTP)、卵磷脂胆固醇酰基转移酶(LCAT)mRNA及蛋白表达。结果 与正常组比较,模型组小鼠血清TG、TC、LDL-C含量均明显上升,HDL-C含量明显下降(P<0.01),肝细胞脂肪变性明显,肝脏脂质沉积明显,小鼠血清ApoA1、PON1含量均明显下降(P<0.01),小鼠肝脏CETP、PLTP mRNA及蛋白表达明显上升,LCAT mRNA及蛋白表达明显下降(P<0.01)。与模型组相比,茯苓多糖组血清TG、TC、LDL-C含量均显著下降(P<0.01),HDL-C含量呈上升趋势(P>0.05),肝细胞脂肪变性、脂质沉积程度均有所减轻,茯苓多糖组ApoA1含量明显上升(P<0.01),PON1含量呈上升趋势(P>0.05),茯苓多糖组CETP、PLTP mRNA及蛋白表达有所下降,LCAT mRNA及蛋白表达有所上升(P<0.05,P<0.01)。结论 茯苓多糖可能通过调控血脂水平与胆固醇逆向转运过程,改善ApoE-/- AS小鼠肝脏脂质沉积,进而发挥防治AS作用。 相似文献
8.
目的:通过磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)/哺乳动物雷帕霉素靶蛋白(mTOR)信号通路,探讨炙甘草汤干预心肌缺血再灌注损伤(MIRI)致心律失常(室速和室颤)的作用及其机制。方法:将72只SD大鼠随机分为假手术组,模型组,炙甘草汤低、中、高剂量组(11.43,22.86,45.72 g·kg~(-1)),稳心颗粒组(2.43 g·kg~(-1)),连续给药干预10 d。末次给药2 h,采用结扎冠状动脉左前降支法制备大鼠MIRI模型,记录心电图的变化。造模成功后采集血液及心脏组织,检测血清中肌酸肌酶(CK),乳酸脱氢酶(LDH)及丙氨酸氨基转移酶(AST)的含量;酶联免疫吸附测定(ELISA)检测心肌肌钙蛋白(CtnI)的含量;免疫组化法检测心肌PI3K,Akt,mTOR的表达;采用蛋白免疫印迹法(Western blot)检测心肌自噬相关蛋白微管相关蛋白1轻链3(LC-3),自噬标志物Beclin1和PI3K/Akt/mTOR信号通路相关蛋白表达及磷酸化p-PI3K,p-Akt,p-mTOR的水平。结果:模型组大鼠100%发生室速,91.67%发生室颤;与假手术组比较,模型组大鼠血清CK,LDH,AST以及CtnI的含量显著升高(P0.01),心肌组织中PI3K,Akt,mTOR表达显著升高(P0.01),LC3-Ⅱ/LC3-Ⅰ与Beclin1的相对表达量显著升高(P0.01),p-PI3K/PI3K,p-Akt/Akt,p-mTOR/mTOR显著降低(P0.01);与模型组比较,炙甘草汤高剂量组室速、室颤发生率显著降低(P0.01),较其他给药组持续时间短(P0.01);炙甘草汤高剂量组CK,LDH,AST水平及CtnI含量显著降低(P0.01);炙甘草汤给药组的PI3K,Akt,mTOR的表达随剂量的增加而显著降低(P0.01);LC-3,Beclin1的表达随炙甘草汤各剂量组的增加有不同程度地下降(P0.01),而PI3K/Akt/mTOR相关蛋白表达比值明显升高(P0.05,P0.01)。结论:炙甘草汤预处理能使异常升高的心肌酶CK,LDH,AST,CtnI降低,抑制细胞的过度自噬,上调PI3K,Akt和mTOR的表达,说明炙甘草汤抗MIRI致心律失常的作用可能与PI3K/Akt/mTOR信号通路有关。 相似文献
9.
Haiyan Cao Yulong Zhai Xiaoying Ji Yaya Wang Jing Zhao Jinliang Xing Jiaze An Tingting Ren 《Cancer science》2020,111(8):2789-2802
Telomeric repeat‐containing RNA (TERRA) is closely involved in the regulation of telomere length, which plays critical roles in tumorigenesis. However, the biological significance of TERRA in hepatocellular carcinoma (HCC) remains largely unknown. In this study, we found that HCC cells show a frequent downregulation of TERRA and its positive regulator TTAGGG repeat binding factor‐1 (TRF1), whereas the negative regulator TTAGGG repeat binding factor‐1 (TRF2) was upregulated. We found that TERRA, TRF1, and TRF2 contributed to poor prognosis of HCC patients. Importantly, we found that the downregulation of TERRA significantly promoted HCC cell growth and metastasis in vitro and in vivo, whereas the upregulation of TERRA showed an opposite effect. Mechanistically, downregulation of TERRA significantly increased telomerase activity and promoted telomere elongation. Moreover, the inhibitory effects of TERRA overexpression on the growth and metastasis of HCC cells were reversed by treatment with TA‐65 that activates telomerase activity. In contrast, the protumor effect of TERRA downregulation was reversed by treatment with TMPyP4 that inhibits telomerase activity. Our findings reveal that TERRA plays a critical role in HCC cell growth and metastasis, indicating that TERRA is a potential therapeutic target for HCC. 相似文献
10.
Heming Lu Yuying Wu Xu Liu Huixian Huang Hailan Jiang Chaohua Zhu Yuping Man Zhaohong Chen Xianfeng Long Qiang Pang Luxing Peng Xianglong Li Junzhao Gu Shan Deng Ligang Xing 《Oncology research》2020,28(9):929-944
This phase II randomized clinical trial aimed to assess the efficacy and toxicity of Endostar, an antiangiogenesis
inhibitor, combined with concurrent chemoradiotherapy (CCRT) for locally advanced cervical cancer (LACC).
Patients with LACC were randomly assigned to either CCRT plus Endostar (CCRT+E arm) or CCRT alone
(CCRT arm). All patients received pelvic intensity-modulated radiation therapy (IMRT) and brachytherapy.
Weekly cisplatin was administered concurrently with IMRT. Patients in the CCRT+E arm also received concurrent Endostar every 3 weeks for two cycles. The primary endpoint was progression-free survival (PFS)
and acute toxicities. The exploratory endpoint was the impact of vascular endothelial growth factor receptor-2
(VEGFR2) expression on long-term survival. A total of 116 patients were enrolled. Patients in the CCRT+E
arm and in the CCRT arm had similar acute and late toxicity profile. The 1- and 2-year PFS were 91.4% versus
82.1% and 80.8% versus 63.5% (p=0.091), respectively. The 1- and 2-year distance metastasis-free survival
(DMFS) were 92.7% versus 81.1% and 86.0% versus 65.1% (p=0.031), respectively. Patients with positive
VEGFR2 expression had significant longer PFS and overall survival (OS) compared with those with negative
VEGFR2 expression. Patients in the CCRT+E arm had significantly longer PFS, OS, and DMFS than those
in the CCRT arm when VEGFR2 expression was positive. In conclusion, CCRT plus Endostar significantly
improved DMFS but not PFS over CCRT alone. The addition of Endostar could significantly improve survival
for patients with positive VEGFR2 expression. 相似文献